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Agk (NM_023538) Mouse Tagged ORF Clone
CAT＃: MG206703
- TrueORF®
Agk (tGFP-tagged) - Mouse acylglycerol kinase (Agk)
ORF Plasmid: DDK
"NM_023538" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610037M15Rik; 6720408I04Rik; AI465370; Mulk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206703 representing NM_023538
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGCATTCTTTAAAACGCTTCGAAATCATTGGAAGAAAACTACAGCTGGGCTCTGTTTGCTGACAT GGGGAGGTCACTGGCTCTACGGGAAGCACTGTGATAACCTGCTAAGAAGAGCAGCCTGTCAAGAAGCTCA GGTGTTTGGCAACCAGCTCATTCCTCCCAATGCACAAGTGAAGAAAGCCACCGTCTTTCTCAATCCCGCA GCTTGCAAAGGCAAAGCCAGAACTCTATTTGAAAAAAATGCTGCTCCAATTTTACACCTGTCTGGCATGG ATGTGACTGTTGTCAAGACGGATTATGAGGGACAAGCCAAGAAGCTCTTGGAGTTGATGGAAAGCACTGA TGTGATCATTGTTGCTGGAGGAGACGGCACACTGCAGGAGGTTGTTACTGGAGTACTGAGAAGAACAGAT GAGGCTACCTTCAGTAAGATTCCCATTGGATTTATCCCGCTGGGACAGACCAGTAGTTTGAGTCACACTC TCTTTGCTGAAAGTGGAAACAAAGTCCAACATATTACAGACGCCACACTTGCCATTGTGAAAGGAGAGAC TGTTCCACTTGATGTCTTGCAGATCAAGGGTGAAAAAGAACAACCTGTGTATGCAATGACTGGCCTCCGG TGGGGATCTTTCAGAGATGCTGGCGTCAAAGTTAGCAAGTACTGGTATCTTGGTCCACTAAAAACCAAAG CAGCCCACTTTTTCAGCACTCTTCAGGAATGGCCTCAGACTCATCAAGCCTCCATCTCTTACACGGGACC TAGAGAGAGACCTCCCATTGAGCCTGAAGAGACTCCTCCCAGGCCGTCTCTGTATAGGAGAATATTACGC AGGCTTGCCTCATTCTGGGCACAGCCACAGGATGCCTCCTCCCGAGAGGTAAGCCCTGAGGTCTGGAAAG ATGTACAACTGTCCACCATTGAACTGTCCATCACAACCCGAAACACCCAACTTGACCTGATGAGCAAAGA GGATTTTATGAACATTTGCATTGAACCTGACACTGTCAGCAAAGGAGATTTCATAATCATAGGAAGTAAG AAGGTGAGAGACCCTGGCCTGCGTGCAGCTGGCACCGAGTGCCTCCAAGCCAGCCACTGTACTCTGGTTC TTCCTGAGGGAACTGAGGGCTCCTTCAGCATTGACAGCGAGGAGTACGAAGCCATGCCGGTGGAGGTCAA ACTACTGCCCAGAAAGCTGCGGTTCTTCTGTGATCCGAGGAAGAGAGAGCAAATGCTGCCGAGCACATCC CAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206703 representing NM_023538
Red=Cloning site Green=Tags(s) MTAFFKTLRNHWKKTTAGLCLLTWGGHWLYGKHCDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFLNPA ACKGKARTLFEKNAAPILHLSGMDVTVVKTDYEGQAKKLLELMESTDVIIVAGGDGTLQEVVTGVLRRTD EATFSKIPIGFIPLGQTSSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVYAMTGLR WGSFRDAGVKVSKYWYLGPLKTKAAHFFSTLQEWPQTHQASISYTGPRERPPIEPEETPPRPSLYRRILR RLASFWAQPQDASSREVSPEVWKDVQLSTIELSITTRNTQLDLMSKEDFMNICIEPDTVSKGDFIIIGSK KVRDPGLRAAGTECLQASHCTLVLPEGTEGSFSIDSEEYEAMPVEVKLLPRKLRFFCDPRKREQMLPSTS Q TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023538 |
| ORF Size | 1263 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023538.2 |
| RefSeq Size | 2236 bp |
| RefSeq ORF | 1266 bp |
| Locus ID | 69923 |
| UniProt ID | Q9ESW4 |
| Gene Summary | Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed:15252046). Does not phosphorylate sphingosine (PubMed:15252046). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (By similarity). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (By similarity). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (By similarity). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202948 | Agk (untagged) - Mouse acylglycerol kinase (Agk), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MR206703 | Agk (Myc-DDK-tagged) - Mouse acylglycerol kinase (Agk), nuclear gene encoding mitochondrial protein |
CNY 3,330.00 |
|
| MR206703L3 | Lenti ORF clone of Agk (Myc-DDK-tagged) - Mouse acylglycerol kinase (Agk), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
|
| MR206703L4 | Lenti ORF clone of Agk (mGFP-tagged) - Mouse acylglycerol kinase (Agk), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
