Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant Proteins

Mutant ORF Clones

Native Proteins

Human

PGP9.5

FACTOR I

SENP2

PSMA

MONOACYLGLYCEROL LIPASE

AMINOACYLASE 1

CATHEPSIN L

CD10

LEUKOTRIENE A4 HYDROLASE

PROSTATE SPECIFIC ANTIGEN

SPG7

HAPTOGLOBIN

DPP3

MMP3

EPOXIDE HYDROLASE

FLIP

MMP13

PROTEASOME SUBUNIT ALPHA TYPE 6

USP44

CASPASE 3

DPP9

MALT1

CARBOXYPEPTIDASE M

HGF

PHOSPHORIBOSYL PYROPHOSPHATE AMIDOTRANSFERASE

PSMA7

OTUB2

NAPSIN A

C1S

TMPRSS4

CATHEPSIN B

USP7

CASPASE-7

PSMB9

CASPASE 1

ARTS1

CARBOXYPEPTIDASE A

OTUB1

SENP8

AMINOPEPTIDASE A

USP5

MMP2

PACE4

TMPRSS5

CASPASE 9

NLN

PSMA4

SIL1

CLPP

CARBOXYPEPTIDASE A2

CASPASE 8

ADAM15

PROTEASOME SUBUNIT BETA TYPE 4

C2

ECE1

KALLIKREIN 11

MIPEP

DPP10

PSMA2

UPA

PR3

PROTEASOME 20S BETA 7

AGTPBP1

CD13

KALLIKREIN 6

MASP2

DPP6

MMP7

PROTEIN Z

BACE1

L KYNURENINE HYDROLASE

USP16

USP40

CALPAIN 3

CALPAIN 5

CALPAIN 9

CD71

DPP8

PEPD

PEPSINOGEN II

XPNPEP3

CASPASE 10

ANGIOTENSIN CONVERTING ENZYME 1

ATG4B

PREPL

CASPASE 5

CNDP1

FACTOR X

PARK7

SEC11A

ADAMTS1

ADAMTS4

HABP2

LACTOFERRIN

MEST

MMP1

XPNPEP1

ATP DEPENDENT METALLOPROTEASE YME1L1

CTSA

SENP7

Human

Mouse

Rat

Monkey

Dog

Bovine

Pig

Bovine)

Cow)

Human (Expected from sequence similarity: Mouse

Human (Predicted: Mouse

Canine

Rabbit

Hamster

Porcine

Mouse (Predicted: Rat

Primate

Rat)

Zebrafish

Gorilla

Dog)

Gerbil

Gibbon

Human (Expected from sequence similarity: Dog

Human (Predicted: Rat

Rabbit)

Xenopus

Bat

Cow

Feline

Horse

Human (Predicted: Monkey

Rhesus Monkey)

X. tropicalis)

Chicken

Chimpanzee

Horse)

Human (Expected from sequence similarity: Mouse)

Human (Expected from sequence similarity: Rat

Human (Predicted: Chimpanzee)

Human (Predicted: Dog

Human (Predicted: Dog)

Human (Predicted: Monkey)

Monkey)

Mouse (Predicted: Monkey)

Pig)

Rat (Predicted: Mouse

Chicken)

Equine

Fish

Guinea Pig

Hamster)

Horse (Predicted: Pig)

Human (Expected from sequence similarity: Dog)

Human (Predicted: Bovine

Human (Predicted: Bovine)

Human (Predicted: Chicken

Human (Predicted: Hamster)

Human (Predicted: Mouse)

Human (Predicted: Pig

Human (Predicted: Rabbit

Human (Predicted: Rabbit)

Mouse (Expected from sequence similarity: Human

Mouse (Predicted: Chicken

Mouse (Predicted: Chimpanzee

Mouse (Predicted: Rat)

Mouse (Predicted: Xenopus

Mouse)

Orang-Utan

Orang-Utan (Predicted: Bat

Orang-Utan (Predicted: Hamster

Orang-Utan (Predicted: Horse)

Pig (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Cow

Rat (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Human)

Rat (Expected from sequence similarity: Pig

Rat (Predicted: Rabbit)

Rhesus Monkey

Sheep

Sheep)

Xenopus)

Yeast

WB

IHC

FC

IF

ELISA

IP

LMNX

ICC/IF

10k-ChIP

ID

Simple Western

R

PEP-ELISA

CyTOF-ready

FN

Immunoblotting

SISCAPA

ChIP

Electron Microscopy

ICC

Neutralize

Mouse

Rabbit

Goat

Sheep

Chicken

Guinea Pig

HEK293T

HEK293

E. coli

Sf9

Insect

Plasmids

Myc-DDK

mGFP

Tag Free

C-Myc/DDK

TurboGFP

C-MYC/DDK

C-His

N-His

N-GST and C-His

N-GST and C-HIS

C-6xHis

N-GST

N-His&C-His

C-DDK

C-6His

C-Fc

C-HIS

C-HUMAN

N-6His

N-Fc

N-GST, C-His

N-His-PDCD1(Pro21-Val170)

N-His/DDK

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

PE

APC

TRITC

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgG2c

IgG3

IgG2a, kappa

IgM

IgY

Monoclonal

Polyclonal

No

Yes

Indirect

Sandwich

Direct

TrueMAB

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

WB

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Nuclear Hormone Receptor

Embryonic stem cells

Cancer stem cells

Induced pluripotent stem cells

Ion Channels: Potassium

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Complement and coagulation cascades

Proteasome

Metabolic pathways

Alzheimer's disease

Lysosome

Pathways in cancer

Apoptosis

Parkinson's disease

Renin-angiotensin system

Huntington's disease

Antigen processing and presentation

Amyotrophic lateral sclerosis (ALS)

Hematopoietic cell lineage

Viral myocarditis

p53 signaling pathway

Wnt signaling pathway

Arachidonic acid metabolism

Systemic lupus erythematosus

NOD-like receptor signaling pathway

Prostate cancer

Colorectal cancer

Glutathione metabolism

RIG-I-like receptor signaling pathway

Neuroactive ligand-receptor interaction

Bladder cancer

Arginine and proline metabolism

Natural killer cell mediated cytotoxicity

Alanine

Focal adhesion

aspartate and glutamate metabolism

Epithelial cell signaling in Helicobacter pylori infection

Glycerolipid metabolism

Regulation of autophagy

Metabolism of xenobiotics by cytochrome P450

Leukocyte transendothelial migration

Endocytosis

MAPK signaling pathway

B cell receptor signaling pathway

GnRH signaling pathway

T cell receptor signaling pathway

Cytokine-cytokine receptor interaction

Melanoma

Purine metabolism

Renal cell carcinoma

Cyanoamino acid metabolism

Selenoamino acid metabolism

Taurine and hypotaurine metabolism

Cytosolic DNA-sensing pathway

Endometrial cancer

Non-small cell lung cancer

Pancreatic cancer

Small cell lung cancer

VEGF signaling pathway

Toll-like receptor signaling pathway

Protein export

Notch signaling pathway

Type I diabetes mellitus

Cardiac muscle contraction

Oxidative phosphorylation

Tryptophan metabolism

Hypertrophic cardiomyopathy (HCM)

Histidine metabolism

beta-Alanine metabolism

PPAR signaling pathway

Amino sugar and nucleotide sugar metabolism

Regulation of actin cytoskeleton

Allograft rejection

Autoimmune thyroid disease

Graft-versus-host disease

Hedgehog signaling pathway

Spliceosome

Neurotrophin signaling pathway

Other glycan degradation

Basal transcription factors

Folate biosynthesis

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Cell cycle

Oocyte meiosis

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Human

Neutrophils

Baculovirus

Human 293

Plasma

Luminex

96-well plate, Luminex

96-well plate

Neomycin

Puromycin

None

TA600150

TA700155

TA600400

TA700399

TA600053

TA600153

TA600158

TA600163

TA600273

TA700159

TA700160

TA700273

TA700378

TA600052

TA600151, TA600152, TA600153, TA600154, TA600155

TA600156

TA600157

TA600159, TA600160

TA600161, TA600162

TA600273, TA600274, TA600275, TA600276

TA600274

TA600378, TA600380

TA600379

TA600399, TA600401, TA600402

TA600424

TA600425

TA600426

TA600427

TA600428

TA600435

TA600436

TA600437

TA600438

TA600439

TA600568

TA700051, TA700053

TA700052

TA700150, TA700151, TA700152, TA700153, TA700154

TA700155, TA700190, TA700191

TA700156

TA700157

TA700158, TA700163

TA700161, TA700162

TA700273, TA700274, TA700275

TA700273, TA700276

TA700379

TA700400, TA700401, TA700402

TA700424

TA700425

TA700426

TA700427

TA700428

TA700435

TA700436

TA700437

TA700438

TA700439

TA700568

Carrier-free Antibodies

1

2

3

Yes

In Stock