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Akr1b3 (NM_009658) Mouse Tagged ORF Clone
CAT＃: MG204522
- TrueORF®
Akr1b3 (tGFP-tagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3)
ORF Plasmid: DDK
"NM_009658" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ahr-1; Ahr1; Akr1b1; Aldor1; Aldr1; ALR2; AR |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204522 representing NM_009658
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCCATCTGGAACTCAACAACGGCACCAAGATGCCCACCCTGGGCCTGGGCACCTGGAAGTCCC CTCCTGGCCAGGTGACTGAGGCCGTGAAAGTTGCTATTGACTTGGGGTACCGCCACATTGACTGCGCCCA GGTGTACCAGAATGAGAAGGAGGTGGGAGTGGCCCTCCAGGAGAAGCTCAAGGAGCAGGTGGTGAAGCGG CAGGATCTCTTCATTGTCAGCAAGCTGTGGTGCACGTTCCATGACAAGAGCATGGTGAAAGGAGCCTTCC AGAAGACACTGAGCGACCTGCAGCTGGACTACCTGGATCTCTACCTTATTCACTGGCCAACGGGGTTCAA GCCTGGGCCCGACTATTTCCCACTGGATGCCTCAGGGAACGTGATACCTAGTGACACCGATTTTGTGGAC ACTTGGACGGCTATGGAACAACTAGTGGATGAAGGTTTGGTGAAAACAATCGGTGTCTCCAACTTCAACC CTCTTCAGATTGAGAGGATCTTGAACAAACCTGGCTTAAAATATAAGCCTGCGGTGAACCAGATCGAGTG CCACCCGTACCTAACTCAGGAGAAGCTGATTGAATACTGCCACAGCAAAGGCATCGTGGTGACAGCATAT AGTCCCCTTGGTTCTCCTGACAGGCCCTGGGCCAAGCCTGAAGATCCGTCTCTCCTGGAGGATCCCAGGA TCAAAGCAATTGCAGCCAAGTACAATAAAACTACAGCCCAGGTGCTGATCCGGTTCCCCATTCAGAGGAA CTTGGTAGTGATCCCCAAGTCGGTGACACCAGTGCGCATTGCTGAGAACTTGAAGGTCTTTGACTTTGAG CTGAGCAGCGAGGACATGGCCACTCTACTCAGCTACAACAGGAACTGGAGGGTGTGCGCCTTGATGAGCT GTGCCAAACACAAGGATTACCCCTTCCACGCCGAAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204522 representing NM_009658
Red=Cloning site Green=Tags(s) MASHLELNNGTKMPTLGLGTWKSPPGQVTEAVKVAIDLGYRHIDCAQVYQNEKEVGVALQEKLKEQVVKR QDLFIVSKLWCTFHDKSMVKGAFQKTLSDLQLDYLDLYLIHWPTGFKPGPDYFPLDASGNVIPSDTDFVD TWTAMEQLVDEGLVKTIGVSNFNPLQIERILNKPGLKYKPAVNQIECHPYLTQEKLIEYCHSKGIVVTAY SPLGSPDRPWAKPEDPSLLEDPRIKAIAAKYNKTTAQVLIRFPIQRNLVVIPKSVTPVRIAENLKVFDFE LSSEDMATLLSYNRNWRVCALMSCAKHKDYPFHAEV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009658 |
| ORF Size | 948 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009658.2, NP_033788.2 |
| RefSeq Size | 1402 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 11677 |
| UniProt ID | P45376 |
| Gene Summary | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed:17381426, PubMed:19010934, PubMed:7851421). Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins (PubMed:19010934). Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203763 | Akr1b3 (untagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3), (10ug) |
CNY 2,000.00 |
|
| MR204522 | Akr1b3 (Myc-DDK-tagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3) |
CNY 2,081.00 |
|
| MR204522L3 | Lenti ORF clone of Akr1b3 (Myc-DDK-tagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3) |
CNY 4,750.00 |
|
| MR204522L4 | Lenti ORF clone of Akr1b3 (mGFP-tagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3) |
CNY 4,750.00 |
