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Dcp2 (NM_027490) Mouse Tagged ORF Clone
CAT＃: MG221051
- TrueORF®
Dcp2 (tGFP-tagged) - Mouse DCP2 decapping enzyme homolog (S. cerevisiae) (Dcp2), (10ug)
ORF Plasmid: DDK
"NM_027490" in other vectors (4)
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CNY 4,560.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2410015D23Rik; 5730537H01Rik; AL118268 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221051 representing NM_027490
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCAAACGCCTGGAGATTCCCGGCAGCGTCCTGGATGATCTCTGCAGCCGATTTATTTTGCATA TTCCCAGTGAAGAAAGAGACAATGCGATCCGAGTGTGCTTCCAGATTGAACTTGCCCATTGGTTTTACTT GGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGCGGGATAAGAGACTTTGCCAAAGCTGTTTTC AGTCACTGTCCATTTCTGCTGCCACAAGGTGAAGATGTGGAAAAGATTTTGGATGAATGGAAGGAATATA AAATGGGAGTCCCGACTTACGGTGCAATTATTCTCGATGAGACACTGGAGAATGTACTGCTGGTTCAGGG GTACCTGGCAAAGTCAGGCTGGGGATTCCCAAAAGGAAAAGTAAATAAAGAAGAGGCGCCTCATGACTGT GCGGCGAGAGAGGTCTTCGAGGAAACTGGTTTTGATATAAAAGACTATATTTGTAAGGATGATTACATTG AACTGCGAATCAATGACCAGCTTGCTCGCTTGTACATCATTCCAGGAGTCCCAAAAGACACAAAATTTAA CCCCAAAACCAGAAGAGAAATTAGGAATATTGAGTGGTTCTCCATTGAGAAATTGCCCTGTCATAGAAAT GACATGACTCCCAAGTCCAAGCTTGGCTTGGCACCTAATAAGTTTTTTATGGCCATTCCCTTTATCAGAC CACTAAGGGACTGGCTGTCTCGAAGATTTGGAGATTCCTCGGACAGTGACAATGGGTTTTCCTCAGCTGG TAGCACACCAGCTAGACCCACCGTGGAGAAATTGAGTCGAACAAAATTCCGCCACAGCCAGCAGCTGTTT CCTGAAGGTTCCCCGAGTGACCAGTGGGTTAAGCACCGGCAGCCACTGCAGCAGAAGTCACACAGTAACC ACGGGGAGGTGTCGGACCTTCTCAAAGCAAAGAATCAAAATATGAGAGGGAATGGCAGAAAACAGTATCA AGACTCACCTAATCAAAAGAAGAGAGCGAACGGAGTCCATGGTCAGCCGGCAAAGCAGCAGAATCCCTTG GTGAAATGTGAAAAGAAGCTGCATCCACGGAAACTTCAGGACAACTTCGAGACAGATGCCACATGTGACC TGCCTTGTTCTGGTGAAGAGCCGTCGGTAGAACATGCTGAGGGACATTCTGTGGCATGCAACGGACATTG CAAGTTCCCATTTTCATCCAGAGCCTTTCTGAGTTTCAAGTTTGACCAAAATGCTATAATGAAAATCTTG GACCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221051 representing NM_027490
Red=Cloning site Green=Tags(s) MEPKRLEIPGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKAVF SHCPFLLPQGEDVEKILDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKEEAPHDC AAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGVPKDTKFNPKTRREIRNIEWFSIEKLPCHRN DMTPKSKLGLAPNKFFMAIPFIRPLRDWLSRRFGDSSDSDNGFSSAGSTPARPTVEKLSRTKFRHSQQLF PEGSPSDQWVKHRQPLQQKSHSNHGEVSDLLKAKNQNMRGNGRKQYQDSPNQKKRANGVHGQPAKQQNPL VKCEKKLHPRKLQDNFETDATCDLPCSGEEPSVEHAEGHSVACNGHCKFPFSSRAFLSFKFDQNAIMKIL DL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027490 |
| ORF Size | 1266 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027490.1, NP_081766.1 |
| RefSeq Size | 8643 bp |
| RefSeq ORF | 1269 bp |
| Locus ID | 70640 |
| UniProt ID | Q9CYC6 |
| Gene Summary | Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:21070968). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:21070968). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (By similarity). Plays a role in replication-dependent histone mRNA degradation (By similarity). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed:21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211089 | Dcp2 (untagged) - Mouse DCP2 decapping enzyme homolog (S. cerevisiae) (Dcp2), (10ug) |
CNY 3,990.00 |
|
| MR221051 | Dcp2 (Myc-DDK-tagged) - Mouse DCP2 decapping enzyme homolog (S. cerevisiae) (Dcp2) |
CNY 5,488.00 |
|
| MR221051L3 | Lenti ORF clone of Dcp2 (Myc-DDK-tagged) - Mouse DCP2 decapping enzyme homolog (S. cerevisiae) (Dcp2) |
CNY 6,080.00 |
|
| MR221051L4 | Lenti ORF clone of Dcp2 (mGFP-tagged) - Mouse DCP2 decapping enzyme homolog (S. cerevisiae) (Dcp2) |
CNY 6,080.00 |
