pCas-Guide-EF1a-RFP vector (with Cas9 and RFP expression) for genomic target sequence cloning (10 µg)
Function | All-in-One CRISPR/Cas9 vectors |
pCas-Guide-EF1a-RFP vector (with Cas9 and RFP expression) for genomic target sequence cloning (10 µg)
Function | All-in-One CRISPR/Cas9 vectors |
Donor vector with AAVS1 homologous arms, C-terminal mRFP tag, Puro resistance, ready for transgene cloning
Function | AAVS1 donor vector |
AAVS1 donor vector with AAVS1 homologous arms, firefly luciferase under EF1a promoter, and Blasticidin selection (10 ug)
Function | AAVS1 donor vector |
pCas-Guide-CRISPRa-Scramble, scramble gRNA control in GE100055 vector (no selection marker/reporter)
Function | CRISPRa scramble control vector |
pLenti-Cas9-mGFP-P2A-Puro control plasmid containing the expression cassette for Cas9-mGFP fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
pCas-Guide-Puro-CRISPRi-Scramble, scramble gRNA control in GE100083 vector (with Puro selection)
pCas-Guide-Puro-CRISPRa-Scramble, scramble gRNA control in GE100081 vector (with Puro selection)
pCas-Guide-Nickase vector for gRNA cloning (with Cas9 H840A expression) (10 µg)
Function | Cas9 Nickase |
Catalytically inactive Cas9 (D10A/H840A) vector for gRNA cloning (10 µg)
Function | Cas9 Nickase |
pLenti-Cas9-Myc-DDK-P2A-Puro control plasmid containing the expression cassette for Cas9-Myc-DDK fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
Validated sgRNA targeting RFP dead mutant in GE100041C cells