Pdgfb (BC064056) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	PDGF-B; Sis
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC064056 GCTTTCTGATCTGGGGGTCCGTTCCAGAAAATGTTGCAAAAAAGCTAAGCCAGCAGGCAGAGGAACACGC CTTTAGCGGGCGAGTGAAGACGAACCATCGGCTGCCGTGTTCCTTTTCCTCTCTGAGGTTGGAGTCCCCT GCGCGCCCCCACACGGCTAGACGCCTTGGCTGGTTCGCGACGCAGCCCCCAGACTGTGGATGCTCGCGTG GGCTCGGGATCCGCCCAGGTAGCGGCTTTGGACCCCGGTCCCTTGTCCAGGTCCTCCCCAGCCCCCCAGC GACGGAGCCGGGGCCGGGGGCGGCGGCGCCCGGGGGCCATGCGGGTGAGCCGCGGCGGCGGCTGCAGCGG CCTGATCGCCTGATCGCGGGGGACCCGAGCGGAGCCCACCCTCCTCCCCAGCCCCCAACCCTGGCCGCGG GGGCGGCGCGCTCCGTCTACGCGTCCGGGGCCCCGCGGGGCCGGGCCCGGAGTCGGCATGAATCGCTGCT GGGCGCTCTTCCTTCCTCTCTGCTGCTACCTGCGTCTGGTCAGCGCCGAGGGGGATCCCATTCCTGAGGA ACTGTATGAAATGCTGAGCGACCACTCCATCCGCTCCTTTGATGATCTTCAGCGCCTGCTGCACAGAGAC TCCGTAGATGAAGATGGGGCTGAGCTGGACTTGAACATGACCCGAGCACACTCCGGAGTCGAGTTGGAAA GCTCATCTCGAGGGAGGAGGAGCCTAGGGTCCCTGGCAGCAGCAGAGCCTGCTGTAATCGCCGAGTGCAA GACGCGCACAGAGGTGTTCCAGATCTCTCGGAACCTCATCGATCGCACCAACGCCAACTTCCTGGTGTGG CCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAATAACCGCAATGTGCAATGCCGGGCCTCGC AGGTGCAGATGCGGCCGGTCCAGGTGAGAAAGATTGAGATTGTGCGAAAGAAGCCCATCTTCAAGAAGGC CACAGTGACCTTGGAGGACCACCTCGCCTGCAAGTGTGAGACAGTAGTGACCCCTCGGCCTGTGACTAGA AGTCCTGGGACATCCAGGGAGCAGCGAGCCAAGACGCCTCAAGCTCGGGTGACCATTCGGACGGTGAGAA TCCGCCGGCCCCCCAAAGGCAAGCACCGAAAGTTTAAGCACACCCATGACAAGGCGGCCCTGAAGGAGAC CCTCGGAGCCTAGGGGCGTCGGCAAGAGTGTGGGCAGGGTTATTTAATATGGTATTTGCTGTATTGCCCC CATGGGGTCCTTGGAGTGATAATGTTGTTCCCCTCGTCCGTCTGTCTCGATGCCTGATTCGGACGGCCAA TGGTGCTTCCCTCACCCCGTGTGCCCGGCCACCTCCACCAGCAGCGATTCCTCAGCCCGCCCTGCAGCTC CAGATGCAAAGGACTCCACTCCAGGCTGCAGGCTTCTCTTGACTCCAAGAACCTGGAACAAGTGTGCAGG AGCCTTGCCGGGGGACGTGGACTGTGGACTGGCCCCAGAGCCTGGCACTTAGCCCGAGGACCTCTGCATG TCCTGCCTGGTTCCTGGACCACTGGCCAGCCAGCAGGGAACATGTTCAGGCAGGCTAGGGTCCCTTGCAG TCCTGTGGCAGGGAGCACAGACTGGAGGAACTCTGATGGCACCCAGATCTGCCACGCACTCATCTCTTCC TGTCCCCTCAGCCCACAGTGGCTTTTCATTTTATAAGACATGAACTCCTCTGGGCAGGTGGCCACACACC TTCTCTGATGGATTAGAGGTGCAGTGTGCGTGTGACCTGGGCCGTGTCCCTGCCTCTTTCCACTTCCCCT ACGTTCACTTCCGGTTCATTTCTCTACCTCCACTCTGTGTCTTCTTCCTCATGTGCCCTTCAGTCTGCTC CTCCACGGGGTGTTCGGATCCCGATTGAGGCCCTGAATGCCCGCCATTCCTCCCTAGGGCAGGAGACCTG TCTCTTATATTCCACTCCAGCCAAGCAGGGTGAGCAAGGTTGTAATGGGATGTATATTGCACATGATGCA CTGCGTGTGGAGACTGCTGTTCACACCCTAAATATGGCCAACTTGCCTATTATGACGTTCTGGCCAATGT AGGTCAAACCTCTGAGGAAAGGGAACCTTGTTTAATTCATACCGAGGCCCTCTGTGGAGTGGCTGTGTGT GCCCTGGCCCAGCCTGAAGCTCAGAATGAGCCCATGGTACTGGGGAAGCAACCCTGTTGTCTGTCCATTG CCTCCACTTCCTGGGTCTCAGCTGTCCCCTTCCTGACAGGTGAGGGGCACTAGGAGACCTCCAAGGGAGG AACAGCCCTCGCTGCACAGGTGTCCCTCTGATCTTTTGCGGTTCTACCCGATGGCTCCTTCAAGGAAGCT AGCCAGTAGATTGGAAGTGGGGGAGAGAGGGAGAGCCCTAGAGCCCTTGGGAGGACGGATTGACCTGGCC CGCCTCCCTTCCCACACTTTCCCGTCCCCGTTCCTTCTGCTTCCTTCAGTTTGTAAAGTTGGTGATTATA TTTTTGGGGGCTTTCTTTTTATTTTTTAAATGTAAAGTTTATTTATATTCCGTATTTAAAGTTGTAAAAA AAAAAAAGCCACAAAACAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC064056
Insert Size	726 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC064056, AAH64056
RefSeq Size	2629 bp
RefSeq ORF	726 bp
Locus ID	18591
Gene Summary	Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.[UniProtKB/Swiss-Prot Function]

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