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Psap (NM_011179) Mouse Untagged Clone
CAT＃: MC218954
Psap (untagged) - Mouse prosaposin (Psap), transcript variant 2, (10ug)
CNY 7,130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI037048; SGP; SGP-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC218954 representing NM_011179
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGCCCTCGCCCTCTTCGCCAGCCTTCTGGCCACCGCTCTGACCAGCCCTGTCCAAGACCCGAAGA CATGCTCTGGGGGCTCAGCAGTGCTGTGCAGAGATGTGAAGACGGCGGTGGACTGTGGGGCCGTGAAGCA CTGCCAGCAGATGGTCTGGAGCAAGCCCACAGCGAAATCCCTTCCTTGCGACATATGCAAAACTGTTGTC ACCGAAGCTGGGAACTTGCTGAAAGATAATGCTACGCAGGAGGAGATCCTTCATTACCTGGAGAAGACCT GTGAGTGGATTCATGACTCCAGCCTGTCGGCCTCGTGCAAGGAGGTGGTTGACTCTTACCTGCCTGTCAT CCTGGACATGATTAAGGGCGAGATGAGCAACCCTGGGGAAGTGTGCTCTGCGCTCAACCTCTGCCAGTCC CTTCAGGAGTACTTGGCCGAGCAAAACCAGAAACAGCTTGAGTCCAACAAGATCCCGGAGGTGGACATGG CCCGTGTGGTTGCCCCCTTCATGTCCAACATCCCTCTCCTGCTGTACCCTCAGGATCACCCCCGCAGCCA GCCCCAACCTAAGGCTAACGAGGACGTCTGCCAGGACTGTATGAAGCTGGTGTCTGATGTCCAGACTGCT GTGAAGACCAACTCCAGCTTTATCCAGGGCTTCGTGGACCACGTGAAGGAGGATTGTGACCGCTTGGGGC CAGGCGTGTCTGACATATGCAAGAACTACGTGGACCAGTATTCCGAGGTCTGTGTCCAGATGTTGATGCA CATGCAGGATCAGCAACCCAAGGAAATCTGTGTGCTGGCTGGCTTCTGTAATGAGGTCAAGAGAGTGCCA ATGAAGACTCTGGTCCCTGCCACCGAGACCATTAAGAACATCCTCCCTGCCCTGGAGATGATGGACCCCT ATGAGCAGAATCTGGTCCAGGCCCACAATGTGATTTTATGCCAGACCTGTCAGTTTGTGATGAATAAGTT TTCTGAGCTGATTGTCAATAATGCCACTGAGGAGCTCCTAGTTAAAGGTTTGAGCAACGCATGCGCACTG CTCCCCGATCCTGCCAGAACCAAGTGCCAGGAGGTGGTGGGAACATTTGGCCCCTCCCTGTTGGACATCT TTATCCATGAGGTAAACCCCAGCTCTCTGTGCGGTGTGATCGGCCTCTGTGCTGCCCGCCCGGAGTTGGT GGAGGCACTTGAGCAGCCTGCGCCAGCCATTGTATCTGCACTGCTCAAAGAGCCCACACCGCCAAAGCAG CCCGCACAGCCCAAGCAGTCGGCATTGCCCGCCCATGTGCCTCCTCAGAAGAATGGTGGGTTCTGTGAGG TGTGCAAGAAACTGGTCCTCTATTTGGAACATAACCTGGAGAAAAACAGCACCAAGGAGGAAATCCTGGC CGCACTTGAGAAGGGCTGCAGCTTCCTGCCAGACCCTTACCAGAAGCAGTGCGATGACTTTGTGGCTGAG TATGAGCCCTTGCTATTGGAGATCCTCGTGGAAGTGATGGATCCTGGATTTGTGTGCTCGAAAATTGGAG TTTGCCCTTCTGCCTATAAGCTGCTGCTGGGAACCGAGAAGTGTGTCTGGGGCCCTAGCTACTGGTGTCA GAACATGGAGACTGCCGCCCGATGCAATGCTGTCGATCATTGCAAACGCCATGTGTGGAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011179 |
| Insert Size | 1674 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011179.3, NP_035309.3 |
| RefSeq Size | 2676 bp |
| RefSeq ORF | 1674 bp |
| Locus ID | 19156 |
| UniProt ID | Q61207 |
| Gene Summary | This gene encodes a multifunctional glycoprotein that plays a role in the intracellular metabolism of various sphingolipids or secreted into the plasma, milk or cerebrospinal fluid. The encoded protein undergoes proteolytic processing to generate four different polypeptides known as saposin A, B, C or D, that are required for the hydrolysis of certain sphingolipids by lysosomal hydrolases. Alternately, the encoded protein is secreted into body fluids where it exhibits neurotrophic and myelinotrophic activities. A complete lack of the encoded protein is fatal to mice either at the neonatal stage or within the first month due to severe leukodystrophy and sphingolipid accumulation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature saposins. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (B). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227313 | Psap (tGFP-tagged) - Mouse prosaposin (Psap) transcript variant 2, (10ug) |
CNY 6,080.00 |
|
| MR227313 | Psap (Myc-DDK-tagged) - Mouse prosaposin (Psap), transcript variant 2 |
CNY 6,624.00 |
|
| MR227313L3 | Lenti ORF clone of Psap (Myc-DDK-tagged) - Mouse prosaposin (Psap), transcript variant 2 |
CNY 7,410.00 |
|
| MR227313L4 | Lenti ORF clone of Psap (mGFP-tagged) - Mouse prosaposin (Psap), transcript variant 2 |
CNY 7,410.00 |
