Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

Recombinant Proteins

Mutant ORF Clones

CytoSections

Mass Spec Standards

cDNA Clone Sets

Mutant CytoSections

Mutant Proteins

Over-expression Lysates

Human

BTK

ALK

HER2

MEK1

RET

EGFR

FGFR1

MEK3

B RAF

AKT1

FGFR2

LKB1

SAPK4

CD117

JNK1

ERBB 3

CAMK2B

ERK2

MET

ERK5

CDK2

CHK2

MARK3

PROTEIN KINASE D2

FLT4

PKC EPSILON

BUBR1

MTOR

JNK2

AURORA A

LIM KINASE 1

PDGF RECEPTOR ALPHA

P38

TRKB

NEK6

PRAK

S6K1

RIP3

PAK4

TLK2

TRKC

FAK

TYRO3

ABL2

CAMKK2

PLK1

CSNK1G2

MCK10

PDGF RECEPTOR BETA

AKT2

CSNK1G1

TRIB3

VRK2

HIPK1

MEK2

RAF1

CCK4

MADM

PRKACB

ROR2

VEGF RECEPTOR 2

CAMK2D

TIF1 ALPHA

SGK196

ERBB 4

PLK4

RSK3

PIM2

VEGF RECEPTOR 1

CSNK1G3

FGFR3

MEK4

CHK1

PROTEIN KINASE Y LINKED

SRC

TRKA

HCK

CAMK2G

CDK15

CK1 EPSILON

DYSTROPHIA MYOTONICA PROTEIN KINASE

IKBKE

JAK2

JNK3

PTK9

PYK2

SGK1

AKT3

IRAK4

CDK1

IGF1 RECEPTOR

MAK

ROR1

ACTIVIN RECEPTOR TYPE IIA

ERK1

INTEGRIN LINKED KINASE

STK39

TNIK

FYN

GRK4

Human

Mouse

Rat

Dog

Monkey

Bovine

Human (Predicted: Mouse

Cow)

Chicken

Human (Expected from sequence similarity: Mouse

Pig

Canine

Rat)

Bovine)

Primate

Dog)

Hamster

Rabbit

Xenopus)

Canis

Human (Predicted: Mouse)

Xenopus

Porcine

Rabbit)

Rat (Expected from sequence similarity: Dog

Bat

Mouse (Predicted: Rat

Zebrafish

Chimpanzee

Horse)

Human (Predicted: Bat

X. tropicalis)

Drosophila

Equine

Human (Expected from sequence similarity: Cow)

Human (Expected from sequence similarity: Dog

Human (Expected from sequence similarity: Dog)

Human (Predicted: Bovine

Human (Predicted: Chicken

Human (Predicted: Rat)

Mouse (Expected from sequence similarity: Rat

Pig)

Chicken)

Fish

Gorilla

Human (Expected from sequence similarity: Mouse)

Human (Predicted: Bovine)

Human (Predicted: Monkey

Human (Predicted: Pig

Invertebrate

Mouse (Predicted: Bovine)

Mouse (Predicted: Chicken

Mouse (Predicted: Pig)

Mouse)

Orang-Utan

Rat (Expected from sequence similarity: Dog)

Rhesus Monkey

Rhesus Monkey)

C. elegans (Does not react with: Human

Cow

Drosophila)

Gibbon

Gibbon (Predicted: Mouse)

Guinea Pig

Hamster)

Horse

Horse (Predicted: Xenopus)

Human (Does not react with: Mouse)

Human (Expected from sequence similarity: Pig

Human (Expected from sequence similarity: Rat)

Human (Predicted: Goat

Human (Predicted: Monkey)

Human (Predicted: Rabbit

Human (Predicted: Rat

Human (Predicted: Rhesus Monkey)

Human (Predicted: Xenopus)

Mammalian

Monkey)

Mouse (Expected from sequence similarity: Human

Mouse (Predicted: Pig

Mouse (Predicted: Rat)

Mouse (Predicted: Rhesus Monkey)

Rabbit (Does not react with: Primate)

Rat (Does not react with: Primate)

Rat (Expected from sequence similarity: Human

Rat (Expected from sequence similarity: Mouse

Sheep

Tobacco hornworm

X. tropicalis

WB

IHC

IF

FC

IP

ELISA

LMNX

ICC/IF

Dot

10k-ChIP

PEP-ELISA

Simple Western

Immunoblotting

ChIP

CyTOF-ready

FN

SISCAPA

E

Electron Microscopy

ICC

RNAi

Mouse

Rabbit

Goat

Rat

Sheep

Chicken

HEK293T

HEK293

E. coli

Sf9

Insect

Baculovirus

CHO

Plasmids

Myc-DDK

Tag Free

mGFP

C-Myc/DDK

TurboGFP

C-MYC/DDK

N-His

DDK-His

C-His

C-DDK/His

C-DDK

C-Fc

N-GST and C-His

N-GST and C-HIS

His-tag

C-Fc&His

N-His-ABP (Albumin-Binding Protein)

N-GST

C-6His-Avi

C-6His

C-6×His

C- 6×His

C-Fc-Avi

C-Myc-DDK

C-hFc

C-mFc

N-10His-Flag

N-FUSION

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-XL5-DDK-His

pCMV6-XL6

pCMV6-AC

PCMV6-Neo

pCMV6-AC-Myc-DDK

KO Validated Antibodies

Unconjugated

Biotin

HRP

PE

APC

FITC

ATTO 655

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgG3

IgG1, lambda

IgG2b, kappa

IgG2c

IgY

IgG2a, kappa

IgM

IgM, kappa

Monoclonal

Polyclonal

No

Yes

Sandwich

Indirect

Direct

TrueMAB

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

WB

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Nuclear Hormone Receptor

Embryonic stem cells

Cancer stem cells

Induced pluripotent stem cells

Ion Channels: Potassium

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

MAPK signaling pathway

Pathways in cancer

ErbB signaling pathway

Focal adhesion

Neurotrophin signaling pathway

Prostate cancer

Fc epsilon RI signaling pathway

GnRH signaling pathway

Regulation of actin cytoskeleton

Pancreatic cancer

Progesterone-mediated oocyte maturation

Glioma

Colorectal cancer

Endocytosis

Insulin signaling pathway

Chemokine signaling pathway

Toll-like receptor signaling pathway

Melanoma

T cell receptor signaling pathway

Non-small cell lung cancer

Endometrial cancer

Calcium signaling pathway

Gap junction

Bladder cancer

Acute myeloid leukemia

Adherens junction

B cell receptor signaling pathway

VEGF signaling pathway

Oocyte meiosis

Long-term potentiation

Fc gamma R-mediated phagocytosis

Chronic myeloid leukemia

Vascular smooth muscle contraction

Renal cell carcinoma

Cytokine-cytokine receptor interaction

Axon guidance

mTOR signaling pathway

Melanogenesis

Epithelial cell signaling in Helicobacter pylori infection

Natural killer cell mediated cytotoxicity

Wnt signaling pathway

Adipocytokine signaling pathway

Long-term depression

Thyroid cancer

Tight junction

Cell cycle

NOD-like receptor signaling pathway

Apoptosis

RIG-I-like receptor signaling pathway

Type II diabetes mellitus

TGF-beta signaling pathway

Dorso-ventral axis formation

Prion diseases

Small cell lung cancer

Leukocyte transendothelial migration

Primary immunodeficiency

Amyotrophic lateral sclerosis (ALS)

Hedgehog signaling pathway

Olfactory transduction

Jak-STAT signaling pathway

p53 signaling pathway

Alzheimer's disease

Cytosolic DNA-sensing pathway

Vibrio cholerae infection

Hematopoietic cell lineage

Pathogenic Escherichia coli infection

Viral myocarditis

Regulation of autophagy

Phosphatidylinositol signaling system

Dilated cardiomyopathy

Taste transduction

PPAR signaling pathway

Circadian rhythm - mammal

Purine metabolism

Hypertrophic cardiomyopathy (HCM)

Basal cell carcinoma

Ubiquitin mediated proteolysis

Parkinson's disease

Nucleotide excision repair

Basal transcription factors

Non-homologous end-joining

Human

E. coli

Human 293

96-well plate, Luminex

96-well plate

Luminex

Neomycin

None

Puromycin

Recombinant Antibodies

TA600111

TA700334

TA700335

TA600209

TA600228

TA700365

TA600055

TA600063

TA600072

TA600180

TA600212

TA700070

TA700180

TA700209

TA700215

TA700225

TA700364

TA700469

TA600006

TA600009

TA600010

TA600011

TA600012

TA600050

TA600064

TA600071

TA600071, TA600072, TA600074, TA600076

TA600181, TA600182

TA600208

TA600208, TA600210, TA600211

TA600213, TA600214

TA600215

TA600216

TA600217

TA600225, TA600226, TA600227, TA600228

TA600227, TA600229

TA600334, TA600335, TA600336, TA600337

TA600338, TA600339, TA600340, TA600341

TA600364, TA600368

TA600365, TA600366, TA600367

TA600369

TA600370

TA600469, TA600472

TA600470

TA600471

TA600473

TA600474

TA600509, TA600510

TA600510

TA600511

TA600511, TA600513

TA600512

TA600521

TA700006

TA700009

TA700010

TA700011

TA700012

TA700055, TA700077

TA700062, TA700064

TA700063

TA700070, TA700071, TA700073

TA700070, TA700072

TA700111, TA700137, TA700138, TA700139

TA700181, TA700182

TA700208, TA700209

TA700210, TA700211, TA700212

TA700213, TA700214

TA700216

TA700217

TA700225, TA700226

TA700225, TA700227, TA700228, TA700229

TA700226

TA700241

TA700366

TA700367

TA700470

TA700471

TA700472

TA700473

TA700509

TA700509, TA700510

TA700511

TA700511, TA700512

TA700513

TA700521, TA700531

Carrier-free Antibodies

1

2

3

Yes

In Stock