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Tiam2 (NM_011878) Mouse Tagged ORF Clone
CAT＃: MG212067
- TrueORF®
Tiam2 (tGFP-tagged) - Mouse T-cell lymphoma invasion and metastasis 2 (Tiam2)
ORF Plasmid: DDK
"NM_011878" in other vectors (2)
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CNY 18,810.00
货期*
3周
规格
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经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3000002F19Rik; mKIAA2016; STEF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212067 representing NM_011878
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACTCCGAGAGTCAATATACCTTCCAAGGATCCAAGAATCATAGTAATACTGTCACTGGTGCTA AGCAAAAGCCTTGCTCTCTGAAAATACGCAGCGTTCATGCAAAAGACGAGAAGTCCTTACATGGTTGGAC TCATGGGAGCAGCGGTGCAGGCTACAAGTCCAGGTCCCTAGCCCGAAGCTGCCTTTCTCACTTTAAGAAT CACCAGCCTTACGCCACCAGACTCAGTGGACCCACATGTAAAGTCTCAAAGGGCACCACCTACTCTAAGC ACAGAGCAAATACCCCGGGAAATGATTTCCAGGGCAACAGTGGTGCTTTCTTACCTGAGAATGGCTTCCA CTATGTTGACCGCGAGTCAGAGGAAAGCCATATCACCTCCAATGGGCACCTTCTCACCTGCTATGGGAGA AAGGAAAGCCTCGCCTCCACTCCTCCAGGCGAGGACCACAGGAGCCCCAGGGTGCTCATCAAGACCCTGG GAAAGCTAGACGGGTGTTTAAGAGTCGAGTTCCACAACGGTGGCAACCCCCACAAGGGGACCTCCGAGGA CCCCAGTGGACCTGTACGGCTGCTGAGATACTCCCCTACCTTAGCATCGGAAACCTGCCCGGTGCGGGAA ACCAGGCGGCATTCCGCTGCAGGCTCCCCATCCAGCCAGCGGCCCTCTCCCACTGACTCTCGCCTGCGCT CCAGCAAAGGCAGCTCCCTGAGCTCAGAGTCATCCTGGTATGACTCCCCCTGGGGCAACGCTGGGGAGGT GAGCGAGGTGGAGGGCTCCTTCCTGGCTCCCAGCACTCCAGACCCCAGCCTCCCCAGCAGCTTCCCACCC AGTGACACCAAAAAGCCTTTCAACCAAAGCTCTTCCCTCTCCTCCCTCCGGGAATTGTACAAAGATCCCA ACCTGGGGTGCCGCTCACCTTCCGGCACCTGCCTTTCTTCCAATGAGTACATCAGCTCTCAAGTCAGCCT GAACAACCGAGTCTCCTTTGCGTCTGACATGGATGTGCCCTCCAGGGTGGATCACAGGGATCCCCTGCAC TATAGTTCCTTTACTCTCCCCTGTCGCAAGTCCAAAGCCTTAACTGAAGATGCAGCTAAGAAAGACACCC TCAAAGCCAGAATGCGGCGCTTCAGTGACTGGACAGGAAGCCTCTCCAGGAAGAAGAGGAAACTGCAGGA ACCCAGGTCCATGGAGGGCAGTGAGTACTTTGATAGCCACTCAGATGGACTGAATGCAGAAGGGCAGGTG CCCGCGCAGACATCTTCCTTACTGTGGTCAGGGGGCTCGGCTCAGACCCTGCCTCACAGAAGCGAATCCA CTCACGCTATCAGCGTCGATCCCCTCCGACAGAACATCTATGAGAATTTCATGCGAGAGCTCGAAATGAG CAGGAGCAACACAGAACACGTGGAAACTTCCACAGAGACCATGGAGTCCAGCAGCGAGTCTGTCAGCTCA CTGGAGCAGCTGGATCTGCTCTTTGAGAAGGAGCAGGGAGTGGTCCGGAAAGCCGGGTGGCTCTTCTTCA AACCCCTTGTCACCTTGCAGAAGGAGAGGAAACTGGAGCTGGTGGCTCGGAGGAAGTGGAAACAATACTG GGTGACCCTGAAAGGCTGTACTCTGCTGTTTTATGAGACCTATGGAAAGAATTCCACAGAGCAGAATAGT GCCCCACGGTGTGCCCTCTTTGCAGAGGACAGCATCGTGCAGTCTGTCCCAGAGCATCCCAAGAAGGAAC ACGTGTTCTGCCTGAGTAACTCCTGTGGGGACGTCTACCTATTCCAGGCCACTAGCCAGACAGATCTGGA AAACTGGGTCACAGCCATCCACTCGGCGTGCGCATCCCTCTTTGCAAAGAAGCACGGAAAGGAGGACACG GTGCGACTGCTAAAGAGCCAGACCAGAAGCCTGCTTCAGAAGATAGACATGGATAGCAAGATGAAGAAGA TGGCAGAGTTGCAGCTGTCTGTGGTGAGCGACCCCAAGAACAGGAAGGCCATCGAGAATCAGATCCGGCA ATGGGAGCAGAATCTGGAAAAATTCCACATGGACCTGTTCCGCATGCGCTGCTATTTGGCGAGCTTACAA GGTGGGGAGTTACCAAATCCCAAGAGTCTCCTTGCTGCCACCAGCCGCCCCTCCAAGCTGGCTCTTGGCA GGCTGGGCGTCCTGTCTGTTTCGTCTTTCCATGCTCTGGTGTGTTCCAGAGATGATTCCACTCTCAGGAA AAGAACACTTTCCCTTACCCAGAGAGGAAAAAGCAAGAAAGGCATATTTTCTTCATTGAAAGGTCTGGAC ACCCTAGCAAGAAAGGGTAGGGAGAAGAGAGCTTCCATAACTCAGATGTTTGATTCTAGCCACAGCCATG GATTTCTTGGAACTCAGCTACCTCAAAAGTCCACTAACTCCAACAAGGCCCATGACCTGCATCTGTATGG CTCCGCAGTAGACAGCGCGCTGCGAGACAGCATGTGGGAAGTCCAGACTTATGTCCACTTCCAGGATAAC GAAGGAGTTACTGTGACCATCAAGCCAGAGCACAGGGTGGAAGATGTTCTGGCTTTGGTGTGCAAGATGA GACAGTTGGAACCCACTCACTATGGTCTTCAGCTCCGAAAGGTGGTCGATAAAAGTGTGGAGTGGTGTGT GCCCGCGCTGTATGAATACATGCAAGAGCAGGTTTATGATGAAATCGAAGTTTTCCCACTCAGTGTGTAT GACGTGCAGCTAACCAAGACTGGGGACATGACTGACTTTGGGTTTGCAGTCACAGTTCAGGTGGACGAAC ACCAGCATCTCAACCGGATATTTATCAGTGATGTTCTCCCTGACAGCCTGGCATACGGAGGAGGGCTGAG AAAGGGCAACGAAATCACAAGCTTAAATGGGGAACCAGTGTCTGACCTTGACATCCAGCAGATGGAGGCT TTGTTTTCTGAGAAGAGCGTTGGCCTCACTCTAGTCGCCCGGCCTGTGACCACAAGACGAACCCTGTGTG CTTCCTGGTCAGACAGTGACCTGTTCTCCAGGGACCAGAAAAGTCTGCCGCCCTCTCCCAACCAGTCCCA GCTGCTGGAGGAATTCCTGGATAACTTTAGAAAAACCGCCACGAGTGATTTCAGCAATGTCCCTGAGATC ACAACTGGCTTGAAGAGGAGCCAGACAGAAGGCACCCTGGATCAAGTGCCCCACAGGGAAAAGATGGAGC AGACATTCCTGAGCGCTGACCAGATTGCGGAGCTCTGCAGGGACTTGAACAACACCCACACCAACAGTAT GGAAGCACCAACAGAGAGCCATGACCCACCTCCCAGGCCTCTGGCTCGTCACCTCTCAGATGCAGATCGC CTCCGGAAAGTCATCCAGGAGCTTGTAGACACAGAGAAGTCTTACGTGAAGGATCTGAGCTGCCTCTTTG AACTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATGGAGTCACTTTTTGGGAG CCTGCCAGAGATGCTGGAGTTTCAAAAGGTGTTCCTGGAGACGTTGGAGGATGCGATCTCCGCTTCCTCG GACTTTAGTGTCCTGGAAACCCCCTCACAGTTTCGAAAATTGCTGTTCTCCCTTGGAGGTTCTTTCCTCT ACTATGCGGATCACTTTAAGCTATACAGTGGGTTCTGTGCCAACCACATTAAAGTACAGAGGGTTCTAGA GCGAGCTAAAACGGACAAGGCCTTCAAGGCTTTTCTGGATGCCCGAAATCCCACCAAGCAGCACTCCTCC ACGCTGGAGTCCTATCTCATCAAGCCTGTTCAGAGAGTGCTCAAGTATCCTCTGCTTCTCAAGGAGCTAG TGTCACTGACTGACCATGAGAGTGAAGAACACTATCACCTGACAGAAGCACTAAAGGCCATGGAAAAAGT CGCCAGTCACATCAATGAGATGCAGAAGATCTACGAGGATTACGGGATGGTGTTTGACCAGCTGGTGGCA GAGCAGAGTGGCACAGAGAAGGAAGTGACAGAGCTGTCCATGGGGGAACTTCTGATGCACTCTACAGTTT CCTGGTTGAATCCGTTCCTGTCTCTAGGAAAAGCCAGGAAGGACATTGAGCTCACAGTATTTGTTTTTAA GAGAGCTGTCATACTGGTTTATAAAGAAAACTGCAAGCTGAAAAAGAAACTGCCCTCGAATTCCCGGCCT GCTCACAACTCTGCTGACTTGGATCCATTTAAATTCCGCTGGTTGATTCCCATATCTGCGCTTCAAGTTA GACTGGGGAACACGGCAGGGACTGAAAATAATTCCACGTGGGAGCTGATTCATACCAAGTCGGAAATTGA AGGACGGCCAGAAACCATCTTTCAACTGTGCTGCAGTGACAGCGAGAACAAAACCAGCATTGTTAAGGTG ATTCGTTCTATTCTGAGAGAGAACTTCCGGCGCCACATAAAGTGTGAGCTGCCACTGGAGAAGACGTGTA AGGACCGGCTAGTACCTCTTAAGAACCGAGTTCCTGTTTCAGCCAAATTAGCCTCGTCCAGGTCGTTGAA GGGCCTCAGAACATCCTCCAGCAGCGAGTGGCCCAGCGAGCCCAGCAAGGGCAACTCACTGGACTCAGAT GAGTGCAGCCTGAGCAGTGGCACCCAGAGTAGCGGCTGCCCCGTAGCCGAGAGCAGGCGAGACTCTAAGA GCACCGAGCTGGAGAAAGACGCTCAGGAGGGCCTGGCGGAGTTTCCAGATGGTCTTATCAAAGAAAGCGA CATTCTGAGTGATGAAGATGAGGACTTCCACCACCCTCTGAAACAGGGTAGCCCTACTAAGGACATTGAG ATTCAGTTCCAGAGACTGAAAATCTCTGAGGAATCCGACGTGCACCCAGTTGGGCAGCAGCCTCTCACAG AGTCAGGTGAACAGCCCAAGCTGGTCAGGGGCCATTTTTGCCCCATTAAACGGAAAGCAAACAGCACCAA GAGGGGCAGAGGAACTTTGCTCAAGGCGCAGACTCGTCACCAGTCCCTGGACAGCCACCCAGAAACTGCC AGCATTGATCTAAACTTGGTCCTGGAGAGAGAATTCAGTGTCCAGAGCTTAACTTCAGTCGTCAATGAGG AGGGTTTTTATGAAACACAGAGCCATGGCAAATCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212067 representing NM_011878
Red=Cloning site Green=Tags(s) MGNSESQYTFQGSKNHSNTVTGAKQKPCSLKIRSVHAKDEKSLHGWTHGSSGAGYKSRSLARSCLSHFKN HQPYATRLSGPTCKVSKGTTYSKHRANTPGNDFQGNSGAFLPENGFHYVDRESEESHITSNGHLLTCYGR KESLASTPPGEDHRSPRVLIKTLGKLDGCLRVEFHNGGNPHKGTSEDPSGPVRLLRYSPTLASETCPVRE TRRHSAAGSPSSQRPSPTDSRLRSSKGSSLSSESSWYDSPWGNAGEVSEVEGSFLAPSTPDPSLPSSFPP SDTKKPFNQSSSLSSLRELYKDPNLGCRSPSGTCLSSNEYISSQVSLNNRVSFASDMDVPSRVDHRDPLH YSSFTLPCRKSKALTEDAAKKDTLKARMRRFSDWTGSLSRKKRKLQEPRSMEGSEYFDSHSDGLNAEGQV PAQTSSLLWSGGSAQTLPHRSESTHAISVDPLRQNIYENFMRELEMSRSNTEHVETSTETMESSSESVSS LEQLDLLFEKEQGVVRKAGWLFFKPLVTLQKERKLELVARRKWKQYWVTLKGCTLLFYETYGKNSTEQNS APRCALFAEDSIVQSVPEHPKKEHVFCLSNSCGDVYLFQATSQTDLENWVTAIHSACASLFAKKHGKEDT VRLLKSQTRSLLQKIDMDSKMKKMAELQLSVVSDPKNRKAIENQIRQWEQNLEKFHMDLFRMRCYLASLQ GGELPNPKSLLAATSRPSKLALGRLGVLSVSSFHALVCSRDDSTLRKRTLSLTQRGKSKKGIFSSLKGLD TLARKGREKRASITQMFDSSHSHGFLGTQLPQKSTNSNKAHDLHLYGSAVDSALRDSMWEVQTYVHFQDN EGVTVTIKPEHRVEDVLALVCKMRQLEPTHYGLQLRKVVDKSVEWCVPALYEYMQEQVYDEIEVFPLSVY DVQLTKTGDMTDFGFAVTVQVDEHQHLNRIFISDVLPDSLAYGGGLRKGNEITSLNGEPVSDLDIQQMEA LFSEKSVGLTLVARPVTTRRTLCASWSDSDLFSRDQKSLPPSPNQSQLLEEFLDNFRKTATSDFSNVPEI TTGLKRSQTEGTLDQVPHREKMEQTFLSADQIAELCRDLNNTHTNSMEAPTESHDPPPRPLARHLSDADR LRKVIQELVDTEKSYVKDLSCLFELYLEPLQNETFLTQDEMESLFGSLPEMLEFQKVFLETLEDAISASS DFSVLETPSQFRKLLFSLGGSFLYYADHFKLYSGFCANHIKVQRVLERAKTDKAFKAFLDARNPTKQHSS TLESYLIKPVQRVLKYPLLLKELVSLTDHESEEHYHLTEALKAMEKVASHINEMQKIYEDYGMVFDQLVA EQSGTEKEVTELSMGELLMHSTVSWLNPFLSLGKARKDIELTVFVFKRAVILVYKENCKLKKKLPSNSRP AHNSADLDPFKFRWLIPISALQVRLGNTAGTENNSTWELIHTKSEIEGRPETIFQLCCSDSENKTSIVKV IRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLASSRSLKGLRTSSSSEWPSEPSKGNSLDSD ECSLSSGTQSSGCPVAESRRDSKSTELEKDAQEGLAEFPDGLIKESDILSDEDEDFHHPLKQGSPTKDIE IQFQRLKISEESDVHPVGQQPLTESGEQPKLVRGHFCPIKRKANSTKRGRGTLLKAQTRHQSLDSHPETA SIDLNLVLEREFSVQSLTSVVNEEGFYETQSHGKS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011878 |
| ORF Size | 5145 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011878.2, NP_036008.2 |
| RefSeq Size | 6128 bp |
| RefSeq ORF | 5148 bp |
| Locus ID | 24001 |
| UniProt ID | Q6ZPF3 |
| Gene Summary | Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates specifically RAC1, but not CDC42 and RHOA. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane.[UniProtKB/Swiss-Prot Function] |
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