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Prodh2 (NM_019546) Mouse Tagged ORF Clone
CAT＃: MG207277
- TrueORF®
Prodh2 (tGFP-tagged) - Mouse proline dehydrogenase (oxidase) 2 (Prodh2)
ORF Plasmid: DDK
"NM_019546" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2510028N04Rik; 2510038B11Rik; MmPOX; MmPOX1; POX1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207277 representing NM_019546
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTGGACGCGTCTGCCGCTGTATGGCCCCTCCAAGCCCTCTACAGGTGGCTGGCAGCCCCTGCGCT TTGATGGTGGGGCCTTCCATGTCAAGGGAACCGCAGAACTGGCACGGGCTTTGCTAGTGCTTCGCCTATG CGCCTGGCCCCCTTTGGTCACTCATGGACTAGCGTTTCAGGCCTGGTCTCAGCGACTCCTGGGATCCCGG CTCTCAGGCGCACTTCTTCGAGCATCCATCTACGGGCAGTTTGTGGCCGGGGAGACAGCAGAGGAAGTGA GGAACTGTGTCGGGCAGCTGCAGGCCCTGGGACTCCAGCCCCTGTTGGCAGTACCCACCGAGGAGGAACC AGACTCCACTGCCAAGACCAGTGAGGTCTGGTATGAGGAGAACCTTAGCGCCATGCTGCGCTGTGTGGAC TTATCCCGAGCCCTCGTGGACGCCCACGGCCCAGCCAGGAACAGCCTCATGCAGCTGAAGGTGACCGCGC TAGCCAGCACTCGGCTTTGTAAAGAGCTGTCGGCTTGGATCCAAAGACCCAGAGGCTCCTCGGAGCTGAG CCCTGAGAGGCTGGCAGAAGCCATGGACTCGGGTCGGAACCTCCAGCTCTCCTGCCTCAGCACAGAACAG AATCAGCACCTGCAGGCCTCCCTCAGCCGCTTGCACCGAGTGGCACAGCACGCCCGGGCGAAGTGTGTGC GGCTGCTGGTAGATGCTGAATATACTTTCATTAACCCTGCACTGTCCCTGCTGGTGGCTGCCCTGGCTGT GCGCTGGAACAGCCCTGAGGAAGGTGGTCCGTGGGTGTGGAACACTTACCAGGCCTATCTAAAGGACACT CACCAACGGCTGGAGCAGGACGCCGAGGCAGCACACAAGGCTGGCCTGGCATTTGGGGTGAAGTTGGTGC GAGGTGCCTATCTGGACAAGGAGAGATCCATGACACAGCTCCAAGGGAAGGAAGACTGTACCCAGCCTGA CTATGAGGCCACTAGTCGGAGTTACAGCCGCTGTCTAGAGCTGATGCTGCGCTGCGTGTCGAACCACGGT CCCCCGTGTCACCTCATGGTGGCTTCCCACAATGAAGAATCCGTTCGCCAGGCAACTAAGCGCATGTGGG AGCTGGGCATTCCTCTGGATGGGCCTGTCTGTTTTGGACAACTTCTGGGCATGTGTGACCATGTCTCCCT GGCATTAGGGCAGGCTGGATATATGGTGTACAAGTCTATTCCCTATGGCTGCCTGGAGGAGGTGATTCCC TACCTGATCCGAAGAGCCCAGGAGAACAGGAGTGTGCTGCAGGGTGCCCGCAGGGAGCAGGCACTACTCA GCCAGGAACTGTGGCGGAGACTGCTGGGAAGGACGGCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207277 representing NM_019546
Red=Cloning site Green=Tags(s) MIWTRLPLYGPSKPSTGGWQPLRFDGGAFHVKGTAELARALLVLRLCAWPPLVTHGLAFQAWSQRLLGSR LSGALLRASIYGQFVAGETAEEVRNCVGQLQALGLQPLLAVPTEEEPDSTAKTSEVWYEENLSAMLRCVD LSRALVDAHGPARNSLMQLKVTALASTRLCKELSAWIQRPRGSSELSPERLAEAMDSGRNLQLSCLSTEQ NQHLQASLSRLHRVAQHARAKCVRLLVDAEYTFINPALSLLVAALAVRWNSPEEGGPWVWNTYQAYLKDT HQRLEQDAEAAHKAGLAFGVKLVRGAYLDKERSMTQLQGKEDCTQPDYEATSRSYSRCLELMLRCVSNHG PPCHLMVASHNEESVRQATKRMWELGIPLDGPVCFGQLLGMCDHVSLALGQAGYMVYKSIPYGCLEEVIP YLIRRAQENRSVLQGARREQALLSQELWRRLLGRTA SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019546 |
| ORF Size | 1368 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019546.5 |
| RefSeq Size | 1818 bp |
| RefSeq ORF | 1371 bp |
| Locus ID | 56189 |
| UniProt ID | Q8VCZ9 |
| Gene Summary | Dehydrogenase that converts trans-4-L-hydroxyproline to delta-1-pyrroline-3-hydroxy-5-carboxylate (Hyp) using ubiquinone-10 as the terminal electron acceptor. Can also use proline as a substrate but with a very much lower efficiency. Does not react with other diastereomers of Hyp: trans-4-D-hydroxyproline and cis-4-L-hydroxyproline. Ubiquininone analogs such as menadione, duroquinone and ubiquinone-1 react more efficiently than oxygen as the terminal electron acceptor during catalysis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202880 | Prodh2 (untagged) - Mouse proline dehydrogenase (oxidase) 2 (Prodh2), (10ug) |
CNY 2,000.00 |
|
| MR207277 | Prodh2 (Myc-DDK-tagged) - Mouse proline dehydrogenase (oxidase) 2 (Prodh2) |
CNY 3,330.00 |
|
| MR207277L3 | Lenti ORF clone of Prodh2 (Myc-DDK-tagged) - Mouse proline dehydrogenase (oxidase) 2 (Prodh2) |
CNY 5,230.00 |
|
| MR207277L4 | Lenti ORF clone of Prodh2 (mGFP-tagged) - Mouse proline dehydrogenase (oxidase) 2 (Prodh2) |
CNY 5,230.00 |
