pMirTarget 3' UTR Assay Vector

CAT#: PS100062

pMirTarget, the cloning vector for all 3' UTR clones for miRNA target validation, 10ug


 Product Datasheet for 'PS100062'

CNY 5,420.00


货期*
现货

规格
    • 10 ug

Cited in 51 publications.

Product images

Specifications

Product Data
sds_url /sds/ps100062.pdf
_pt 1
expression Mammalian
_c 3-utr-assay-vector
availabilities_filter In Stock
ecoli_selection Kanamycin
sub_category 3' UTR Assay Vector
fpfeature Green, dimer
multiple_cloning /assets/images/trueorf/ps100062-mcs.gif
description pMirTarget, the cloning vector for all 3' UTR clones for miRNA target validation, 10ug
pdf_url /datasheet/ps100062.pdf
product_name pMirTarget 3' UTR Assay Vector
features

pMirTarget is a reporter construct with firefly luciferase as a reporter. The multiple cloning sites are downstream of luciferase stop codon and allow insertion of any sequence of interest, creating a chimerical transcript. All of OriGene’s 3' UTR clones are constructed in this vector. In the presence of appropriate miRNA (external or internal), shRNA or siRNA, the RNAi machinery would interact with the 3’UTR sequence and causes reduction of the luciferase activity.

  • High sensitivity of Luciferase assay due to IRES-driven luciferase cassette
  • RFP as a reporter for transfection monitoring and normalization
  • Neomycin selection marker for stable cell establishment
  • Compatible with Precision shuttling system
  • Can serve as a control for target validation experiments.
  • 3' UTR Clones for MicroRNA target validation
  • LUC_F Primer can be used to sequence the target sequence after cloning.
cell_selection Neomycin
citations Yes
vector_seq_file assets/documents/trueorf/ps100062.doc
terms LUCIFERASE;PCMV
name pMirTarget
vector Destination Vector
category Vectors
sizes_filter 10 ug
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (51)

Documents