Recombinant Proteins

Proteins

Recombinant Proteins

Human

Mouse

Humon

TNF ALPHA

FGFR1

EGFR

ICAM2

IL8

M-CSF

ASPARTATE BETA HYDROXYLASE

B3GALT5

CD47

CEACAM1

ERBB 3

HER2

PD-L1

CD36

CD3E

PTPRN

CD10

CD147

CUTA

GM CSF RECEPTOR ALPHA

ICAM1

IL22

LAMP2

ROR1

SIRP ALPHA

SYNTENIN

BDNF

CD44

SONIC HEDGEHOG

TMEM169

TRKB

ZFYVE27

CD33

CD83

CD99L2

CTLA4

CYSTATIN C

FGFR3

MET

PD L2

PD1

SEMA4B

TRKC

ACID PHOSPHATASE

AIF

ANGIOTENSIN CONVERTING ENZYME 2

B7H3

CCK4

CD105

CD200

CD200R

CD98

CLM 9

FAM3C

FCGRT

FXYD3

GAA

GOLPH2

HAPTOGLOBIN

ICOS LIGAND

JUNCTIONAL ADHESION MOLECULE 1

LTA

LY108

LYMPHOCYTE ACTIVATION GENE 3

NEUROLIGIN 4

NRG1

SIGIRR

TIE1

VCAM1

BAP31

BTN1A1

C20ORF30

CD2

CD22

CD272

CD32B

CD79B

CD84

DPP6

DR5

EPCAM

ESAM

FXYD2

FXYD6

GPR56

HEME OXYGENASE 2

HFE

IL20 RECEPTOR ALPHA

IL5RA

IL6R

INPP4A

KALLIKREIN 8

LRRC8A

MAL

MCK10

MIDKINE

MYELIN OLIGODENDROCYTE GLYCOPROTEIN

NEURONAL MEMBRANE GLYCOPROTEIN M6 A

PDGF RECEPTOR BETA

PIGR

HEK293T

E. coli

HEK293

Sf9

Insect

CHO

Baculovirus

Human

C-Myc/DDK

C-His

N-His

C-Fc

Tag Free

C-DDK/His

C-6His

N-GST and C-His

C-DDK

N-GST and C-HIS

C-mFc

C-Fc&His

C-6His-Avi

N-His-ABP (Albumin-Binding Protein)

N-Trx&His

C-HIS

C-6×His

C-Fc-Avi

C-Human Fc

C-hFc

His-tag

C-Mouse Fc

Myc-DDK

C-8His

N-6His

N-His-Trx

N-GST

C-Mouse Fc and 6×His

C-Avi-6His

N-Fc

C- 6×His

C-10His

C-Fc&AVI

C-Human Fc and 6×His

N-Trx

C-Fc/His

C-hFc&His

His-Tag

N-GST, C-His

N-His/DDK

C-Flag

C-HUMAN

C-MYC/DDK

N-6His-Avi

N-Mouse Fc and C-6×His

Tag-free

C-6xHis/AVI

C-AVI&His

C-FC

C-FC-6HIS&C-FC-Flag

C-FLAG-Fc

C-GST

C-His/DDK

C-hIgG2 Fc

C-hIgG4 Fc

C-mFC

N-6His-Flag

N-8His

N-8His&N-FLAG

N-Avi&6His

N-DDK/His

N-FC

N-Flag&His

N-GFP

N-His PDCD1(Pro21-Val170)

N-His-GST

N-TRX

N-TRXA

N-Trx-His

N-mFc

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

GPCR

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

ELISA

Stem cell relevant signaling - Wnt Signaling pathway

SDS-PAGE

Ion Channels: Potassium

Bioactivity

Ion Channels: Glutamate Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

Ion Channels: Cys-loop Receptors

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

e.g. Dulbecco's PBS).

Flow Cyt

ELISA.
Western Blot.

Ion Channels: Transient receptor potential

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Functional Assays.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

Cytokine-cytokine receptor interaction

Metabolic pathways

Cell adhesion molecules (CAMs)

Hematopoietic cell lineage

Pathways in cancer

MAPK signaling pathway

Endocytosis

Natural killer cell mediated cytotoxicity

Jak-STAT signaling pathway

Regulation of actin cytoskeleton

Focal adhesion

Axon guidance

Adherens junction

Calcium signaling pathway

Toll-like receptor signaling pathway

Hypertrophic cardiomyopathy (HCM)

Lysosome

Dilated cardiomyopathy

Leukocyte transendothelial migration

Type I diabetes mellitus

Systemic lupus erythematosus

ECM-receptor interaction

TGF-beta signaling pathway

Neuroactive ligand-receptor interaction

Apoptosis

Melanoma

T cell receptor signaling pathway

Alzheimer's disease

Prostate cancer

Viral myocarditis

Allograft rejection

Chemokine signaling pathway

Colorectal cancer

Adipocytokine signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Antigen processing and presentation

ErbB signaling pathway

Graft-versus-host disease

Autoimmune thyroid disease

Primary immunodeficiency

Bladder cancer

Drug metabolism - cytochrome P450

Retinol metabolism

Pancreatic cancer

PPAR signaling pathway

Asthma

SNARE interactions in vesicular transport

Androgen and estrogen metabolism

B cell receptor signaling pathway

Neurotrophin signaling pathway

RIG-I-like receptor signaling pathway

Starch and sucrose metabolism

Metabolism of xenobiotics by cytochrome P450

Amyotrophic lateral sclerosis (ALS)

Melanogenesis

NOD-like receptor signaling pathway

Drug metabolism - other enzymes

Glycosphingolipid biosynthesis - lacto and neolacto series

Porphyrin and chlorophyll metabolism

Purine metabolism

Tight junction

Huntington's disease

Endometrial cancer

Glioma

N-Glycan biosynthesis

Gap junction

Non-small cell lung cancer

Ascorbate and aldarate metabolism

Fatty acid metabolism

Fc epsilon RI signaling pathway

Pyrimidine metabolism

Renin-angiotensin system

Parkinson's disease

Fc gamma R-mediated phagocytosis

Pentose and glucuronate interconversions

Basal cell carcinoma

Complement and coagulation cascades

Oxidative phosphorylation

Hedgehog signaling pathway

Sphingolipid metabolism

Cardiac muscle contraction

GnRH signaling pathway

Keratan sulfate biosynthesis

Pathogenic Escherichia coli infection

Arachidonic acid metabolism

Chronic myeloid leukemia

Renal cell carcinoma

Riboflavin metabolism

Type II diabetes mellitus

Tyrosine metabolism

p53 signaling pathway

Glycerophospholipid metabolism

Nicotinate and nicotinamide metabolism

O-Glycan biosynthesis

Linoleic acid metabolism

Heparan sulfate biosynthesis

Small cell lung cancer

ABC transporters

Glycosphingolipid biosynthesis - globo series

Cytosolic DNA-sensing pathway

Nitrogen metabolism

Wnt signaling pathway

Vibrio cholerae infection

Notch signaling pathway

Chondroitin sulfate biosynthesis

VEGF signaling pathway

Acute myeloid leukemia

Dorso-ventral axis formation

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Long-term depression

Prion diseases

Amino sugar and nucleotide sugar metabolism

Glycosaminoglycan degradation

Olfactory transduction

Tryptophan metabolism

C21-Steroid hormone metabolism

Thyroid cancer

Vascular smooth muscle contraction

Glutathione metabolism

Steroid biosynthesis

Caffeine metabolism

Galactose metabolism

Glycerolipid metabolism

Inositol phosphate metabolism

Insulin signaling pathway

Phosphatidylinositol signaling system

Ether lipid metabolism

Progesterone-mediated oocyte maturation

Ubiquitin mediated proteolysis

alpha-Linolenic acid metabolism

beta-Alanine metabolism

Glycosphingolipid biosynthesis - ganglio series

Long-term potentiation

Other glycan degradation

Cell cycle

Lysine degradation

Primary bile acid biosynthesis

Propanoate metabolism

Taste transduction

Valine

leucine and isoleucine degradation

Glycine

Oocyte meiosis

serine and threonine metabolism

Pantothenate and CoA biosynthesis

Arginine and proline metabolism

Biosynthesis of unsaturated fatty acids

Butanoate metabolism

Glycolysis / Gluconeogenesis

Histidine metabolism

Limonene and pinene degradation

Pyruvate metabolism

Phenylalanine metabolism

Sulfur metabolism

Protein export

Base excision repair

Terpenoid backbone biosynthesis

Human

Human 293

CHO Stable

E. coli

HEK293

Mammalian

P.Pichia

1

2

Yes

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