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CBLB (NM_170662) Human Tagged ORF Clone
CAT＃: RC206047
CBLB (Myc-DDK-tagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB)
ORF Plasmid: tGFP
"NM_170662" in other vectors (6)
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CNY 10,784.00
| Cited in 2 publications. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cbl-b; Nbla00127; RNF56 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206047 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACTCAATGAATGGCAGAAACCCTGGTGGTCGAGGAGGAAATCCCCGAAAAGGTCGAATTTTGG GTATTATTGATGCTATTCAGGATGCAGTTGGACCCCCTAAGCAAGCTGCCGCAGATCGCAGGACCGTGGA GAAGACTTGGAAGCTCATGGACAAAGTGGTAAGACTGTGCCAAAATCCCAAACTTCAGTTGAAAAATAGC CCACCATATATACTTGATATTTTGCCTGATACATATCAGCATTTACGACTTATATTGAGTAAATATGATG ACAACCAGAAACTTGCCCAACTCAGTGAGAATGAGTACTTTAAAATCTACATTGATAGCCTTATGAAAAA GTCAAAACGGGCAATAAGACTCTTTAAAGAAGGCAAGGAGAGAATGTATGAAGAACAGTCACAGGACAGA CGAAATCTCACAAAACTGTCCCTTATCTTCAGTCACATGCTGGCAGAAATCAAAGCAATCTTTCCCAATG GTCAATTCCAGGGAGATAACTTTCGTATCACAAAAGCAGATGCTGCTGAATTCTGGAGAAAGTTTTTTGG AGACAAAACTATCGTACCATGGAAAGTATTCAGACAGTGCCTTCATGAGGTCCACCAGATTAGCTCTGGC CTGGAAGCAATGGCTCTAAAATCAACAATTGATTTAACTTGCAATGATTACATTTCAGTTTTTGAATTTG ATATTTTTACCAGGCTGTTTCAGCCTTGGGGCTCTATTTTGCGGAATTGGAATTTCTTAGCTGTGACACA TCCAGGTTACATGGCATTTCTCACATATGATGAAGTTAAAGCACGACTACAGAAATATAGCACCAAACCC GGAAGCTATATTTTCCGGTTAAGTTGCACTCGATTGGGACAGTGGGCCATTGGCTATGTGACTGGGGATG GGAATATCTTACAGACCATACCTCATAACAAGCCCTTATTTCAAGCCCTGATTGATGGCAGCAGGGAAGG ATTTTATCTTTATCCTGATGGGAGGAGTTATAATCCTGATTTAACTGGATTATGTGAACCTACACCTCAT GACCATATAAAAGTTACACAGGAACAATATGAATTATATTGTGAAATGGGCTCCACTTTTCAGCTCTGTA AGATTTGTGCAGAGAATGACAAAGATGTCAAGATTGAGCCTTGTGGGCATTTGATGTGCACCTCTTGCCT TACGGCATGGCAGGAGTCGGATGGTCAGGGCTGCCCTTTCTGTCGTTGTGAAATAAAAGGAACTGAGCCC ATAATCGTGGATCCCTTTGATCCAAGAGATGAAGGCTCCAGGTGTTGCAGCATCATTGACCCCTTTGGCA TGCCGATGCTCGACTTGGACGACGATGATGATCGTGAGGAGTCCTTGATGATGAATCGGTTGGCAAACGT CCGAAAGTGCACTGACAGGCAGAACTCACCAGTCACATCACCAGGATCCTCTCCCCTTGCCCAGAGAAGA AAGCCACAGCCTGACCCACTCCAGATCCCACATCTAAGCCTGCCACCCGTGCCTCCTCGCCTGGATCTAA TTCAGAAAGGCATAGTTAGATCTCCCTGTGGCAGCCCAACGGGTTCACCAAAGTCTTCTCCTTGCATGGT GAGAAAACAAGATAAACCACTCCCAGCACCACCTCCTCCCTTAAGAGATCCTCCTCCACCGCCACCTGAA AGACCTCCACCAATCCCACCAGACAATAGACTGAGTAGACACATCCATCATGTGGAAAGCGTGCCTTCCA AAGACCCGCCAATGCCTCTTGAAGCATGGTGCCCTCGGGATGTGTTTGGGACTAATCAGCTTGTGGGATG TCGACTCCTAGGGGAGGGCTCTCCAAAACCTGGAATCACAGCGAGTTCAAATGTCAATGGAAGGCACAGT AGAGTGGGCTCTGACCCAGTGCTTATGCGGAAACACAGACGCCATGATTTGCCTTTAGAAGGAGCTAAGG TCTTTTCCAATGGTCACCTTGGAAGTGAAGAATATGATGTTCCTCCCCGGCTTTCTCCTCCTCCTCCAGT TACCACCCTCCTCCCTAGCATAAAGTGTACTGGTCCGTTAGCAAATTCTCTTTCAGAGAAAACAAGAGAC CCAGTAGAGGAAGATGATGATGAATACAAGATTCCTTCATCCCACCCTGTTTCCCTGAATTCACAACCAT CTCATTGTCATAATGTAAAACCTCCTGTTCGGTCTTGTGATAATGGTCACTGTATGCTGAATGGAACACA TGGTCCATCTTCAGAGAAGAAATCAAACATCCCTGACTTAAGCATATATTTAAAGGGAGATGTTTTTGAT TCAGCCTCTGATCCCGTGCCATTACCACCTGCCAGGCCTCCAACTCGGGACAATCCAAAGCATGGTTCTT CACTCAACAGGACGCCCTCTGATTATGATCTTCTCATCCCTCCATTAGGTGAAGATGCTTTTGATGCCCT CCCTCCATCTCTCCCACCTCCCCCACCTCCTGCAAGGCATAGTCTCATTGAACATTCAAAACCTCCTGGC TCCAGTAGCCGGCCATCCTCAGGACAGGATCTTTTTCTTCTTCCTTCAGATCCCTTTGTTGATCTAGCAA GTGGCCAAGTTCCTTTGCCTCCCGCTAGAAGGTTACCAGGTGAAAATGTCAAAACTAACAGAACATCACA GGACTATGATCAGCTTCCTTCATGTTCAGATGGTTCACAGGCACCAGCCAGACCCCCTAAACCACGACCG CGCAGGACTGCACCAGAAATTCACCACAGAAAACCCCATGGGCCTGAGGCGGCATTGGAAAATGTCGATG CAAAAATTGCAAAACTCATGGGAGAGGGTTATGCCTTTGAAGAGGTGAAGAGAGCCTTAGAGATAGCCCA GAATAATGTCGAAGTTGCCCGGAGCATCCTCCGAGAATTTGCCTTCCCTCCTCCAGTATCCCCACGTCTA AATCTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC206047 protein sequence
Red=Cloning site Green=Tags(s) MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNS PPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDR RNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSG LEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKP GSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPH DHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEP IIVDPFDPRDEGSRCCSIIDPFGMPMLDLDDDDDREESLMMNRLANVRKCTDRQNSPVTSPGSSPLAQRR KPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPPE RPPPIPPDNRLSRHIHHVESVPSKDPPMPLEAWCPRDVFGTNQLVGCRLLGEGSPKPGITASSNVNGRHS RVGSDPVLMRKHRRHDLPLEGAKVFSNGHLGSEEYDVPPRLSPPPPVTTLLPSIKCTGPLANSLSEKTRD PVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCMLNGTHGPSSEKKSNIPDLSIYLKGDVFD SASDPVPLPPARPPTRDNPKHGSSLNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPG SSSRPSSGQDLFLLPSDPFVDLASGQVPLPPARRLPGENVKTNRTSQDYDQLPSCSDGSQAPARPPKPRP RRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNVEVARSILREFAFPPPVSPRL NL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_170662 |
| ORF Size | 2946 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_170662.2 |
| RefSeq Size | 3976 bp |
| RefSeq ORF | 2949 bp |
| Locus ID | 868 |
| UniProt ID | Q13191 |
| Protein Families | Druggable Genome |
| Protein Pathways | Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis |
| MW | 109.4 kDa |
| Gene Summary | This gene encodes an E3 ubiquitin-protein ligase which promotes proteosome-mediated protein degradation by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate. The encoded protein is involved in the regulation of immune response by limiting T-cell receptor, B-cell receptor, and high affinity immunoglobulin epsilon receptor activation. Studies in mouse suggest that this gene is involved in antifungal host defense and that its inhibition leads to increased fungal killing. Manipulation of this gene may be beneficial in implementing immunotherapies for a variety of conditions, including cancer, autoimmune diseases, allergies, and infections. [provided by RefSeq, Sep 2017] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
LncRNA DUXAP9-206 directly binds with Cbl-b to augment EGFR signaling and promotes non-small cell lung cancer progression
,Zhu, T;An, S;Choy, MT;Zhou, J;Wu, S;Liu, S;Liu, B;Yao, Z;Zhu, X;Wu, J;He, Z;,
J. Cell. Mol. Med.
,PubMed ID 30515972
[CBLB]
|
|
CBL mutations in myeloproliferative neoplasms are also found in the gene’s proline-rich domain and in patients with the V617FJAK2
,Paula Aranaz, Cristina Hurtado, Ignacio Erquiaga, Itziar Miguéliz, Cristina Ormazábal, Ion Cristobal, Marina García-Delgado, Francisco Javier Novo, and José Luis Vizmanos,
Haematologica, Aug 2012; 97: 1234 - 1241.
[CBLB]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206047L1 | Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB), Myc-DDK-tagged |
CNY 13,184.00 |
|
| RC206047L2 | Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB), mGFP tagged |
CNY 5,890.00 |
|
| RC206047L3 | Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB), Myc-DDK-tagged |
CNY 13,184.00 |
|
| RC206047L4 | Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB), mGFP tagged |
CNY 13,184.00 |
|
| RG206047 | CBLB (tGFP-tagged) - Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB) |
CNY 12,384.00 |
|
| SC107022 | CBLB (untagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB) |
CNY 10,800.00 |
