Hdac7 (NM_001204279) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5830434K02Rik; HD7; HD7a; Hdac7a; mFLJ00062
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229117 representing NM_001204279 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTGCGGGTGGGCCAGCGGCCCACGGTGGAGCCCCCACCAGAGCCTGCGCTGCTGACCCTGCAAC ACCCCCAACGCCTGCACCGCCATCTCTTCCTGGCAGGCTTACACCAGCAACAGCGCTCAGCCGAGCCCAT GAGGCTCTCCATGGACCCACCAATGCCGGAGCTGCAGGGGGGACAGCAGGAGCAAGAACTTCGGCAACTT CTCAATAAAGACAAGAGCAAGCGAAGTGCCGTAGCCAGCAGTGTGGTCAAGCAGAAGCTGGCTGAAGTGA TCCTGAAGAAACAGCAGGCAGCCCTTGAGAGAACAGTCCATCCCAGCAGCCCCAGTATTCCCTACAGCCT GCCCACTGAACCCCCGGAACACTTTCCCTTGCGTAAAACAGTGTCTGAACCCAACCTGAAGTTGCGCTAC AAACCCAAGAAATCCCTGGAGAGACGCAAGAATCCCCTGCTCAGGAAGGAGAGTGCCCCGCCCAGCCTTC GGAGGAGGCCTGCCGAGACCCTTGGAGATTCCTCCCCCAGTAGTAGCAGCACACCCGCGTCAGGGTGCAG CTCCCCTAATGACAGCGAGCATGGCCCTAACCCTGCCCTAGGCTCAGAGGCTGATGGTGACCGCAGGACC CATTCAACTTTAGGCCCTCGGGGTCCTGTACTGGGGAACCCCCATGCTCCCCTCTTCCTGCACCACGGTC TGGAGCCAGAGGCTGGGGGCACCTTACCCTCTCGCCTGCAACCCATTCTCCTGCTGGACCCCTCAGTCTC TCATGCCCCACTGTGGACTGTGCCTGGCCTTGGGCCCTTGCCCTTCCACTTTGCCCAGCCCTTACTGACC ACCGAGCGGCTCTCTGGGTCAGGCCTCCATCGACCACTTAACCGGACCCGCTCAGAGCCCCTGCCCCCCA GCGCCACAGCCTCCCCTCTGCTGGCCCCCCTGCAGCCCCGCCAGGATCGGCTCAAACCTCACGTCCAGCT GATCAAGCCAGCCATCTCCCCTCCCCAGAGGCCTGCCAAGCCCAGTGAGAAGCCCCGACTGCGACAGATA CCCTCGGCTGAGGACCTAGAGACAGATGGTGGGGGAGTGGGACCTATGGCGAATGATGGCCTGGAACATA GGGAGTCAGGCCGTGGGCCTCCTGAGGGCAGAGGCTCCATTTCTCTGCAGCAGCATCAACAGGTGCCACC CTGGGAGCAGCAGCATCTAGCCGGGCGGCTCTCTCAGGGAAGCCCCGGGGACTCCGTGCTGATACCTCTG GCCCAGGTTGGACACCGGCCCCTGTCCAGAACCCAGTCTTCCCCAGCAGCACCTGTCTCCCTGCTGAGCC CAGAGCCCACCTGTCAGACCCAAGTCCTCAACAGCTCAGAGACACCTGCTACAGGGCTGGTCTATGACTC GGTGATGCTGAAACACCAATGTTCCTGTGGAGACAACAGCAAGCATCCCGAGCATGCAGGCCGCATCCAG AGCATCTGGTCCCGGCTGCAGGAACGGGGTCTCCGCAGCCAGTGTGAGTGTCTCCGAGGCCGAAAGGCTT CCCTAGAGGAGCTGCAGTCAGTCCACTCTGAACGGCACGTGCTCCTCTACGGCACGAACCCACTCAGCCG CCTCAAACTGGATAACGGGAAGCTTACAGGACTCCTGGCACAGCGGACGTTTGTGATGCTACCCTGTGGC GGGGTTGGGGTCGATACTGACACCATCTGGAACGAGCTGCATTCCTCCAATGCAGCCCGCTGGGCTGCGG GCAGTGTCACCGACCTTGCCTTCAAAGTAGCTTCCCGAGAGCTGAAGAATGGCTTTGCTGTGGTGCGACC CCCGGGACACCATGCAGATCATTCTACAGCCATGGGCTTCTGCTTCTTCAACTCCGTGGCCATCGCCTGC CGACAGCTACAGCAACACGGCAAAGCCAGCAAGATCCTCATTGTTGACTGGGATGTTCACCATGGCAACG GCACACAGCAGACTTTCTACCAGGACCCCAGTGTGCTCTACATTTCCCTTCATCGCCATGACGACGGCAA CTTCTTCCCAGGCAGTGGGGCCGTGGATGAGGTGGGAACTGGCAGTGGCGAGGGCTTCAATGTCAACGTG GCTTGGGCTGGGGGCTTGGATCCACCCATGGGGGATCCTGAGTACCTGGCTGCTTTCAGGATAGTGGTGA TGCCCATTGCCCGAGAGTTTGCTCCAGACCTGGTCCTGGTGTCTGCTGGGTTTGATGCTGCGGAGGGTCA CCCAGCCCCGCTGGGTGGCTACCATGTTTCTGCCAAATGTTTTGGGTACATGACGCAGCAGTTGATGAAC TTGGCAGGAGGCGCCGTGGTGTTGGCCTTAGAGGGTGGACATGACCTCACGGCCATCTGTGATGCCTCGG AGGCCTGTGTAGCTGCTCTTCTGGGCAACAAGGTGGACCCCCTTTCAGAAGAAAGCTGGAAACAGAAACC CAACCTCAGTGCCATCCGCTCGCTGGAAGCTGTGGTCAGGGTGCACAGGAAATACTGGGGCTGCATGCAG CGCTTGGCCTCCTGTCCAGACTCCTGGCTACCCAGAGTGCCGGGAGCTGATGCAGAAGTGGAAGCCGTGA CCGCGCTGGCATCCCTTTCTGTGGGCATCCTGGCTGAAGACAGGCCCTCGGAGCGGCTGGTGGAAGAGGA AGAACCCATGAACCTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204279
Insert Size	2679 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204279.1, NP_001191208.1
RefSeq Size	4156 bp
RefSeq ORF	2679 bp
Locus ID	56233
Gene Summary	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Positively regulates the transcriptional repressor activity of FOXP3 (By similarity). Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding (By similarity). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) lacks a coding exon and uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (6) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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