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Thtpa (NM_153083) Mouse Tagged ORF Clone
CAT＃: MG202596
- TrueORF®
Thtpa (tGFP-tagged) - Mouse thiamine triphosphatase (Thtpa)
ORF Plasmid: DDK
"NM_153083" in other vectors (3)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW122966; ThTP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202596 representing NM_153083
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGGGCTTGATTGAAGTGGAGCGAAAGTTCGCTCCAGGGCCTGACACGGAGGAAAGGCTGCAGG AGTTGGGGGCCACCCTGGAGCACCGGGTCACCTTCCGAGACACATACTATGATACCTCTGAGTTAAGCCT TATGCTGTCTGACCACTGGCTGCGACAACGAGAAGGTAGTGGATGGGAGCTCAAATGTCCTGGAGTAACA GGTGTCTCAGGACCTCACAATGAGTATGTGGAAGTCACATCGGAAGCTGCGATTGTGGCCCAGCTCTTTG AACTGCTCGGGTCTGGGGAGCAGAAGCCTGCAGGTGTGGCTGCTGTCCTGGGCTCGCTGAAGCTGCAGGA GGTGGCTAGTTTTATTACTACGCGAAGCTCCTGGAAGCTGGCCTTATCTGGAGCCCATGGACAGGAGCCT CAGCTCACAATAGACTTGGATTCAGCGGACTTCGGCTATGCTGTGGGTGAGGTAGAGGCCATGGTGCACG AGAAGGCTGAAGTCCCGGCTGCCCTAGAGAAGATCATCACCGTCAGTAGCATGCTTGGAGTGCCAGCACA GGAGGAGGCACCAGCCAAGCTCATGGTATACCTACAGCGCTTCAGGCCTCTGGACTATCAGCGCCTGCTA GAAGCAGCCAGTTCTGGTGAGGCCACAGGGGACTCAGCATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202596 representing NM_153083
Red=Cloning site Green=Tags(s) MAQGLIEVERKFAPGPDTEERLQELGATLEHRVTFRDTYYDTSELSLMLSDHWLRQREGSGWELKCPGVT GVSGPHNEYVEVTSEAAIVAQLFELLGSGEQKPAGVAAVLGSLKLQEVASFITTRSSWKLALSGAHGQEP QLTIDLDSADFGYAVGEVEAMVHEKAEVPAALEKIITVSSMLGVPAQEEAPAKLMVYLQRFRPLDYQRLL EAASSGEATGDSAS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153083 |
| ORF Size | 672 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153083.5 |
| RefSeq Size | 2824 bp |
| RefSeq ORF | 675 bp |
| Locus ID | 105663 |
| UniProt ID | Q8JZL3 |
| Gene Summary | This gene encodes a cytosolic enzyme that catalyzes the conversion of thiamine triphosphate to thiamine diphosphate. The encoded protein requires divalent cations for enzymatic activity. The gene product is believed to play a role in regulation of thiamine triphosphate intracellular concentration, and it may be a messenger in cell signaling in response to cellular stress. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR202596 | Thtpa (Myc-DDK-tagged) - Mouse thiamine triphosphatase (Thtpa) |
CNY 1,416.00 |
|
| MR202596L3 | Lenti ORF clone of Thtpa (Myc-DDK-tagged) - Mouse thiamine triphosphatase (Thtpa) |
CNY 4,750.00 |
|
| MR202596L4 | Lenti ORF clone of Thtpa (mGFP-tagged) - Mouse thiamine triphosphatase (Thtpa) |
CNY 4,750.00 |
