gRNA克隆和Donor构建

gRNA克隆服务     Donor载体构建


CRISPR/Cas9 定制服务


Custom Services Reference Price
Target sequence cloning into CRISPR/Cas9 vectors $345
$175
Donor vector construction with a predesigned cassette ~$880
1.2kb of homologous arms (left + right)
Donor vector of your own design Gene synthesis cost, $0.4-0.5/bp

预制功能性Donor 元件:

  • GFP-LoxP-PGK-Puro-LoxP
  • RFP-LoxP-PGK-BSD-LoxP
  • Luc-LoxP-PGK-Puro-LoxP
  • mBFP-LoxP-PGK-Neo-LoxP

Cre表达载体用于Cre-loxp重组