Proteins

Proteins

Recombinant Proteins

CytoSections

Mass Spec Standards

Over-expression Lysates

Mutant Proteins

Human

IRF5

HMGA1

PQBP1

MORTALITY FACTOR 4 LIKE 2

MAX

HNF 4 ALPHA

TNF ALPHA

HNRNPD

IKK GAMMA

PPAR GAMMA

ESTROGEN RELATED RECEPTOR GAMMA

P53

HMG1

BETA CATENIN

MEIS2

NRG1

PAX8

PML PROTEIN

RNF14

SUPT5H

TGIF

APE1

CYCLOPHILIN E

HOPX

NONO

PROTEIN KINASE A REGULATORY SUBUNIT I ALPHA

SIGIRR

SOD2

BMAL1

CITED1

CONSTITUTIVE ANDROSTANE RECEPTOR

DAXX

ESTROGEN RECEPTOR 1

GROWTH ARREST SPECIFIC PROTEIN 7

KAT5

NACA

NUCLEOPHOSMIN

TBL1Y

VGL4

EDF1

HIP2

NFAT1

STAT1

STAT3

ZNF580

CAMKK2

NFYC

TCF4

EED

HMGB2

NCOA4

NFYA

RBPJK

DYNAMIN 2

ERG

GABPB2

MLX

NUCLEAR FACTOR 1

PCDH1

RUNX1T1

SATB1

SCML1

SMAD2

TCF3 / E2A

TGF BETA 1

ZNF205

ZNF447

AMINO TERMINAL ENHANCER OF SPLIT

ATAXIN 3

BACH1

CERAMIDE SYNTHASE 2

CNBP

CSEN

CTNNBIP1

ELOB

FE65

FOXO3

FOXP4

GMEB1

GTF2IRD1

GTF3C2

HAIRLESS

HIF1 BETA

IKB BETA

ING4

IRF3

KEAP1

MCM4

MSK1

NFKB P100 / P52

NIPP1

NME2

NR2E3

P38

P63

P73

PEROXIREDOXIN 3

PIRIN

POLR1D

PRRX1

IHC

WB

HEK293T

HEK293

E. coli

Sf9

CHO

Insect

C-Myc/DDK

C-MYC/DDK

N-His

N-GST and C-His

C-His

Tag Free

C-DDK

N-GST and C-HIS

N-GST

His-tag

Myc-DDK

N-His&SUMO

N-His-ABP (Albumin-Binding Protein)

N-MARI

N-6His

C-DDK/His

N-GST, C-His

N-His-GST

C-6×His

C-Fc

C-Fc/His

C-Mouse Fc and 6×His

C-mFc

N-6His-FLAG

N-Avi

N-His&Flag

N-His/DDK

Tag-free

Yes

MVPro

SDS PAGE

WB

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Ion Channels: Other

Adult stem cells

GPCR

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Potassium

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Cys-loop Receptors

ELISA

SDS-PAGE

Ion Channels: Glutamate Receptors

Ion Channels: Transient receptor potential

Ion Channels: ATP Receptors

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Ion Channels: Sodium

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

Ion Channels: Cyclic nucleotide gated

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Ion Channels: Calcium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Pathways in cancer

MAPK signaling pathway

Wnt signaling pathway

Cell cycle

TGF-beta signaling pathway

Toll-like receptor signaling pathway

Chronic myeloid leukemia

Metabolic pathways

Acute myeloid leukemia

Pancreatic cancer

Huntington's disease

Prostate cancer

Small cell lung cancer

T cell receptor signaling pathway

Adipocytokine signaling pathway

Thyroid cancer

Neurotrophin signaling pathway

Chemokine signaling pathway

Purine metabolism

Pyrimidine metabolism

Apoptosis

Cytosolic DNA-sensing pathway

Ubiquitin mediated proteolysis

Colorectal cancer

RNA polymerase

Basal transcription factors

RIG-I-like receptor signaling pathway

B cell receptor signaling pathway

Jak-STAT signaling pathway

Maturity onset diabetes of the young

Amyotrophic lateral sclerosis (ALS)

NOD-like receptor signaling pathway

Endometrial cancer

Renal cell carcinoma

Adherens junction

Natural killer cell mediated cytotoxicity

Spliceosome

Basal cell carcinoma

ErbB signaling pathway

Antigen processing and presentation

Insulin signaling pathway

Non-small cell lung cancer

Systemic lupus erythematosus

Endocytosis

Bladder cancer

PPAR signaling pathway

Nucleotide excision repair

Tight junction

Allograft rejection

Graft-versus-host disease

Melanogenesis

Type I diabetes mellitus

RNA degradation

p53 signaling pathway

Base excision repair

Alzheimer's disease

Notch signaling pathway

Axon guidance

Cytokine-cytokine receptor interaction

Epithelial cell signaling in Helicobacter pylori infection

Fc epsilon RI signaling pathway

Melanoma

Oocyte meiosis

Type II diabetes mellitus

Dilated cardiomyopathy

Hypertrophic cardiomyopathy (HCM)

VEGF signaling pathway

Focal adhesion

Primary immunodeficiency

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Glioma

DNA replication

Fc gamma R-mediated phagocytosis

Pathogenic Escherichia coli infection

Asthma

Circadian rhythm - mammal

Hematopoietic cell lineage

Viral myocarditis

Leukocyte transendothelial migration

Autoimmune thyroid disease

Cell adhesion molecules (CAMs)

Dorso-ventral axis formation

Neuroactive ligand-receptor interaction

Regulation of actin cytoskeleton

Cysteine and methionine metabolism

GnRH signaling pathway

Long-term depression

Hedgehog signaling pathway

Ribosome

Valine

Homologous recombination

Lysine degradation

Glycolysis / Gluconeogenesis

Long-term potentiation

mTOR signaling pathway

Aminoacyl-tRNA biosynthesis

Calcium signaling pathway

Glycerolipid metabolism

Glycine

Glyoxylate and dicarboxylate metabolism

Nicotinate and nicotinamide metabolism

Non-homologous end-joining

Progesterone-mediated oocyte maturation

Proteasome

leucine and isoleucine biosynthesis

leucine and isoleucine degradation

serine and threonine metabolism

Vascular smooth muscle contraction

Biosynthesis of unsaturated fatty acids

Ether lipid metabolism

Prion diseases

Human

E. coli

Human 293

1

2

Yes

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