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Des (Myc-DDK-tagged) - Mouse desmin (Des)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Products
Des - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Des (tGFP-tagged) - Mouse desmin (Des)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Des (Myc-DDK-tagged ORF) - Rat desmin (Des), (10 ug)
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Desmin (DES) rabbit polyclonal antibody, Serum
| Applications | IF, IHC, WB |
| Reactivities | Bovine, Canine, Chicken, Goat, Hamster, Human, Mouse, Rat, Zebrafish |
Desmin human recombinant protein, 0.25 mg
| Expression Host | E. coli |
Desmin mouse monoclonal antibody, clone RD301, Purified
| Applications | IF, IHC, WB |
| Reactivities | Canine, Chicken, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish |
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Species
Reactivities
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Expression Hosts
Lentivirus
Vectors
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Conjugates
Modifications
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Mutants
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Families
- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization. (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) - e.g. Dulbecco's PBS). (2)
- pH 7.4). For immunization purposes (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
