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Gne (NM_001190414) Mouse Tagged ORF Clone
CAT＃: MG221799
- TrueORF®
Gne (tGFP-tagged) - Mouse glucosamine (Gne) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001190414" in other vectors (3)
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CNY 7,980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221799 representing NM_001190414
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGAACGGGAACAACCGAAAGCTCCGGGTTTGCGTTGCCACCTGCAACCGAGCTGACTACTCCA AACTGGCCCCGATCATGTTCGGCATCAAGACAGAGCCCGCGTTCTTTGAGTTGGACGTGGTGGTGCTCGG CTCCCACCTGATTGACGACTATGGAAACACATACCGCATGATTGAGCAAGATGACTTTGACATTAACACC AGGCTCCACACGATTGTTAGAGGGGAAGATGAAGCGGCCATGGTAGAGTCGGTAGGCCTAGCGCTCGTGA AGCTACCGGACGTCCTCAATCGCCTGAAGCCCGACATCATGATTGTTCACGGAGACCGATTTGACGCCCT TGCTCTGGCTACGTCTGCTGCCTTGATGAACATCCGCATCCTTCACATTGAAGGAGGCGAGGTCAGCGGG ACCATTGATGACTCTATCAGACACGCCATAACAAAACTGGCTCACTACCATGTGTGCTGCACTAGAAGTG CAGAGCAGCACCTGATCTCTATGTGCGAGGACCACGACCGCATCCTGTTGGCAGGCTGCCCTTCCTATGA CAAACTGCTCTCCGCCAAGAACAAAGACTATATGAGCATCATTCGGATGTGGCTAGGCGATGATGTAAAA TGTAAGGATTACATCGTTGCCCTGCAGCATCCCGTGACCACTGACATTAAGCATTCCATAAAGATGTTTG AGCTAACACTGGATGCCCTGATCTCGTTTAACAAGAGGACCCTAGTTCTGTTTCCAAATATCGATGCAGG CAGCAAGGAGATGGTTCGAGTGATGCGGAAGAAGGGCATCGAGCATCACCCCAATTTCCGTGCAGTCAAG CACGTCCCGTTTGACCAGTTCATACAGCTGGTCGCCCACGCTGGCTGCATGATTGGGAATAGCAGCTGCG GCGTGCGAGAGGTTGGCGCTTTCGGAACACCCGTGATCAACCTGGGCACAAGGCAGATAGGAAGAGAAAC CGGGGAGAATGTTCTTCATGTCAGGGATGCTGACACCCAAGATAAAATATTGCAAGCACTACACCTCCAG TTCGGCAAACAGTACCCTTGCTCAAAGATATATGGGGATGGGAATGCTGTTCCAAGGATTTTAAAGTTTC TCAAATCCATTGACCTTCAAGAGCCACTACAGAAGAAATTCTGCTTCCCCCCTGTAAAGGAGAACATCTC TCAAGACATTGACCACATCCTGGAAACTCTGAGTGCCTTGGCTGTTGATCTTGGCGGGACAAACCTGAGG GTGGCAATAGTTAGCATGAAGGGTGAAATCGTTAAGAAGTACACTCAGTTCAACCCTAAAACCTATGAAG AAAGGATTAGTTTAATCCTGCAGATGTGTGTGGAAGCTGCCGCGGAAGCTGTGAAACTCAATTGCAGAAT TCTGGGAGTAGGCATCTCCACAGGTGGCCGCGTGAATCCCCAGGAAGGAGTTGTGCTGCATTCAACCAAG CTGATCCAGGAATGGAACTCCGTGGACCTCAGGACACCCCTCTCCGACACCCTGCATCTCCCCGTGTGGG TGGACAATGACGGCAACTGTGCCGCCATGGCAGAGAGGAAGTTCGGCCAAGGAAAAGGACAGGAGAACTT CGTGACGCTCATCACGGGGACAGGGATCGGTGGGGGGATCATCCACCAGCACGAACTGATCCACGGCAGC TCCTTCTGCGCGGCGGAGCTCGGCCATCTCGTGGTGTCCCTGGACGGTCCTGACTGCTCCTGTGGAAGCC ATGGGTGCATCGAAGCGTACGCCTCTGGAATGGCCTTGCAGAGGGAAGCAAAGAAACTCCATGATGAGGA CCTGCTCTTGGTGGAAGGGATGTCAGTACCAAAAGACGAAGCTGTGGGTGCCCTCCATCTCATCCAGGCT GCCAAGCTGGGCAACGTGAAGGCCCAGAGCATCTTACGAACAGCTGGAACTGCTTTGGGACTTGGGGTTG TGAACATCCTCCACACTATGAATCCTTCCCTGGTGATCCTGTCTGGAGTCCTGGCCAGTCACTACATCCA CATCGTGAAGGACGTCATCCGCCAGCAAGCCTTGTCCTCCGTGCAGGATGTGGACGTGGTGGTCTCAGAC TTGGTGGACCCGGCCCTGCTTGGCGCAGCCAGCATGGTTCTGGACTACACAACGCGCAGGATCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221799 representing NM_001190414
Red=Cloning site Green=Tags(s) MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINT RLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSG TIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVK CKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVK HVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQ FGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLR VAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTK LIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGS SFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQA AKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSD LVDPALLGAASMVLDYTTRRIH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190414 |
| ORF Size | 2166 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190414.2 |
| RefSeq Size | 2818 bp |
| RefSeq ORF | 2169 bp |
| Locus ID | 50798 |
| UniProt ID | Q91WG8 |
| Gene Summary | Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Required for normal sialylation in hematopoietic cells (By similarity). Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. Plays an essential role in early development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR221799 | Gne (Myc-DDK-tagged) - Mouse glucosamine (Gne), transcript variant 1 |
CNY 5,288.00 |
|
| MR221799L3 | Lenti ORF clone of Gne (Myc-DDK-tagged) - Mouse glucosamine (Gne), transcript variant 1 |
CNY 9,120.00 |
|
| MR221799L4 | Lenti ORF clone of Gne (mGFP-tagged) - Mouse glucosamine (Gne), transcript variant 1 |
CNY 9,120.00 |
