TFF1 Mouse Monoclonal Antibody [Clone ID: C-3D5]

Specifications

Product Data
Clone Name	C-3D5
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200). Immunohistochemistry on Paraffin Sections: 0.4-2 µg/ml (1/100-1/500). Microwave pretreatment for antigen retrieval is required. Suggested Positive Control: Human Lung Tissue, Swine Stomach.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide C57 to F84 from Human Trefoil Factor 1. Antigen/Epitope: The antigen is Trefoil Factor 1, the epitope is located in the C-terminal region.
Specificity	This Monoclonal antibody detects Human/Porcine Trefoil Factor 1 (TFF1). Antigen Distribution: The antigen has been described in stomach, with highest levels in the upper gastric mucosal cells. Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the. Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system. Detected in urine. Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (Swissprot).
Formulation	Stock solution contains 0.2 mg/ml IgG, PBS, pH 7.2 with 5 mg/ml BSA as stabilizer and 0.01% Kathon as a preservative State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the lyophilized antibody at 2- 8°C. Following reconstitution, store at -20°C. Avoid repeated freezing and thawing.
Gene Name	trefoil factor 1
Database Link	Entrez Gene 7031 Human UniProt ID P04155
Background	Trefoil Factor 1 has been described as a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. TFF1 may inhibit the growth of calcium oxalate crystals in urine.
Synonyms	TFF1, BCEI, Breast cancer estrogen-inducible protein, PNR-2
Reference Data
Protein Families	Druggable Genome, Secreted Protein

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