United States
Germany
Japan
United Kingdom
China
Tubb1 (NM_001080971) Mouse Tagged ORF Clone
CAT＃: MG224088
- TrueORF®
Tubb1 (tGFP-tagged) - Mouse tubulin beta 1 (Tubb1), (10ug)
ORF Plasmid: DDK
"NM_001080971" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810484G07Rik; M(beta)1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224088 representing NM_001080971
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGAGATCGTGCACATCCAGATTGGCCAGTGTGGCAACCAGATCGGAGCCAAGTTCTGGGAGGTGA TCGGGGAAGAACATGGGATTGACTGCGCTGGGAGCTACTGTGGGACGTCTGCTCTCCAGCTGGAGAGGAT CAGCGTTTACTACAACGAAGCCTACGGTAAGAAGTATGTGCCGCGAGCCGTCCTTGTGGACCTGGAACCC GGGACAATGGACAGCATCCGATCCAGCAGATTGGGAGTCCTCTTTCAGCCTGACAGTTTTGTTCATGGTA ACTCTGGTGCTGGTAACAACTGGGCGAAGGGCCACTACACAGAGGGAGCGGAGCTGATCGAAAATGTCAT GGATGTGGTAAGGAGGGAGAGCGAGAGCTGCGACTGCCTACAGGGTTTCCAGATCGTGCATTCTCTGGGT GGAGGCACGGGCTCAGGCATGGGCACCCTGCTCATGAACAAGATCAGGGAGGAGTACCCGGATCGGATCT TGAACTCCTTTAGCGTCATGCCATCACCCAAGGTATCAGACACGGTGGTAGAGCCCTACAATGCCGTGCT ATCCATCCACCAGCTGATAGAGAACACAGATGCCTGCTTCTGCATCGACAACGAAGCGCTCTATGACATT TGTTTCCGCACCCTGAGGCTGACGACACCCACCTACGGGGATCTCAACCACCTGGTGTCCTTGACCATGA GCGGAATCACGACTTCACTGCGCTTTCCCGGTCAGCTCAATGCAGACCTGCGCAAGCTGGCCGTGAATAT GGTGCCCTTTCCTCGTCTTCACTTCTTCATGCCTGGCTTCGCTCCACTCACAGCCCAGGGTAGCCAGCAG TACCGAGCCCTCTCAGTAGCGGAGCTCACCCAACAGATGTTCGATGCCCGCAACATCATGGCTGCCTGTG ACCCTCGCCGCGGCCGCTACCTGACTGTGGCTTGTATATTCCGGGGTAAGATGTCCACCAAGGAAGTGGA CCAACAGCTGCTGTCCATTCAGACAAGGAACAGCAACTGCTTTGTGGAATGGATTCCCAACAATGTCAAG GTAGCTGTGTGTGACATCCCGCCCCGTGGGCTGAACATGGCAGCTACCTTCCTGGGCAACAATACAGCCA TCCAAGAGCTCTTCACCAGGGTCTCTGAGCACTTCTCAGCTATGTTCAGAAGGAGAGCTTTTGTGCACTG GTACACCAGTGAAGGGATGGACATAAGTGAATTTGGGGAAGCTGAGAGTGATATCCATGACCTGGTGTCT GAGTACCAACAATTCCAAGATGTCAGAGCAGGGCTAGAGGACAGTGAAGAGGATGTGGAGGAGGCAGAGG TGGAGGCAGAAGACAAAGATCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224088 representing NM_001080971
Red=Cloning site Green=Tags(s) MREIVHIQIGQCGNQIGAKFWEVIGEEHGIDCAGSYCGTSALQLERISVYYNEAYGKKYVPRAVLVDLEP GTMDSIRSSRLGVLFQPDSFVHGNSGAGNNWAKGHYTEGAELIENVMDVVRRESESCDCLQGFQIVHSLG GGTGSGMGTLLMNKIREEYPDRILNSFSVMPSPKVSDTVVEPYNAVLSIHQLIENTDACFCIDNEALYDI CFRTLRLTTPTYGDLNHLVSLTMSGITTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTAQGSQQ YRALSVAELTQQMFDARNIMAACDPRRGRYLTVACIFRGKMSTKEVDQQLLSIQTRNSNCFVEWIPNNVK VAVCDIPPRGLNMAATFLGNNTAIQELFTRVSEHFSAMFRRRAFVHWYTSEGMDISEFGEAESDIHDLVS EYQQFQDVRAGLEDSEEDVEEAEVEAEDKDH TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080971 |
| ORF Size | 1353 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001080971.2, NP_001074440.1 |
| RefSeq Size | 1406 bp |
| RefSeq ORF | 1356 bp |
| Locus ID | 545486 |
| UniProt ID | A2AQ07 |
| Gene Summary | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216075 | Tubb1 (untagged) - Mouse tubulin, beta 1 (Tubb1), (10ug) |
CNY 5,800.00 |
|
| MR224088 | Tubb1 (Myc-DDK-tagged) - Mouse tubulin, beta 1 (Tubb1) |
CNY 5,488.00 |
|
| MR224088L3 | Lenti ORF clone of Tubb1 (Myc-DDK-tagged) - Mouse tubulin, beta 1 (Tubb1) |
CNY 6,370.00 |
|
| MR224088L4 | Lenti ORF clone of Tubb1 (mGFP-tagged) - Mouse tubulin, beta 1 (Tubb1) |
CNY 6,370.00 |
