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Scnm1 (NM_027013) Mouse Tagged ORF Clone
CAT＃: MG202752
- TrueORF®
Scnm1 (tGFP-tagged) - Mouse sodium channel modifier 1 (Scnm1)
ORF Plasmid: DDK
"NM_027013" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3110001I17Rik; Scnm1-ps |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202752 representing NM_027013
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTTAAGAGGGAAGGGGACGACTGGAGTCAACTCAATGTGCTCAAAAAACGGAGAGTTGGGGACC TGCTGGCTAGTTACATCCCTGAGGACGAGGCACTGATGCTGCGGGATGGACGCTTTGCTTGTGCCATCTG CCCCCATCGACCAGTACTAGACACGCTGGCCATGTTGACAGCCCACCGTGCAGGCAAGAAGCATTTGTCC AGTCTGAAGCTTTTCTATGGCAAAAAGCAAACAGGCAAGGGAACAGAGCAAAATCCAAGACAGCAGAACG AATTGAAGACAGAAAGCAAAACTGAGGCTCCTTTGCTAACCCAGACTCGAATCATCACCCAGAATGCTCT ACACAGAGCTCCCCACTATAACAGTTGCTGCCGGAGGAAGCACAGACCAGAAGCCCCTGCTCCCTCTGTC TCCAGTCCTCCTTTGCCAACTGCAGAGGTCCAACTCCAGAGTGCAGAGATCAGTAAGGAACCTGAGCCTA GGGAGAGATCAGATGCCAAAGAGTCAGCAGCTCTCTTGGCCTCTGCACCCATGAGCCCCACCAAACGACG AGTCCTGAACCATTACCTTACCCTCCGAAGCTCTGGATGGGTCCCAGATGGACGAGGTCGATGGATAAAG GATGAAAATGTTGAGTTTGACTCTGATGAGGAAGAGCCCCCCGATCTCCCCTTGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202752 representing NM_027013
Red=Cloning site Green=Tags(s) MSFKREGDDWSQLNVLKKRRVGDLLASYIPEDEALMLRDGRFACAICPHRPVLDTLAMLTAHRAGKKHLS SLKLFYGKKQTGKGTEQNPRQQNELKTESKTEAPLLTQTRIITQNALHRAPHYNSCCRRKHRPEAPAPSV SSPPLPTAEVQLQSAEISKEPEPRERSDAKESAALLASAPMSPTKRRVLNHYLTLRSSGWVPDGRGRWIK DENVEFDSDEEEPPDLPLD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027013 |
| ORF Size | 687 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027013.1, NP_081289.1 |
| RefSeq Size | 871 bp |
| RefSeq ORF | 561 bp |
| Locus ID | 69269 |
| UniProt ID | Q8K136 |
| Gene Summary | Mutations in the voltage-gated sodium channel gene Scn8a lead to neurological problems in mice. For one particular mutation, Scn8amedJ, mice live to adulthood but have tremors and muscle weakness, among other problems, in all strains except those derived from C57BL6 mice. In these strains, the product of the Scnm1 gene (229 aa) partially overcomes the effects of the Scn8amedJ mutation. However, in C57BL6-derived mice, a one nt change in the penultimate exon creates a premature stop codon, truncating the Scnm1 protein at 186 aa. This truncated protein lacks the ability to overcome the effects of the Scn8amedJ mutation, and these mice suffer paralysis and juvenile death. [provided by RefSeq, Jul 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207680 | Scnm1 (untagged) - Mouse sodium channel modifier 1 (Scnm1), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MR202752 | Scnm1 (Myc-DDK-tagged) - Mouse sodium channel modifier 1 (Scnm1), transcript variant 1 |
CNY 2,400.00 |
|
| MR202752L3 | Lenti ORF clone of Scnm1 (Myc-DDK-tagged) - Mouse sodium channel modifier 1 (Scnm1), transcript variant 1 |
CNY 4,750.00 |
|
| MR202752L4 | Lenti ORF clone of Scnm1 (mGFP-tagged) - Mouse sodium channel modifier 1 (Scnm1), transcript variant 1 |
CNY 4,750.00 |
