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Rdh10 (NM_133832) Mouse Tagged ORF Clone
CAT＃: MG205116
- TrueORF®
Rdh10 (tGFP-tagged) - Mouse retinol dehydrogenase 10 (all-trans) (Rdh10)
ORF Plasmid: DDK
"NM_133832" in other vectors (4)
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CNY 3,710.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3110069K09Rik; 4921506A21Rik; AI875664; AW549993; D1Ertd762e; m366Asp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205116 representing NM_133832
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATCGTAGTGGAGTTCTTCGTGGTCACTTTCAAAGTGCTGTGGGCGTTCGTGCTGGCCGCGGCGC GCTGGCTGGTGCGGCCCAAGGAGAAGAGCGTGGCCGGCCAGGTGTGCCTCATCACGGGCGCGGGCAGCGG CCTGGGCCGCCTCTTTGCTCTCGAGTTCGCCCGGCGCCGGGCGCTGCTGGTTCTCTGGGACATCAACACG CAGAGCAATGAGGAGACCGCAGGCATGGTTCGCCACATCTACCGGGACCTGGAGGCGGCCGACGCTGCTG CTCTGCAAGCTGGAAAAGGTGAGGAGGAAATCCTGCCCCCGTGTAACTTACAGGTTTTTACCTACACTTG TGACGTGGGGAAGAGAGAGAATGTCTACCTGACAGCTGAAAGGGTCCGCAAGGAGGTTGGCGAGGTCTCG GTCCTGGTCAATAATGCTGGTGTGGTTTCTGGACATCACCTTCTGGAATGTCCTGATGAGCTCATTGAAA GAACCATGATGGTCAACTGCCACGCACACTTCTGGACCACTAAGGCCTTTCTTCCAACGATGCTAGAGAT TAATCATGGCCACATTGTGACGGTCGCGAGCTCCCTGGGACTGTTCAGCACTGCTGGAGTTGAGGATTAC TGTGCCAGTAAATTTGGAGTTGTGGGTTTTCACGAGTCTCTGAGCCATGAGCTAAAGGCTGCTGAAAAGG ATGGAATTAAAACAACTCTGGTTTGCCCTTACCTTGTAGACACGGGCATGTTCAGAGGCTGCCGAATCAG AAAAGAAATTGAGCCCTTTTTGCCCCCCTTGAAGCCTGATTACTGTGTGAAGCAGGCTATGAGGGCCATC CTCACTGACCAGCCCATGGTCTGCACGCCCCGCCTCATGTACATCGTGACATTCATGAAGAGCATCCTTC CTTTTGAAGCCGTCGTGTGCATGTATCGATTTCTAGGAGCGGACAAGTGTATGTATCCCTTTATTGCTCA AAGAAAACAAGCCACAAACAACAATGAAGCAAAAAATGGAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205116 representing NM_133832
Red=Cloning site Green=Tags(s) MNIVVEFFVVTFKVLWAFVLAAARWLVRPKEKSVAGQVCLITGAGSGLGRLFALEFARRRALLVLWDINT QSNEETAGMVRHIYRDLEAADAAALQAGKGEEEILPPCNLQVFTYTCDVGKRENVYLTAERVRKEVGEVS VLVNNAGVVSGHHLLECPDELIERTMMVNCHAHFWTTKAFLPTMLEINHGHIVTVASSLGLFSTAGVEDY CASKFGVVGFHESLSHELKAAEKDGIKTTLVCPYLVDTGMFRGCRIRKEIEPFLPPLKPDYCVKQAMRAI LTDQPMVCTPRLMYIVTFMKSILPFEAVVCMYRFLGADKCMYPFIAQRKQATNNNEAKNGI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133832 |
| ORF Size | 1023 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133832.2 |
| RefSeq Size | 2295 bp |
| RefSeq ORF | 1026 bp |
| Locus ID | 98711 |
| UniProt ID | Q8VCH7 |
| Gene Summary | Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinol to all-trans-retinal. Has no detectable activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol (By similarity). Required for normal embryonic development.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Light-driven Regeneration of Cone Visual Pigments through a Mechanism Involving RGR opsin in Müller glial cells
,null,
Neuron
,PubMed ID 31056353
[Rdh10]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204456 | Rdh10 (untagged) - Mouse retinol dehydrogenase 10 (all-trans) (Rdh10), (10ug) |
CNY 2,000.00 |
|
| MR205116 | Rdh10 (Myc-DDK-tagged) - Mouse retinol dehydrogenase 10 (all-trans) (Rdh10) |
CNY 3,330.00 |
|
| MR205116L3 | Lenti ORF clone of Rdh10 (Myc-DDK-tagged) - Mouse retinol dehydrogenase 10 (all-trans) (Rdh10) |
CNY 5,230.00 |
|
| MR205116L4 | Lenti ORF clone of Rdh10 (mGFP-tagged) - Mouse retinol dehydrogenase 10 (all-trans) (Rdh10) |
CNY 5,230.00 |
