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Srgap1 (NM_001081037) Mouse Tagged ORF Clone
CAT＃: MG224537
- TrueORF®
Srgap1 (tGFP-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 1 (Srgap1), (10ug)
ORF Plasmid: DDK
"NM_001081037" in other vectors (4)
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CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930572H05Rik; Arhgap13 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224537 representing NM_001081037
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACCCCGAGCAGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAA TCCGAGCCCAGCTGGTAGAACAACAAAAATGCCTGGAGCAGCAGACAGAGATGCGAGTTCAGCTGCTCCA GGACCTACAAGATTTCTTCCGGAAAAAAGCCGAAATTGAGACGGAATATTCTCGGAACCTGGAGAAGCTG GCAGAAAGGTTCATGGCCAAAACAAGAAGCACTAAGGACCATCAACAGTTCAAGAAAGACCAAAACCTGT TGTCTCCAGTGAACTGCTGGTATTTGCTCCTGAACCAAGTGAGAAGAGAGAGCAAAGACCACGCCACTTT GAGTGACATCTATCTGAACAATGTCATCATGCGGTTCATGCAGATAAGCGAGGACTCCACCCGGATGTTT AAAAAGAGCAAAGAGATTGCGTTCCAGCTTCACGAGGACCTCATGAAGGTTCTGAATGAGCTCTACACGG TGATGAAAACCTACCACATGTACCACTCAGAGAGCATCAGTGCAGAGAGTAAGCTGAAAGAAGCCGAGAA ACAAGAGGAGAAGCAGATTGGGAGGTCCGGCGACCCAGTCTTCCACATTAGGCTGGAAGAGAGGCATCAG AGGCGGAGCTCTGTGAAGAAAATTGAAAAAATGAAGGAAAAGAGACAAGCAAAATATTCAGAAAATAAGC TGAAATCGATCAAAGCCCGCAACGAGTATCTCCTAACACTTGAAGCAACCAACGCCTCCGTGTTCAAGTA TTACATCCATGATCTTTCAGACTTAATAGACTGCTGTGATCTCGGCTACCATGCAAGTCTGAACAGAGCC CTCAGAACGTACCTCTCGGCGGAGTACAACCTTGAAACCTCCAGACACGAAGGCTTAGACATCATTGAAA ACGCTGTGGACAATTTAGAGCCCAGGAGTGACAAGCAGAGGTTCATGGAGATGTACCCTGCGGCATTCTG CCCGCCAATGAAATTCGAGTTTCAGTCTCACATGGGTGATGAGGTGTGTCAGGTCAGCGCCCAGCAGCCC GTCCAGGCGGAGCTCATGCTCAGGAACCAACAGCTGCAGTCCCGACTGGCCACACTCAAGATCGAGAGCG AGGAGGTCAAGAAAACGACAGAAGCCACCCTCCAGACGATCCAAGATATGGTCACCATTGAGGACTATGA TGTGTCTGAGTGTTTCCAGCACAGCCGATCCACAGAGTCTGTGAAGTCCACTGTGTCAGAAACCTACCTG AGTAAACCCAGCATCGCCAAGAGACGAGCCAACCAGCAAGAAACGGAGCAGTTCTACTTCATGAAACTTC GAGAGTTCCTGGAAGGCAGTAACCTCATCACAAAGCTGCAGGCTAAGCATGACTTGCTGCAGAGGACTCT TGGAGAAGGCCATAGAGCTGAATATATGACTACAAGGCCTCCAAACGTCCCCCCTAAGCCCCAGAAACAC AGGAAGTCCAGGCCCCGCTCCCAGTATAATGCTAAGTTGTTTAATGGGGATTTGGAGACATTCGTCAAGG ACTCAGGCCAGGTTATCCCTCTCATTGTGGAAAGCTGCATCCGATTCATTAATCTCTATGGCCTTCAGCA CCAGGGGATTTTCAGAGTGTCTGGCTCCCAGGTGGAAGTGAACGATATCAAGAATTCTTTCGAGAGAGGT GAGAACCCTCTGTCCGATGAGCAGAGCAACCACGATATCAACTCGGTGGCCGGCGTGCTGAAGCTGTACT TCCGAGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGGTTTACTGACCTGATTTCCTGTATCAGAATAGA TAACCTCTATGAGAGGGCGCTCCACATCCGCAAACTCCTCCTGACCTTGCCCAGGTCGGTCCTTATAGTG ATGAGGTACCTGTTTGCCTTCCTCAATCACCTCTCCCAGTACAGTGACGAGAACATGATGGACCCTTACA ATCTGGCCATTTGCTTCGGCCCAACACTGATGCCGGTTCCAGAGATACAGGACCAAGTGTCTTGCCAGGC ACACGTGAACGAGATTGTGAAGACCATCATCATCCATCACGAGACTATTTTCCCAGATGCTAAAGAACTG GACGGCCCCGTGTATGAGAAATGCATGGCCGGAGGCGACTACTGCGACAGCCCCTACAGTGAGCATGGTA CATTGGAGGAGGTGGACCAAGACGCCGGCACAGAGCCCCACACCAGTGAAGACGAGTGTGAGCCCATCGA AGCCATAGCCAAGTTTGACTACGTCGGGCGGTCAGCTAGAGAGCTGTCCTTCAAGAAGGGTGCATCCCTG CTGCTGTATCACCGAGCCTCCGAGGATTGGTGGGAAGGCAGACACAACGGGATCGATGGCCTTGTGCCTC ATCAGTACATAGTTGTGCAAGATATGGACGATACATTTTCAGACACGCTGAGCCAAAAGGCCGACAGCGA AGCCAGCAGCGGGCCAGTCACTGAAGACAAGTCCTCATCCAAGGACATGAACTCCCCGACTGACCGCCAT TCTGATAGCTATTTAGCCAGACAAAGAAAGAGAGGAGAGCCACCCCCTCCGGGAAGACGTCCCGGCAGGA CCAGCGATGGCCATTGTCCCCTGCATCCCCCGCATGCTCTGTCTAACTCCTCAATTGATCTGGGATCCCC AAACCTAGCCAGTCACCCCAGGGGCCTGTTGCAGAACCGTGGCCTCAATAATGACAGTCCTGAGAGGCGG CGCCGGCCGGGCCACGGCAGCCTGACCAATATCAGCCGGCATGACTCCCTCAAGAAGATCGACAGCCCTC CTATCCGGAGGTCCACATCATCAGGGCAGTACACAGGCTTCAATGACCACAAGCCACTGGACCCCGAGAC GATTGCTCAGGATATTGAGGAGACCATGAACACTGCTTTGAACGAACTCCGTGAACTCGAGAGGCAGAGC ACAGTGAAGCACGCCCCCGATGTCGTGCTAGACACTTTGGAGCAAGTGAAGAACTCGCCCACCCCTGCGA CTTCCACAGAATCACTCAGCCCACTGCACAATGTCGCCCTCAGGGGCTCAGAGCCACAGATTCGCCGGAG CACCAGCTCCTCCAGCGAGACAATGAGTACTTTCAAGCCTATGGTGGCGCCCCGGATGGGCGTCCAGCTG AAGCCCCCAGCCCTTAGGCCAAAGCCTGCTGTTCTTCCAAAAACAAACCCTACGATGGGACCTGCCGCAC CTTCCCAGGGTCCCACAGACAAGTCTTGCACGATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224537 representing NM_001081037
Red=Cloning site Green=Tags(s) MSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEKL AERFMAKTRSTKDHQQFKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMF KKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHSESISAESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQ RRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRA LRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQQP VQAELMLRNQQLQSRLATLKIESEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYL SKPSIAKRRANQQETEQFYFMKLREFLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTTRPPNVPPKPQKH RKSRPRSQYNAKLFNGDLETFVKDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERG ENPLSDEQSNHDINSVAGVLKLYFRGLENPLFPKERFTDLISCIRIDNLYERALHIRKLLLTLPRSVLIV MRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIVKTIIIHHETIFPDAKEL DGPVYEKCMAGGDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELSFKKGASL LLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTDRH SDSYLARQRKRGEPPPPGRRPGRTSDGHCPLHPPHALSNSSIDLGSPNLASHPRGLLQNRGLNNDSPERR RRPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQS TVKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRGSEPQIRRSTSSSSETMSTFKPMVAPRMGVQL KPPALRPKPAVLPKTNPTMGPAAPSQGPTDKSCTM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081037 |
| ORF Size | 3255 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081037.2, NP_001074506.1 |
| RefSeq Size | 3414 bp |
| RefSeq ORF | 3258 bp |
| Locus ID | 117600 |
| Gene Summary | GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223490 | Srgap1 (untagged) - Mouse SLIT-ROBO Rho GTPase activating protein 1 (Srgap1), (10ug) |
CNY 13,870.00 |
|
| MR224537 | Srgap1 (Myc-DDK-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 1 (Srgap1) |
CNY 7,552.00 |
|
| MR224537L3 | Lenti ORF clone of Srgap1 (Myc-DDK-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 1 (Srgap1) |
CNY 11,400.00 |
|
| MR224537L4 | Lenti ORF clone of Srgap1 (mGFP-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 1 (Srgap1) |
CNY 11,400.00 |
