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Chrm1 (NM_007698) Mouse Tagged ORF Clone
CAT＃: MG207339
- TrueORF®
Chrm1 (tGFP-tagged) - Mouse cholinergic receptor, muscarinic 1, CNS (Chrm1)
"NM_007698" in other vectors (5)
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CNY 4,470.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Chrm-1; M1; M1R |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207339 representing NM_007698
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACCTCAGTGCCCCCTGCTGTCAGTCCCAACATCACCGTCTTGGCACCAGGAAAGGGTCCCTGGC AAGTGGCATTCATCGGGATCACCACAGGCCTCCTGTCTCTGGCTACAGTGACAGGCAACCTGCTGGTGCT CATCTCCTTCAAGGTCAACACAGAGCTCAAGACAGTCAACAACTACTTCCTGCTGAGCCTGGCCTGTGCT GATCTCATCATTGGCACTTTCTCCATGAACCTCTATACCACATACCTGCTCATGGGCCACTGGGCTCTGG GCACACTGGCCTGTGACCTCTGGCTGGCCCTGGACTATGTGGCCAGCAACGCCTCTGTCATGAATCTTCT GCTCATCAGCTTTGACCGTTACTTCTCAGTGACCCGACCCCTGAGCTACCGAGCCAAGCGCACTCCCCGC AGGGCAGCTCTGATGATTGGCCTCGCGTGGTTGGTTTCCTTCGTTCTCTGGGCCCCAGCCATCCTCTTCT GGCAATACCTAGTTGGGGAGCGGACAGTGCTGGCTGGGCAGTGCTACATCCAGTTCCTCTCCCAACCCAT CATCACTTTTGGCACAGCCATGGCCGCCTTCTACCTCCCTGTCACAGTCATGTGTACGCTGTACTGGCGC ATCTACCGGGAGACAGAAAACCGAGCCCGGGAGCTAGCAGCCCTACAGGGCTCTGAGACACCAGGCAAAG GTGGTGGCAGCAGCAGCAGCTCAGAGAGGTCACAGCCAGGAGCTGAAGGCTCACCCGAGTCACCTCCAGG CCGCTGCTGTCGCTGTTGCCGGGCACCCAGACTTCTGCAGGCCTACAGCTGGAAAGAAGAAGAGGAAGAG GATGAAGGCTCCATGGAGTCCCTCACATCCTCTGAAGGTGAGGAGCCTGGCTCAGAAGTGGTGATCAAGA TGCCTATGGTAGATCCTGAGGCACAGGCACCCACCAAGCAGCCTCCCAAAAGCTCCCCAAATACAGTCAA GAGGCCCACCAAGAAAGGCCGAGACCGAGGCGGCAAAGGCCAAAAACCCCGAGGGAAGGAACAACTGGCC AAGAGAAAGACCTTCTCACTGGTCAAGGAGAAGAAGGCAGCTCGGACCCTGAGTGCCATCCTGCTGGCCT TCATCCTCACCTGGACACCATATAACATCATGGTGCTGGTGTCTACATTCTGCAAGGACTGTGTTCCAGA AACCCTATGGGAGCTGGGCTACTGGCTTTGCTACGTCAACAGCACTGTCAACCCCATGTGCTACGCACTC TGCAACAAAGCCTTCCGGGACACTTTCCGCCTGCTGTTGCTCTGCCGCTGGGACAAGCGGCGCTGGCGCA AAATCCCCAAGCGCCCTGGCTCTGTGCACCGCACCCCCTCCCGCCAATGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207339 representing NM_007698
Red=Cloning site Green=Tags(s) MNTSVPPAVSPNITVLAPGKGPWQVAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACA DLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPR RAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWR IYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPESPPGRCCRCCRAPRLLQAYSWKEEEEE DEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPKSSPNTVKRPTKKGRDRGGKGQKPRGKEQLA KRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTVNPMCYAL CNKAFRDTFRLLLLCRWDKRRWRKIPKRPGSVHRTPSRQC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007698 |
| ORF Size | 6206 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007698.3, NP_031724.2 |
| RefSeq Size | 3816 bp |
| RefSeq ORF | 1383 bp |
| Locus ID | 12669 |
| UniProt ID | P12657 |
| Gene Summary | The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216104 | Chrm1 (untagged) - Mouse cholinergic receptor, muscarinic 1, CNS (Chrm1), transcript variant 2, (10ug) |
CNY 5,990.00 |
|
| MG221834 | Chrm1 (tGFP-tagged) - Mouse cholinergic receptor muscarinic 1 CNS (Chrm1) transcript variant 2, (10ug) |
CNY 4,470.00 |
|
| MR221834 | Chrm1 (Myc-DDK-tagged) - Mouse cholinergic receptor, muscarinic 1, CNS (Chrm1), transcript variant 2 |
CNY 3,656.00 |
|
| MR221834L3 | Lenti ORF clone of Chrm1 (Myc-DDK-tagged) - Mouse cholinergic receptor, muscarinic 1, CNS (Chrm1), transcript variant 2 |
CNY 5,990.00 |
|
| MR221834L4 | Lenti ORF clone of Chrm1 (mGFP-tagged) - Mouse cholinergic receptor, muscarinic 1, CNS (Chrm1), transcript variant 2 |
CNY 5,990.00 |
