Pccb (NM_025835) Mouse Tagged ORF Clone
CAT#: MG208651
- TrueORF®
Pccb (tGFP-tagged) - Mouse propionyl Coenzyme A carboxylase, beta polypeptide (Pccb)
ORF Plasmid: DDK
"NM_025835" in other vectors (4)
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CNY 7,640.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1300012P06Rik; AI314687; R74805 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208651 representing NM_025835
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGATTAGGATTCGGGCGGTGGCGGCTGGAGCGAGGCTCAGCGTTCTGAATTGCGGCCTCG GCATCACGACCCGCGGCCTTTGCAGCCAGCCGGTTTCAGTTAAAGAGCGCATCGACAACAAGCGCCATGC AGCGTTGCTGGGAGGAGGCCAGCGCCGCATCGACGCGCAGCACAAGCGAGGGAAGCTAACAGCCAGGGAG CGGATCAGTCTTCTGCTGGATCCTGGCAGCTTCATGGAGAGCGACATGTTTGTGGAACACAGATGTGCCG ACTTCGGAATGGCTGCTGATAAGAATAAGTTTCCCGGAGATAGTGTGGTCACTGGACGGGGCCGAATCAA TGGACGATTGGTTTATGTCTTCAGTCAGGATTTTACAGTTTTTGGAGGCAGTCTGTCAGGAGCACATGCT CAAAAGATCTGCAAAATCATGGACCAGGCCATAACAGTGGGGGCTCCAGTGATCGGGCTCAATGACTCCG GGGGCGCCAGAATCCAGGAGGGGGTGGAGTCCTTGGCTGGCTACGCGGACATCTTCCTGAGGAATGTCAC AGCGTCTGGAGTCATCCCGCAGATTTCTCTGATCATGGGCCCGTGTGCTGGTGGGGCCGTCTACTCCCCT GCCCTAACAGACTTCACATTCATGGTGAAGGACACATCTTACCTGTTTATCACTGGCCCTGAAGTTGTGA AGTCTGTTACCAATGAGGATGTCACTCAAGAGCAGCTGGGTGGTGCCAAGACCCACACCACTGTGTCAGG TGTGGCCCATAGAGCTTTCGACAATGATGTTGATGCTCTGTGTAACCTGCGTGAATTCTTCAATTTCCTG CCCCTCAGCAGCCAGGACCCGGCTCCCATCCGAGAGTGCCATGACCCCAGTGACCGTCTGGTTCCTGAGC TGGACACAGTTGTCCCTTTGGAGTCAAGCAAAGCCTACAACATGCTGGACATCATCCACGCAGTGATTGA CGAGAGAGAGTTTTTTGAGATAATGCCCAGTTACGCAAAAAATATTGTCGTTGGCTTCGCAAGAATGAAT GGGAGGACCGTTGGAATTGTCGGCAACCAGCCCAATGTGGCCTCAGGGTGCTTGGACATTAATTCATCCG TGAAGGGGGCTCGTTTTGTCAGATTCTGTGACGCATTCAACATTCCCCTCATCACTTTCGTTGACGTCCC TGGCTTCCTGCCTGGCACGGCGCAGGAGTATGGGGGCATCATCAGGCATGGTGCCAAGCTGCTCTACGCG TTCGCCGAGGCAACTGTGCCCAAAATCACAGTCATCACCAGGAAGGCCTATGGAGGTGCCTATGATGTCA TGAGCTCCAAACACCTTCTTGGTGACACCAACTATGCCTGGCCCACAGCTGAGATTGCGGTCATGGGAGC AAAGGGTGCTGTGGAGATCATCTTCAAAGGACACCAAGATGTGGAAGCCGCCCAGGCAGAGTATGTGGAG AAGTTCGCCAACCCTTTCCCAGCAGCCGTGAGAGGGTTTGTGGATGACATCATCCAGCCATCCTCTACTC GTGCTCGGATATGCTGTGACCTGGAAGTCCTGGCCAGCAAGAAGGTCCATCGTCCCTGGAGGAAACATGC AAATATCCCACTG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208651 representing NM_025835
Red=Cloning site Green=Tags(s) MAAAIRIRAVAAGARLSVLNCGLGITTRGLCSQPVSVKERIDNKRHAALLGGGQRRIDAQHKRGKLTARE RISLLLDPGSFMESDMFVEHRCADFGMAADKNKFPGDSVVTGRGRINGRLVYVFSQDFTVFGGSLSGAHA QKICKIMDQAITVGAPVIGLNDSGGARIQEGVESLAGYADIFLRNVTASGVIPQISLIMGPCAGGAVYSP ALTDFTFMVKDTSYLFITGPEVVKSVTNEDVTQEQLGGAKTHTTVSGVAHRAFDNDVDALCNLREFFNFL PLSSQDPAPIRECHDPSDRLVPELDTVVPLESSKAYNMLDIIHAVIDEREFFEIMPSYAKNIVVGFARMN GRTVGIVGNQPNVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYA FAEATVPKITVITRKAYGGAYDVMSSKHLLGDTNYAWPTAEIAVMGAKGAVEIIFKGHQDVEAAQAEYVE KFANPFPAAVRGFVDDIIQPSSTRARICCDLEVLASKKVHRPWRKHANIPL SGPTRTRRLE - GFP Tag - V |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025835 |
ORF Size | 1623 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025835.3 |
RefSeq Size | 2291 bp |
RefSeq ORF | 1626 bp |
Locus ID | 66904 |
UniProt ID | Q99MN9 |
Gene Summary | This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205562 | Pccb (untagged) - Mouse propionyl Coenzyme A carboxylase, beta polypeptide (Pccb), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
MR208651 | Pccb (Myc-DDK-tagged) - Mouse propionyl Coenzyme A carboxylase, beta polypeptide (Pccb), nuclear gene encoding mitochondrial protein |
CNY 6,040.00 |
|
MR208651L3 | Lenti ORF clone of Pccb (Myc-DDK-tagged) - Mouse propionyl Coenzyme A carboxylase, beta polypeptide (Pccb), nuclear gene encoding mitochondrial protein |
CNY 6,650.00 |
|
MR208651L4 | Lenti ORF clone of Pccb (mGFP-tagged) - Mouse propionyl Coenzyme A carboxylase, beta polypeptide (Pccb), nuclear gene encoding mitochondrial protein |
CNY 6,650.00 |