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Gria2 (NM_013540) Mouse Untagged Clone
CAT＃: MC222367
Gria2 (untagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (Gria2), transcript variant 2, (10ug)
CNY 9,712.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Glu; GluA2; GluR; Glur-2; GluR-B; gluR-K2; Glur2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222367 representing NM_013540
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAAGATTATGCATATTTCTGTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTT CTAACAGCATACAGATAGGGGGGCTATTTCCAAGGGGCGCTGATCAAGAATACAGTGCATTTCGGGTAGG GATGGTTCAGTTTTCCACTTCGGAGTTCAGACTGACACCCCATATCGACAATTTGGAGGTAGCCAACAGT TTCGCAGTCACCAATGCTTTCTGCTCCCAGTTTTCAAGAGGCGTCTATGCGATTTTTGGGTTTTACGACA AGAAGTCTGTAAATACCATCACATCATTCTGTGGGACACTGCATGTATCCTTCATCACACCAAGCTTCCC AACAGATGGCACGCATCCATTTGTCATCCAGATGCGACCTGACCTCAAAGGAGCACTCCTTAGCTTGATT GAGTACTACCAATGGGATAAGTTCGCATACCTCTATGACAGTGACAGAGGCTTATCAACACTGCAAGCTG TGCTGGATTCTGCTGCGGAGAAGAAGTGGCAGGTGACTGCTATCAATGTGGGGAACATTAACAATGACAA GAAAGATGAGACCTACAGATCACTCTTTCAAGATCTGGAGTTAAAAAAAGAACGGCGTGTAATCCTTGAC TGCGAAAGGGATAAAGTCAATGACATTGTGGACCAGGTTATTACCATTGGAAAGCATGTTAAAGGGTACC ATTATATCATTGCAAATCTGGGATTTACTGATGGAGACCTGCTGAAAATTCAGTTTGGAGGAGCAAATGT CTCTGGATTTCAGATTGTAGACTACGACGACTCCCTGGTGTCTAAATTTATAGAAAGATGGTCAACACTC GAAGAGAAAGAATACCCTGGAGCACACACAGCGACAATTAAGTATACTTCGGCCCTGACTTATGATGCTG TCCAAGTGATGACTGAAGCATTCCGCAATCTTCGGAAGCAGAGGATTGAAATCTCCAGGAGAGGAAATGC AGGAGATTGTTTGGCCAACCCAGCTGTGCCTTGGGGACAAGGCGTGGAAATAGAAAGGGCCCTCAAGCAG GTTCAAGTTGAAGGTCTCTCTGGAAATATAAAATTTGACCAGAACGGAAAACGAATAAACTACACAATTA ACATCATGGAGCTCAAAACAAATGGACCCCGGAAGATTGGGTACTGGAGTGAAGTGGATAAAATGGTTGT CACCCTAACCGAGCTCCCCTCTGGAAATGACACATCTGGGCTTGAAAACAAAACTGTGGTTGTCACCACA ATATTGGAATCTCCATATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGGAATGAGCGTTATGAGG GCTACTGTGTTGACTTAGCTGCAGAAATTGCCAAACATTGTGGATTCAAGTACAAGCTGACTATTGTTGG GGATGGCAAGTATGGGGCCAGGGATGCAGACACCAAAATTTGGAATGGTATGGTTGGAGAACTTGTATAT GGGAAAGCTGATATTGCCATTGCTCCATTAACTATCACTCTCGTGAGAGAAGAGGTGATTGACTTCTCGA AGCCATTCATGAGCCTTGGAATCTCTATCATGATCAAGAAGCCTCAGAAGTCCAAACCAGGAGTGTTTTC CTTTCTTGATCCTTTAGCCTATGAGATCTGGATGTGCATTGTGTTTGCCTACATTGGGGTCAGTGTAGTT TTATTCCTGGTCAGCAGATTTAGCCCCTACGAGTGGCACACTGAGGAATTTGAAGATGGAAGAGAAACAC AAAGTAGTGAATCAACTAATGAATTTGGGATTTTTAATAGTCTCTGGTTTTCCTTGGGTGCCTTTATGCG GCAAGGATGCGATATTTCGCCAAGATCTCTCTCTGGGCGCATTGTTGGAGGTGTGTGGTGGTTCTTTACC CTCATCATCATCTCCTCCTACACGGCTAACTTAGCTGCCTTCCTGACTGTAGAGAGGATGGTGTCGCCCA TCGAAAGTGCTGAGGATCTGTCTAAGCAAACAGAAATTGCTTATGGAACATTAGACTCTGGCTCCACTAA AGAGTTTTTCAGGAGATCTAAAATTGCAGTGTTTGATAAAATGTGGACTTATATGAGGAGTGCAGAGCCC TCTGTGTTTGTGAGGACTACGGCAGAAGGAGTAGCCAGAGTCAGGAAATCCAAAGGGAAGTATGCCTACT TGCTGGAGTCCACAATGAATGAGTACATCGAGCAGAGGAAGCCTTGCGACACCATGAAAGTGGGCGGCAA CCTGGATTCCAAAGGCTACGGCATCGCCACACCTAAAGGATCCTCATTAAGAAATGCGGTTAACCTCGCA GTACTAAAACTGAATGAACAAGGCCTGTTGGACAAATTGAAAAACAAATGGTGGTACGACAAAGGAGAGT GCGGCAGCGGGGGAGGTGATTCCAAGGAAAAGACCAGTGCCCTCAGTCTGAGCAACGTTGCTGGAGTATT CTACATCCTTGTCGGGGGCCTTGGTTTGGCAATGCTGGTGGCTTTGATTGAGTTCTGTTACAAGTCAAGG GCCGAGGCGAAACGAATGAAGGTGGCAAAGAATGCACAGAATATTAACCCATCTTCCTCGCAGAATTCCC AGAATTTTGCAACTTATAAGGAAGGTTACAACGTATATGGCATCGAGAGTGTTAAAATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013540 |
| Insert Size | 2652 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013540.2, NP_038568.2 |
| RefSeq Size | 6841 bp |
| RefSeq ORF | 2652 bp |
| Locus ID | 14800 |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate exon in the 3' coding region compared to transcript variant 1, and encodes an isoform (2, also known as the flop isoform) that is the same length as isoform 1, but with few amino acid differences. RNA editing (CAG->CGG) changes Gln607Arg. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227065 | Gria2 (tGFP-tagged) - Mouse glutamate receptor ionotropic AMPA2 (alpha 2) (Gria2) transcript variant 2, (10ug) |
CNY 9,690.00 |
|
| MR227065 | Gria2 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (Gria2), transcript variant 2 |
CNY 9,696.00 |
|
| MR227065L3 | Lenti ORF clone of Gria2 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (Gria2), transcript variant 2 |
CNY 10,740.00 |
|
| MR227065L4 | Lenti ORF clone of Gria2 (mGFP-tagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (Gria2), transcript variant 2 |
CNY 10,740.00 |
