United States
Germany
Japan
United Kingdom
China
Hap1 (NM_010404) Mouse Tagged ORF Clone
CAT＃: MG222609
- TrueORF®
Hap1 (tGFP-tagged) - Mouse huntingtin-associated protein 1 (Hap1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_010404" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,800.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HAP; HAP-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222609 representing NM_010404
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCCGAAAGAGCAGGTGCAGAGCGGTGCGGGAGACGGGACAGGGTCGGGGGACCCAGCAGCAGGCA CCCCCACGACCCAGCCTGCAGTTGGTCCCGCTCCGGAGCCCTCGGCGGAGCCCAAACCTGCTCCAGCGCA GGGAACCGGGTCCGGACAAAAATCAGGATCCCGAACCAAGACAGGAAGCTTTTGTCGGTCCATGATCATT GGTGATTCGGACGCACCATGGACCCGCTACGTATTCCAGGGGCCTTACGGTCCCCGGGCCACTGGCCTGG GCACTGGAAAGGCCGAGGGAATCTGGAAGACACCAGCCGCGTACATCGGCCGGAGGCCCGGCGTGTCCGG CCCTGAGCGTGCGGCGTTTATTCGAGAGCTGCAGGAAGCGTTGTGTCCTAATCCACCACCCACGAAGAAG ATCACCGAAGATGATGTCAAAGTGATGTTGTATTTGCTGGAAGAGAAAGAACGGGACCTGAACACAGCCG CCCGGATCGGCCAGTCCCTGGTGAAACAGAACAGTGTCTTGATGGAGGAGAATAATAAGCTGGAAACCAT GCTGGGCTCAGCCAGGGAGGAGATTTTACATCTCCGGAAGCAGGTGAACCTGCGAGATGACCTTCTTCAG CTCTACTCAGACTCTGATGACGATGATGATGAGGAAGACGAGGAAGACGAGGAAGAGGGCGAAGAGGAGG AACGAGAAGGACAGAGGGATCAAGACCAGCAGCACGACCACCCCTATGGTGCCCCCAAGCCACACCCTAA GGCTGAGACAGCGCACCGCTGCCCACAGCTGGAAACCCTGCAGCAGAAGCTCAGGCTTCTGGAGGAAGAG AACGACCACCTGCGAGAGGAGGCCTCCCACCTTGACAACCTGGAGGACGAAGAGCAGATGCTCATTCTGG AATGTGTGGAGCAGTTCTCTGAAGCCAGCCAGCAGATGGCAGAGCTATCGGAAGTGCTGGTGTTGAGGCT GGAAGGCTATGAGAGGCAGCAGAAAGAGATCACTCAGCTGCAGGCCGAGATCACCAAGCTACAACAGCGT TGTCAGTCTTATGGGGCCCAGACAGAGAAACTGCAGCAGATGCTGGCCTCAGAGAAGGGGATCCACTCGG AGAGCCTGCGAGCTGGCTCCTACATGCAGGATTATGGGAGCAGGCCTCGTGACCGCCAGGAGGATGGGAA GAGTCATCGCCAGCGCTCCTCCATGCCCGCAGGCTCTGTCACCCACTATGGATACAGTGTGCCTCTGGAT GCACTTCCAAGTTTCCCAGAGACACTGGCTGAGGAGCTCCGAACATCTCTGAGGAAGTTCATCACTGACC CTGCGTATTTCATGGAGAGACGTGACACTCACTGCAGGGAGGGGCGGAAGAAGGAGCAGAGGGCGATGCC ACCCCCACCGGCTCAAGATCTCAAGCCACCTGAAGATTTTGAGGCTCCAGAGGAGTTGGTTCCTGAGGAG GAGCTGGGGGCCATCGAAGAGGTGGGGACAGCTGAGGATGGGCAGGCAGAAGAGAACGAGCAGGCGTCTG AGGAGACCGAGGCCTGGGAGGAGGTGGAACCGGAGGTGGACGAGACCACAAGGATGAATGTGGTGGTCTC CGCCCTGGAGGCCAGTGGCCTGGGCCCTTCCCACCTGGACATGAAGTATGTCCTCCAGCAACTGTCCAAC TGGCAGGACGCCCACTCTAAGCGGCAGCAGAAGCAGAAGGTGGTCCCAAAAGGTGAGTGTTCCCGCAGAG GACACCCTCCTGCCAGTGGGACAAGCTTTCGATCATCAACCATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222609 representing NM_010404
Red=Cloning site Green=Tags(s) MRPKEQVQSGAGDGTGSGDPAAGTPTTQPAVGPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGSFCRSMII GDSDAPWTRYVFQGPYGPRATGLGTGKAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPPPTKK ITEDDVKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQ LYSDSDDDDDEEDEEDEEEGEEEEREGQRDQDQQHDHPYGAPKPHPKAETAHRCPQLETLQQKLRLLEEE NDHLREEASHLDNLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQR CQSYGAQTEKLQQMLASEKGIHSESLRAGSYMQDYGSRPRDRQEDGKSHRQRSSMPAGSVTHYGYSVPLD ALPSFPETLAEELRTSLRKFITDPAYFMERRDTHCREGRKKEQRAMPPPPAQDLKPPEDFEAPEELVPEE ELGAIEEVGTAEDGQAEENEQASEETEAWEEVEPEVDETTRMNVVVSALEASGLGPSHLDMKYVLQQLSN WQDAHSKRQQKQKVVPKGECSRRGHPPASGTSFRSSTI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010404 |
| ORF Size | 1794 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010404.3, NP_034534.1 |
| RefSeq Size | 3774 bp |
| RefSeq ORF | 1797 bp |
| Locus ID | 15114 |
| UniProt ID | O35668 |
| Gene Summary | The protein encoded by this gene was first identified as a neuronal protein that binds the HD protein huntingtin. The protein also interacts with kinesin light chain, 14-3-3 proteins, and Abelson helper integration site 1 protein. The protein is involved in intracellular trafficking of vesicles and organelles, and lack of the protein results in neuronal death resembling the hypothalamic degeneration that occurs in Huntington's disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219500 | Hap1 (untagged) - Mouse huntingtin-associated protein 1 (Hap1), transcript variant 1, (10ug) |
CNY 7,700.00 |
|
| MR222609 | Hap1 (Myc-DDK-tagged) - Mouse huntingtin-associated protein 1 (Hap1), transcript variant 1 |
CNY 4,456.00 |
|
| MR222609L3 | Lenti ORF clone of Hap1 (Myc-DDK-tagged) - Mouse huntingtin-associated protein 1 (Hap1), transcript variant 1 |
CNY 7,130.00 |
|
| MR222609L4 | Lenti ORF clone of Hap1 (mGFP-tagged) - Mouse huntingtin-associated protein 1 (Hap1), transcript variant 1 |
CNY 7,130.00 |
