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Pla2g4f (NM_001024145) Mouse Tagged ORF Clone
CAT＃: MG220245
- TrueORF®
Pla2g4f (tGFP-tagged) - Mouse phospholipase A2 group IVF (Pla2g4f), (10ug)
ORF Plasmid: DDK
"NM_001024145" in other vectors (4)
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CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4732472I07Rik; Gm647; Pla2zeta |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220245 representing NM_001024145
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGACTCTCCAGCCAAAGTGGCTGGCAGGCAAGGGACTTCCCCTTCTTGGAGCCATACTGCTAC GGAAGACAGAAAAGAGCGAACCACAATGGAAGCATAGGCGGGAAACCCACCCGTACTACGACCTTCAAGT GAAGGTGCTGAGGGCCAGAAACATCCAGCACACAGATAAGTTGTCCAAAGCCGACTGCTATGTTCGACTG TGGCTGCCCACGGCTTCTGTTAGCCCCAGTCAGACAAGGACAGTGGTTAACAGCAGTGATCCAGAATGGA ATGAGACCTTTCACTATCAGATCCACGGCGCTGTGAAGAACGTCTTGGAGCTTGCCCTTTATGACGAGGA TGTCCTGGACAGTGACAATGTCTTCTCCATTCTGTTTGACATGAGTACTCTCCAGCTAGGCCAGCCTTGC ACAAAAAACTTCACCAGGCAGCAGGATCCAAAGGAGCTGGAAGTAGAATTTACTCTGGAAAAGAGTCAGA CGCCTGCATCTGAAGTTGTCACCAATGGTGTCCTGGTGGCTCACCCCTGTCTGAGAATTCAGGGCACAGT CACTGGAGACAAGACAGCCTCCCTTGGAGAGTTGGGCTCCAGGCAGATCCAGCTGGCAGTGCCTGGGGCC TATGAAAAGCCACAGCCTCTGCAGCCGACCTCGGAGCCAGGCCTCCCAGTGAACTTTACCTTCCACATGA ACCCAGTGCTGAGCCCCAAGCTGCACATAAAGCTGCAAGAACAGCTCCAAGTCTTCCATAGTGGCCCGAG TGATGAGCTGGAAGCTCAGACCAGCAAGATGGACAAGGCAAGCATCCTGCTCTCCTCTCTGCCCCTCAAC GAGGAGTTAACGAAACTTGTGGACCTGGAGGAGGGCCAGCAGGTGACTCTTAGGATGAAGGCAGACATGA GCAGCTCTGGGGACTTGGACCTGCGCCTTGGTTTTGACCTCTGTGATGGGGAGCAGGAATTTCTGGACAA GAGGAAGCAGGTGGCGTCCAAGGCCCTGCAGCGGGTGATGGGATTGAGTGAGGCTCTGCACTGTGACCAG GTACCCGTGGTAGCCGTGTTAGGCTCTGGGGGTGGAACCAGAGCCATGACTTCCCTGTACGGCAGCCTGG CTGGGCTGCAGGAGCTTGGTCTTCTGGATGCCGTGACCTACCTGAGTGGGGTATCTGGGTCTTCCTGGTG CATCTCTACACTCTACAGGGATCCATCCTGGTCCCAGAAGGCTTTGCAGGGCCCCATTAAATATGCCTCA GAGCGAGTCTGCAGCAGTAAAATTGGGATGCTGTCCCCAAAGCAGTTTGAATACTACTCCCGGGAAAAGA GAGCCTGGGAGAGCAGGGGACACAGCATGTCCTTCACTGACTTGTGGGGCCTCATCATTGAGTATTTCCT GAACCAGGAGGAAAACCCTGCCAAGCTGTCAGACCAGCAAGAAACGGTCAGCCAGGGTCAGAACCCATAC CCCATCTATGCCAGCATTAATGTCCACAAAAACATCAGTGGGGACTACTTTGCAGAGTGGTGTGAGTTCA CCCCCTATGAGGTCGGTTTCCCCAAGTACGGGGTTTACGTTCCCACGGAACTCTTTGGCTCTGAATTCTT CATGGGCCGGCTGCTGCATTTCTGGCCAGAGCCCCGCATCTGTTACCTGCAGGGTATGTGGGGAAGTGCT TTTGCAGCCAGCCTGTATGAGATCTTCCTGAAGCTGGGAGGCCTAAGCCTGAGCTTTCTGGACTGGCACA GGGGGAGTGTCAGTGTCACAGATGACTGGCCAAAGTTACGGAAGCAGGACCCCACACGGCTGCCTACCAG GCTCTTCACGCCAATGAGTTCCTTCTCTCAGGCTGTGCTGGACATATTCACCTCCCGTATTACTTGTGCC CAGACCTTTAACTTTACCCGAGGTCTCTGCATGTACAAAGACTACACAGCTAGAAAGGACTTCGTGGTCT CTGAAGATGCATGGCATTCACATAACTATGGATACCCTGATGCCTGTCCCAACCAGCTCACACCCATGAA GGACTTCCTGTCCCTAGTAGATGGAGGCTTTGCTATCAACTCGCCATTTCCACTGGTCCTGCAGCCGCAG CGGGCTGTGGACCTCATTGTGTCCTTTGACTATTCCTTGGAAGGTCCTTTTGAGGTCCTGCAGGTGACAG AGAAGTACTGCCGGGACCGAGGGATCCCCTTCCCAAGGATTGAGGTGGACCCCAAGGACTCTGAAGACCC CCGTGAATGCTATCTGTTTACCGAGGCAGAGGACCCCTGCTCGCCCATCGTGCTGCATTTCCCTCTGGTC AACAGGACCTTTCGCACGCACCTGGCCCCAGGTGTGGAACGACAAACAGCTGAGGAGAAGGCCTTCGGGG ACTTTATCATCAACGGGCCAGATACTGCCTATGGCATGATGGATTTCACCTATGAGCCCAAGGAATTTGA TCGGCTGGTGACCCTGAGCCGATACAACGTCTTGAACAACAAGGAGACTATCAGGCATGCCCTCCAGCTG GCTCTGGACCGGCGGCGGCAGGCTGGGGGAAGGGTTGGGGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220245 representing NM_001024145
Red=Cloning site Green=Tags(s) MPWTLQPKWLAGKGLPLLGAILLRKTEKSEPQWKHRRETHPYYDLQVKVLRARNIQHTDKLSKADCYVRL WLPTASVSPSQTRTVVNSSDPEWNETFHYQIHGAVKNVLELALYDEDVLDSDNVFSILFDMSTLQLGQPC TKNFTRQQDPKELEVEFTLEKSQTPASEVVTNGVLVAHPCLRIQGTVTGDKTASLGELGSRQIQLAVPGA YEKPQPLQPTSEPGLPVNFTFHMNPVLSPKLHIKLQEQLQVFHSGPSDELEAQTSKMDKASILLSSLPLN EELTKLVDLEEGQQVTLRMKADMSSSGDLDLRLGFDLCDGEQEFLDKRKQVASKALQRVMGLSEALHCDQ VPVVAVLGSGGGTRAMTSLYGSLAGLQELGLLDAVTYLSGVSGSSWCISTLYRDPSWSQKALQGPIKYAS ERVCSSKIGMLSPKQFEYYSREKRAWESRGHSMSFTDLWGLIIEYFLNQEENPAKLSDQQETVSQGQNPY PIYASINVHKNISGDYFAEWCEFTPYEVGFPKYGVYVPTELFGSEFFMGRLLHFWPEPRICYLQGMWGSA FAASLYEIFLKLGGLSLSFLDWHRGSVSVTDDWPKLRKQDPTRLPTRLFTPMSSFSQAVLDIFTSRITCA QTFNFTRGLCMYKDYTARKDFVVSEDAWHSHNYGYPDACPNQLTPMKDFLSLVDGGFAINSPFPLVLQPQ RAVDLIVSFDYSLEGPFEVLQVTEKYCRDRGIPFPRIEVDPKDSEDPRECYLFTEAEDPCSPIVLHFPLV NRTFRTHLAPGVERQTAEEKAFGDFIINGPDTAYGMMDFTYEPKEFDRLVTLSRYNVLNNKETIRHALQL ALDRRRQAGGRVGG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001024145 |
| ORF Size | 2565 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001024145.2, NP_001019316.1 |
| RefSeq Size | 3125 bp |
| RefSeq ORF | 2568 bp |
| Locus ID | 271844 |
| UniProt ID | Q50L41 |
| Gene Summary | Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222204 | Pla2g4f (untagged) - Mouse phospholipase A2, group IVF (Pla2g4f), (10ug) |
CNY 10,930.00 |
|
| MR220245 | Pla2g4f (Myc-DDK-tagged) - Mouse phospholipase A2, group IVF (Pla2g4f) |
CNY 6,264.00 |
|
| MR220245L3 | Lenti ORF clone of Pla2g4f (Myc-DDK-tagged) - Mouse phospholipase A2, group IVF (Pla2g4f) |
CNY 9,410.00 |
|
| MR220245L4 | Lenti ORF clone of Pla2g4f (mGFP-tagged) - Mouse phospholipase A2, group IVF (Pla2g4f) |
CNY 9,410.00 |
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