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Tlk2 (NM_011903) Mouse Tagged ORF Clone
CAT＃: MG215868
- TrueORF®
Tlk2 (tGFP-tagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2) transcript variant B, (10ug)
ORF Plasmid: DDK
"NM_011903" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4933403M19Rik; PKUalpha; Tlk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215868 representing NM_011903
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAAGAACTGCATAGCCTGGACCCACGAAGGCAGGAATTACTAGAGGCCAGGTTCACTGGAGTTG GTGTAAGTAAGGGGCCACTCAACAGTGAGTCTTCCAACCAGAGTCTGTGCAGCGTGGGGTCGTTGAGTGA TAAAGAAGTAGAGACTCCTGAGAAAAAGCAGAATGACCAGCGAAATCGGAAAAGGAAAGCCGAGCCATAT GACACTAGCCAAGGGAAAGGCACTCCTAGGGGACATAAAATTAGTGATTACTTTGAGCGTCGAGCAGAAC AGCCTCTGTATGGTTTAGATGGCAGTGCAGCAAAGGAGGCCTCAGAAGAGCAGTCTGCTCTGCCCACCCT CATGTCAGTGATGTTAGCAAAACCGCGACTTGACACAGAGCAGTTAGCGCCACGGGGAGCTGGCCTCTGC TTCACTTTCGTCTCTGCTCAACAAAACAGTCCTTCTTCTACGGGTTCTGGCAATACAGAACATTCTTGCA GCTCCCAAAAACAGATCTCCATCCAGCACAGACAGACCCAGTCTGACCTCACAATAGAAAAAATATCTGC ACTAGAAAACAGTAAGAACTCTGACTTAGAGAAGAAGGAAGGAAGAATAGATGATTTATTAAGAGCCAAC TGTGATTTGAGACGACAGATAGATGAACAGCAAAAGATGCTAGAGAAATACAAGGAACGATTAAATAGAT GTGTCACCATGAGCAAGAAGCTCCTTATAGAAAAGTCAAAACAAGAGAAGATGGCGTGCAGAGATAAGAG CATGCAGGACCGATTGCGATTAGGCCACTTTACTACTGTCCGGCATGGAGCCTCGTTTACTGAGCAGTGG ACAGATGGTTATGCTTTCCAAAACCTCATCAAGCAACAGGAAAGGATAAATTCACAGAGAGAAGAGATAG AAAGGCAACGGAAAATGTTAGCAAAACGGAAACCTCCTGCCATGGGTCAGGCCCCTCCTGCAACCAATGA GCAGAAACAACGGAAAAGCAAGACTAATGGAGCTGAAAATGAAACGTTAACGTTAGCCGAGTACCATGAA CAAGAGGAAATCTTCAAACTTAGATTAGGTCATCTTAAGAAAGAGGAAGCAGAAATCCAGGCAGAGCTGG AAAGGCTGGAAAGGGTTAGGAATCTACACATCAGGGAATTAAAAAGGATACATAATGAAGACAATTCGCA GTTTAAAGACCATCCAACACTAAATGACAGATATTTGTTGTTACATCTTTTGGGTAGAGGAGGTTTCAGT GAAGTTTACAAGGCATTTGATCTAACGGAGCAAAGATATGTAGCTGTGAAAATTCACCAGTTAAATAAAA ACTGGAGAGATGAGAAAAAGGAGAATTACCACAAGCATGCGTGTAGGGAATACCGGATTCACAAGGAGCT GGACCACCCCAGGATAGTGAAGCTGTATGATTACTTTTCACTGGACACTGACTCGTTTTGTACAGTATTA GAATACTGTGAAGGGAATGACCTGGACTTCTACCTAAAACAGCACAAATTAATGTCGGAGAAAGAAGCCC GATCCATTATTATGCAGATTGTGAATGCTTTAAAGTACTTAAATGAAATAAAACCTCCCATCATACACTA TGACCTCAAACCAGGTAATATCCTTTTAGTAAATGGTACAGCATGTGGAGAGATAAAAATTACAGATTTT GGTCTTTCCAAGATCATGGATGATGATAGCTACAATTCAGTGGATGGCATGGAGCTGACGTCACAAGGAG CTGGTACTTATTGGTATTTACCACCAGAGTGTTTTGTGGTTGGGAAAGAGCCACCAAAGATCTCAAATAA AGTCGATGTTTGGTCAGTGGGTGTGATCTTCTACCAGTGTCTTTATGGGAGGAAGCCTTTTGGCCATAAC CAGTCCCAGCAAGATATTCTACAAGAGAATACTATTCTTAAGGCTACTGAAGTACAGTTCCCGCCAAAGC CAGTAGTAACACCTGAAGCAAAGGCATTTATCAGGAGATGTCTGGCCTATCGAAAGGAAGATCGCATTGA TGTGCAGCAGCTGGCCTGTGACCCCTACTTGTTGCCTCACATCCGAAAGTCAGTCTCCACAAGTAGCCCT GCAGGAGCTGCTATTGCATCAACCTCTGGGGCATCCAATAACAGTTCTTCGAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215868 representing NM_011903
Red=Cloning site Green=Tags(s) MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPY DTSQGKGTPRGHKISDYFERRAEQPLYGLDGSAAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLC FTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRAN CDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQW TDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHE QEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFS EVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVL EYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDF GLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHN QSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSP AGAAIASTSGASNNSSSN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011903 |
| ORF Size | 2154 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011903.3, NP_036033.2 |
| RefSeq Size | 5379 bp |
| RefSeq ORF | 2157 bp |
| Locus ID | 24086 |
| Gene Summary | Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (By similarity). Negative regulator of amino acid starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221003 | Tlk2 (untagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant B, (10ug) |
CNY 9,220.00 |
|
| MR215868 | Tlk2 (Myc-DDK-tagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant B |
CNY 7,130.00 |
|
| MR215868L3 | Lenti ORF clone of Tlk2 (Myc-DDK-tagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant B |
CNY 9,030.00 |
|
| MR215868L4 | Lenti ORF clone of Tlk2 (mGFP-tagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant B |
CNY 9,030.00 |
