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Polr3b (NM_027423) Mouse Tagged ORF Clone
CAT＃: MG211682
- TrueORF®
Polr3b (tGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide B (Polr3b)
ORF Plasmid: DDK
"NM_027423" in other vectors (3)
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CNY 10,830.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2700078H01Rik; A330032P03Rik; C85372; RPC2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211682 representing NM_027423
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTTCTAGCCGAGGAGTTCGGGAGCCTGACTCCGGAGCAGCTGACTGCGCCGATCCCGACTGTGG AGGAAAAATGGAGGCTGCTTCCAGCATTTTTAAAGGTTAAAGGCCTGGTGAAACAGCACATAGACTCCTT TAACTATTTCATTAATGTAGAGATAAAGAAGATAATGAAAGCCAATGAAAAGGTTACAAGTGATGCTGAC CCCATGTGGTACTTAAAATACCTTAATATCTATGTCGGACTTCCTGATGTTGAAGAAAGCTTCAATGTAA CTAGACCAGTGTCCCCTCATGAGTGCCGTCTGAGGGACATGACGTACTCCGCCCCAATCACAGTGGACAT TGAGTATACCCGAGGCAGCCAGAGGATAATCCGCAACGCCTTACCCATTGGCAGAATGCCCATCATGCTC CGAAGCTCGAACTGTGTTCTAACAGGGAAAACGCCGGCAGAGTTTGCCAAACTGAATGAGTGTCCACTAG ACCCAGGTGGCTACTTCATTGTTAAAGGAGTGGAAAAAGTCATTCTTATCCAAGAGCAGCTGTCCAAGAA CAGGATCATTGTGGAGGCCGACAGGAAAGGAGCCGTTGGAGCCTCAGTTACCAGCTCCACCCATGAGAAG AAAAGCAGAACCAACATGGCCGTGAAACAGGGTCGATTCTATCTGAGGCACAACACCCTGTCTGAAGATA TCCCCATTGTCATCATATTTAAGGCCATGGGTGTGGAGAGTGACCAGGAAATCGTGCAGATGATTGGAAC AGAGGAGCACGTGATGGCTGCATTTGGGCCCAGCTTGGAGGAGTGCCAGAAAGCTCAGATCTTCACCCAG ATGCAGGCATTGAAGTATATAGGGAATAAAGTAAGAAGACAAAGAATGTGGGGAGGTGGACCAAAGAAAA CCAAAATAGAAGAAGCAAGAGAACTCCTGGCTTCTACCATTCTGACCCACGTACCAGTGAAGGAATTCAA TTTCCGAGCTAAGTGTATCTACACTGCAGTGATGGTGCGAAGGGTGATCCTGGCCCAAGGAGATAACAAG GTCGATGACAGAGACTATTATGGCAACAAACGACTGGAATTGGCAGGACAGCTCTTATCTCTTCTTTTTG AAGATTTGTTTAAAAAGTTTAATTCAGAAATGAAGAAGATTGCAGACCAAGTGATTCCTAAACAGAGAGC AGCCCAGTTTGACGTCGTTAAACACATGCGCCAAGACCAGATTACCAACGGCATGGTCAATGCCATCTCC ACGGGAAATTGGTCTCTGAAGAGATTTAAGATGGACCGACAGGGTGTGACCCAGGTGCTGTCTCGCTTGT CATATATATCTGCACTCGGCATGATGACAAGAATCTCTTCCCAGTTTGAAAAGACGAGGAAAGTGAGTGG CCCTCGTTCTCTGCAGCCGTCTCAGTGGGGAATGCTCTGTCCCTCCGACACACCTGAAGGGGAGGCCTGT GGTTTGGTTAAGAACTTGGCCCTCATGACACATATAACAACTGACATGGAAGATGGACCCATTATTAAGT TAGCTGGGAACTTGGGAGTAGAAGATGTGAATTTATTATGTGGAGAAGAGCTCTCTTACCCAAATGTGTT TCTTGTCTTCCTGAATGGTAACATCCTTGGTGTCATTCGAGACCATAAAAAGCTTGTCAGCACATTCCGG CTGATGCGAAGAGCAGGCTATATCAATGAATTTGTTTCTATCTCCACAAACCTTACGGATCGCTGTGTTT ATATTTCCTCTGATGGCGGGCGCTTGTGCAGGCCTTACATAATTGTTAAGAAACAAAAGCCAGCTGTCAC AAATAAGCACATGGAAGAGTTGGCTCAAGGCTACAGGAATTTTGAAGATTTCTTACATGAAAGTCTAGTT GAATACTTAGATGTGAATGAAGAAAATGACTGTAACATTGCGCTCTACGAACATACAATTAATAAAGACA CCACCCACTTGGAGATTGAACCCTTCACTCTGCTTGGTGTTTGTGCCGGCCTGATCCCATACCCTCATCA CAACCAGTCTCCAAGAAACACCTACCAGTGTGCTATGGGCAAGCAAGCCATGGGTACCATTGGCTACAAT CAACGGAACAGAATTGATACACTTATGTATCTACTAGCATATCCACAAAAGCCCATGGTTAAAACAAAAA CAATCGAGTTGATAGATTTTGAGAAGTTGCCGGCTGGCCAGAACGCAACTGTTGCCGTGATGAGCTACAG CGGCTATGACATTGAGGACGCGCTGGTTTTGAACAAGGCCTCTCTGGACAGAGGTTTTGGGCGTTGTCTT GTGTATAAAAATGCTAAGTGCACCTTGAAACGATACACCAATCAGACCTTTGATAAGGTGATGGGTCCCA TGTTGGATGCTGCTACCAGAAAACCCATCTGGCGACATGAAATTTTAGATGCAGATGGCATTTGTTCTCC AGGTGAGAAAGTGGAAAACAAACAGGTGCTTGTAAACAAGTCCATGCCCACAGTGACACAGATTCCTTTG GAAGGGAGCAACGTGCCACAGCAACCACAGTATAAAGATGTGCCTATCACGTACAAAGGTGCGACAGACT CCTACATCGAGAAAGTGATGATCTCTTCCAACGCCGAGGATGCTTTCCTCATCAAGATGCTCCTGAGACA GACGCGGCGCCCAGAGATTGGAGACAAATTCAGCAGTCGCCATGGACAAAAGGGTGTCTGTGGCCTGATC GTCCCCCAGGAAGACATGCCGTTCTGTGATTCTGGCATCTGTCCCGACATCATAATGAACCCACACGGCT TCCCATCAAGAATGACGGTTGGAAAGTTAATTGAGCTGCTGGCTGGCAAGGCAGGCGTGTTAGACGGCAG GTTCCACTACGGCACTGCCTTTGGAGGGAGTAAAGTGAAGGATGTGTGTGAAGATCTCGTTCGCCATGGT TACAACTACCTGGGGAAGGACTACGTCACCTCAGGCATCACAGGTGAGCCCTTAGAAGCATACATCTATT TTGGACCTGTGTATTATCAGAAACTGAAACACATGGTGCTGGATAAAATGCACGCCCGGGCCCGAGGACC ACGAGCTGTCCTTACCAGGCAGCCCACTGAAGGGCGGTCGCGCGATGGTGGCTTGCGACTTGGAGAAATG GAACGCGACTGTCTAATCGGCTATGGAGCGAGTATGCTTTTGCTGGAGAGACTAATGATCTCAAGTGATG CCTTTGAGGTTGACGTCTGCGGGCAGTGTGGACTCCTGGGATATTCTGGCTGGTGCCACTACTGCAAATC TTCGTGCCACGTGTCCTCCCTCCGCATCCCGTATGCCTGCAAGCTGCTCTTCCAGGAGTTACAGTCCATG AACATCATCCCCAGGCTGAAACTGGCCAAGTACAACGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211682 representing NM_027423
Red=Cloning site Green=Tags(s) MDVLAEEFGSLTPEQLTAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDAD PMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIML RSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEK KSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQ MQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNK VDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAIS TGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEAC GLVKNLALMTHITTDMEDGPIIKLAGNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVSTFR LMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLV EYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYN QRNRIDTLMYLLAYPQKPMVKTKTIELIDFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCL VYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPL EGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLI VPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHG YNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEM ERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSM NIIPRLKLAKYNE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027423 |
| ORF Size | 3399 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027423.2 |
| RefSeq Size | 4858 bp |
| RefSeq ORF | 3402 bp |
| Locus ID | 70428 |
| UniProt ID | P59470 |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211682 | Polr3b (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide B (Polr3b) |
CNY 7,888.00 |
|
| MR211682L3 | Lenti ORF clone of Polr3b (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide B (Polr3b) |
CNY 11,780.00 |
|
| MR211682L4 | Lenti ORF clone of Polr3b (mGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide B (Polr3b) |
CNY 11,780.00 |
