ANKRD6 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6G8]

Specifications

Product Data
Clone Name	6G8
Applications	ELISA, WB
Recommend Dilution	ELISA: 0.1 mg/ml (protein ELISA). Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. This product contains a Positive Control for Immunoblot Applications (for details see "Protocols")
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH. Epitope: amino acids 100-123.
Specificity	This antibody specifically recognizes Diversin at 75 kDa.
Formulation	PBS containing 0.09% Sodium Azide, PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	ankyrin repeat domain 6
Database Link	Entrez Gene 22881 Human UniProt ID Q9Y2G4
Background	Diversin 1 is a 75 kDa protein that acts in both canonical Wnt and Wnt/JNK signaling. Diversin recruits casein kinase 1 epsilon (CK1ε) to the beta-catenin degradation complex of axin/conductin and GSK3beta, thus leading to N-terminal phosphorylation and subsequent degradation of beta-Catenin.
Synonyms	KIAA0957
Note	Protocol: Positive Control: SKOV-3 Untreated Formulation: Lyophilized Cell Lysate from Serum starved SKOV-3 Cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control cell lysate is recommended for immunoblot applications. 20µl of positive control cell lysate corresponds to ca 20.000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch.
Reference Data

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