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Lenti ORF clone of Des (Myc-DDK-tagged ORF) - Rat desmin (Des), (10 ug)
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Products
Lenti ORF clone of Des (mGFP-tagged ORF) - Rat desmin (Des), (10 ug)
| Type | Rat Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti ORF clone of Des (mGFP-tagged) - Mouse desmin (Des)
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti ORF clone of Des (Myc-DDK-tagged) - Mouse desmin (Des)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Desmin Mouse Monoclonal Antibody
| Applications | IHC |
| Reactivities | Human |
Desmin human recombinant protein, 0.1 mg
| Expression Host | E. coli |
Desmin Rabbit polyclonal Antibody
| Applications | WB |
| Reactivities | Mouse |
| Modifications | Unmodified |
Des CRISPRa kit - CRISPR gene activation of mouse desmin
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
DES CRISPRa kit - CRISPR gene activation of human desmin
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Des (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Des (untagged) - Mouse desmin (Des), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
Anti-Desmin [14G1C12], Mouse IgG1, kappa
| Applications | ELISA, IHC, WB |
| Reactivities | Human |
Desmin (DES) mouse monoclonal antibody, clone D33, Purified
| Applications | IF, IHC, WB |
| Reactivities | Chicken, Hamster, Human, Rat |
Anti-Desmin [14G1C12], Rabbit IgG, kappa
| Applications | ELISA, IHC, WB |
| Reactivities | Human |
| Modifications | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
Desmin (DES) mouse monoclonal antibody, clone D33, Supernatant
| Applications | IF, IHC, WB |
| Reactivities | Human |
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- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
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Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization. (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) - e.g. Dulbecco's PBS). (2)
- pH 7.4). For immunization purposes (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
