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抗体
OriGene 拥有成熟的抗体研发平台和抗体鉴定平台。目前可提供丰富的抗体产品，产品的数量多，类型丰富，基因覆盖度高，可满足科学研究者不同的实验需求。
OriGene拥有超过90,000种一抗以及常用的二抗和标签抗体，抗体种类多样，包含多种种属。通过先进的技术制备免疫原，获得抗体后经过多重验证。我们高品质的抗体品牌包括：TrueMAB®, UltraMAB®, 和 TrueRAB®
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优势一抗品牌
UltraMAB®
The Ultra Specific Antibodies Specificity tested by High-Density Protein Microarray Designed for anatomic pathology Focused on cancer research and diagnostics
TrueMAB®
Mouse Monoclonal Antibodies Full-length human proteins as antigens Recognize native epitopes of target proteins Extensive validations for WB, IHC, IF/ICC, IP, ELISA & Flow
TrueRAB™
Rabbit Monoclonal antibodies Higher sensitivity comparing to other species Smaller antigen for the accurate epitope mapping Extensive validations for WB, IHC, IF/ICC, IP, ELISA & Flow
抗体特异性验证方案
产品特异性是抗体特性非常重要的一个方面。OriGene 在抗体开发过程中，采用多种方式来进行产品特异性验证。
验证方式
- 根据预测分子量大小，通过Western blot进行分析
- 独立抗体特异性验证策略
- 10k /17K高密度蛋白芯片验证
- 使用CRISPR技术敲除细胞系（KO）验证
验证数据示例
Specificity Validation for OriGene antibody Cat. No. UM500036
Figure 1. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using human Her2 overexpression cell lysates and endogenous cell line lysates. UM500036 can recognize the specific Her2 protein at predicted size (~140KDa) in overexpression cell ysate (lane 1 ) and endogenous lysates prepared from Hela, A549 and MCF7 cell lines. The specificity is further proved by using an independent antibody (anti-DDK antibody in the middle) against the same recombinant overexpressed Her2 protein.
Figure 2. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using 10k protein chip technology. UM500036 shows no cross-reactivity with other protein on the tested protein microarray containing >10,000 overexpression lysates for different human proteins.
Figure 3. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using Her2 knockout
cell lysate. UM500036 recognizes the specific Her2 protein at correct size in wild type Hela cell lysate
(WT lane), and shows cross-reactivity with any other protein in the double knockout cell lysates (KO lane).
WT: parental wild type Hela cell lysate
KO: Her2 double knockout Hela cell lysate
近期文献应用
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1. Saikosaponin A protects against uremic toxin indole‑3 acetic acid‑induced damage to the myocardium Published in Molecular Medicine Reports, 2023 Sep, using TA130015 |
PubMed: 37417356 |
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2. Targeting ANXA7/LAMP5-mTOR axis attenuates spinal cord injury by inhibiting neuronal apoptosis via enhancing autophagy in mice Published in Cell Death Discovery, 2023 Aug, using R1107 |
PubMed: 37620352 |
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3. A pH‑responsive hyaluronic acid nano‑vehicle co‑encapsulating doxorubicin and all‑trans retinoic acid for the inhibition of hepatic stellate cell‑induced tumor growth and metastasis Published in Molecular Medicine Reports, 2023 Aug, using TA130015 |
PubMed: 37326031 |
| 抗体相关资料 |
