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Mad1l1 (NM_010752) Mouse Tagged ORF Clone
CAT＃: MG210250
- TrueORF®
Mad1l1 (tGFP-tagged) - Mouse mitotic arrest deficient 1-like 1 (Mad1l1)
ORF Plasmid: DDK
"NM_010752" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI173502; AW550425; Mad1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210250 representing NM_010752
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGACCTCGGGGAGAACACCACAGTCCTCTCCTCCCTGAGATCTCTGAACAACTTCATTTCTCAGC GAATGGAGGGAACATCAGGCCTGGATGTGTCCACGTCTGCCTCAGGGTCCCTACAGAAGCAGTATGAGTA TCACATGCAGCTGGAGGAAAGAGCAGAGCAGATCCGCTCAAAGTCCTACCTCATCCAAGTGGAGCGGGAG AAGATGCAGATGGAGCTGAGCCACAAGAGAGCCCGAGTGGAGCTGGAGAGAGCAGCCAGCACCAATGCCA GGAACTACGAGCGCGAGGTAGATCGCAACCAGGAGCTCCTGGCACGCATTCGACAGCTCCAGGAGTGTGA GGCCACAGCAGAAGAGAAGATGCGGGAGCAGCTGGAGCGCCACAGGCTGTGCAAGCAAAACCTGGATGCT GTCAGTCAGCAGCTGCGGGAACAGGAGGACAGCCTGGCTTCGGCCAGGGAGATGATTAGTTCATTGAAGG GGCGTGTCTCGGAGCTGCAGTTGAGTGCCATGGACCAGAAGGTACAGGTCAAGCGGCTGGAGTCTGAAAA GCAGGAGCTGAAGGAGCAGCTGGAGCTGCAGCAGCGGAAATGGCAAGAGGCAAATCAGAAGATCCAGGAG CTGCAGGCCAGTCAAGATGAGAGAGCTGAGCATGAGCAGAAGATTAAGGACCTGGAGCAGAAGCTGTGTC TACAAGAGCAGGATGCAGCTGTGGTGAAGAGCATGAAGTCTGAGCTGATGCGGATGCCCAGGATGGAGCG TGAGCTGAAGAGGCTGCATGAAGAGAACACTCATCTAAGGGAGATGAAGGAGACCAATGGGCTGCTCACA GAGGAGCTGGAAGGTCTGCAGAGGAAACTGAGCCGGCAGGAGAAGATGCAGGAGGCTCTGGTTGACCTGG AGTTGGAAAAGGAGAAGCTACTTGCAAAGCTGCAGAGCTGGGAGAACCTGGACCAGACCATGGGCTTGAA TCTCAGGACTCCAGAGGACCTTTCTAGGTTCGTGGTTGAGCTACAGCAGCGAGAGCTCACCCTGAAGGAG AAGAATAACAGCATCACCAGCAGTGCCCGGGGTCTGGAGAAGGTCCAGCAGCAGCTCCAGGATGAGGTCC GGCAGGCCAATGCACAGCTGCTGGAGGAGCGGAAAAAGAGGGAGACACATGAGGCACTTGCCCGAAGGCT CCAGAAGCGCAATGCATTGCTGACCAAGGAGAGAGATGGCATGCGTGCCATCCTGGGATCCTACGACAGT GAGCTGACCCAAACTGAGTACTCAACCCAGCTGACACAGCGCCTGTGGGAGGCCGAGGACATGGTTCAGA AGGTACATGCCCACAGCTCGGAGATGGAGGCTCAGCTGTCTCAGGCCCTGGAGGAGCTTGGGGTTCAGAA GCAGAGAGCTGACACGCTGGAGATGGAGTTGAAGATGCTGAAGGCCCAGACAAGCTCAGCTGAATCTAGC TTCTCATTCTGCAAAGAGGAGGTGGATGCACTCAGGTTGAAGGTGGAGGAGCTGGAGGGTGAGCGGAGCC GTCTGGAACAGGAAAAGCAGGTGCTGGAGATGCAGATGGAGAAACTAACCCTACAGGGTGACTACAACCA GAGTCGAACCAAAGTGCTACACATGAGCCTTAACCCAATCAGCATGGCCAGGCAACGCCAGCATGAGGAC CATGACCGGCTGCAGGAGGAATGTGAGCGCTTACGGGGACTTGTGCATGCCCTGGAGAGAGGGGGCCCCA TCCCTGCTGACCTGGAGGCTGCCTCCAGTCTGCCCTCATCAAAGGAGGTGGCAGAGCTGCGGAAGCAGGT GGAAAGTGCTGAGCTGAAGAACCAGCGGCTGAAGGAGGTTTTCCAGACCAAGATCCAGGAGTTCCGCAAA GTTTGCTACACGCTGACCGGCTACCAGATTGACGTGACCACGGAGAGCCAATACCGCCTCACCTCCCGAT ACGCTGAGCACCAGACGGACTGCCTCATCTTCAAGGCCACAGGACCCTCGGGCTCCAAGATGCAGCTGCT GGAGACAGAGTTCTCACGGTCTGTGCCTGAGCTCATTGAACTGCACTTGCTGCAGCAGGACAGCATCCCT GCCTTCCTCAGTGCGCTCACCATAGAGCTGTTCAGCCGCCAGACCTCTATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210250 representing NM_010752
Red=Cloning site Green=Tags(s) MEDLGENTTVLSSLRSLNNFISQRMEGTSGLDVSTSASGSLQKQYEYHMQLEERAEQIRSKSYLIQVERE KMQMELSHKRARVELERAASTNARNYEREVDRNQELLARIRQLQECEATAEEKMREQLERHRLCKQNLDA VSQQLREQEDSLASAREMISSLKGRVSELQLSAMDQKVQVKRLESEKQELKEQLELQQRKWQEANQKIQE LQASQDERAEHEQKIKDLEQKLCLQEQDAAVVKSMKSELMRMPRMERELKRLHEENTHLREMKETNGLLT EELEGLQRKLSRQEKMQEALVDLELEKEKLLAKLQSWENLDQTMGLNLRTPEDLSRFVVELQQRELTLKE KNNSITSSARGLEKVQQQLQDEVRQANAQLLEERKKRETHEALARRLQKRNALLTKERDGMRAILGSYDS ELTQTEYSTQLTQRLWEAEDMVQKVHAHSSEMEAQLSQALEELGVQKQRADTLEMELKMLKAQTSSAESS FSFCKEEVDALRLKVEELEGERSRLEQEKQVLEMQMEKLTLQGDYNQSRTKVLHMSLNPISMARQRQHED HDRLQEECERLRGLVHALERGGPIPADLEAASSLPSSKEVAELRKQVESAELKNQRLKEVFQTKIQEFRK VCYTLTGYQIDVTTESQYRLTSRYAEHQTDCLIFKATGPSGSKMQLLETEFSRSVPELIELHLLQQDSIP AFLSALTIELFSRQTSI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010752 |
| ORF Size | 2151 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010752.3, NP_034882.2 |
| RefSeq Size | 2640 bp |
| RefSeq ORF | 2154 bp |
| Locus ID | 17120 |
| UniProt ID | Q9WTX8 |
| Gene Summary | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200677 | Mad1l1 (untagged) - Mouse mitotic arrest deficient 1-like 1 (Mad1l1), (10ug) |
CNY 2,000.00 |
|
| MR210250 | Mad1l1 (Myc-DDK-tagged) - Mouse mitotic arrest deficient 1-like 1 (Mad1l1) |
CNY 5,256.00 |
|
| MR210250L3 | Lenti ORF clone of Mad1l1 (Myc-DDK-tagged) - Mouse mitotic arrest deficient 1-like 1 (Mad1l1) |
CNY 7,700.00 |
|
| MR210250L4 | Lenti ORF clone of Mad1l1 (mGFP-tagged) - Mouse mitotic arrest deficient 1-like 1 (Mad1l1) |
CNY 7,700.00 |
