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Sparc (NM_009242) Mouse Untagged Clone
CAT＃: MC207404
Sparc (untagged) - Mouse secreted acidic cysteine rich glycoprotein (Sparc), (10ug)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BM-40; ON |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207404 representing NM_009242
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCCTGGCAGCCCCTCAGCAGACTGAAG TTGCTGAGGAGATAGTGGAGGAGGAAACCGTGGTGGAGGAGACAGGGGTACCTGTGGGTGCCAACCCAGT CCAGGTGGAAATGGGAGAATTTGAGGACGGTGCAGAGGAAACGGTCGAGGAGGTGGTGGCTGACAACCCC TGCCAGAACCATCATTGCAAACATGGCAAGGTGTGTGAGCTGGACGAGAGCAACACCCCCATGTGTGTGT GCCAGGACCCCACCAGCTGCCCTGCTCCCATTGGCGAGTTTGAGAAGGTATGCAGCAATGACAACAAGAC CTTCGACTCTTCCTGCCACTTCTTTGCCACCAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAGCTC CACCTGGACTACATCGGACCATGCAAATACATCGCCCCCTGCCTGGATTCCGAGCTGACCGAATTCCCTC TGCGCATGCGTGACTGGCTCAAAAATGTCCTGGTCACCTTGTACGAGAGAGATGAGGGCAACAACCTCCT CACTGAGAAGCAGAAGCTGCGTGTGAAGAAGATCCATGAGAATGAGAAGCGCCTGGAGGCTGGAGACCAC CCCGTGGAGCTGTTGGCCCGAGACTTTGAGAAGAACTACAATATGTACATCTTCCCTGTCCACTGGCAGT TTGGCCAGCTGGATCAGCACCCTATTGATGGGTACCTGTCCCACACTGAGCTGGCCCCACTGCGTGCTCC CCTCATCCCCATGGAACATTGCACCACACGTTTCTTTGAGACCTGTGACCTAGACAACGACAAGTACATT GCCCTGGAGGAATGGGCCGGCTGCTTTGGCATCAAGGAGCAGGACATCAACAAGGATCTGGTGATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009242 |
| Insert Size | 909 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC004638, AAH04638 |
| RefSeq Size | 2641 bp |
| RefSeq ORF | 909 bp |
| Locus ID | 20692 |
| UniProt ID | P07214 |
| Gene Summary | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR204230 | Sparc (Myc-DDK-tagged) - Mouse secreted acidic cysteine rich glycoprotein (Sparc) |
CNY 3,600.00 |
|
| MR204230L3 | Lenti ORF clone of Sparc (Myc-DDK-tagged) - Mouse secreted acidic cysteine rich glycoprotein (Sparc) |
CNY 6,000.00 |
|
| MR204230L4 | Lenti ORF clone of Sparc (mGFP-tagged) - Mouse secreted acidic cysteine rich glycoprotein (Sparc) |
CNY 6,000.00 |
