Cldn16 (NM_053241) Mouse Tagged ORF Clone
CAT#: MG225210
- TrueORF®
Cldn16 (tGFP-tagged) - Mouse claudin 16 (Cldn16), (10ug)
ORF Plasmid: DDK
"NM_053241" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | claudi; claudin-16; PC; PCLN1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225210 representing NM_053241
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGATCTTCTTCAGTACGCTGCCTGCTTCTTGGCCATATTCTCCACTGGGTTTTTGATCGTGGCCA CCTGGACAGACTGTTGGATGGTGAACGCTGATGACTCCCTGGAGGTGAGCACTAAATGCAGAGGCCTGTG GTGGGAGTGTGTAACAAACGCTTTTGATGGGATTCGAACCTGCGATGAGTACGACTCCATATATGCAGAA CATCCCTTGAAGCTGGTGGTAACTCGAGCACTGATGATCACAGCTGACATTTTAGCTGGCTTTGGATTCA TCACCCTGCTCCTTGGTCTGGACTGTGTGAAGTTCCTACCTGATGACCCACAAATTAAAGTCCGCCTTTG CTTTGTTGCAGGGACCACATTACTCATTGCAGGTACCCCAGGAATCATCGGTTCTGTGTGGTATGCTGTG GATGTTTACGTCGAACGCTCCTCTCTCGTTTTACACAATATATTTCTTGGGATCCAATATAAATTTGGTT GGTCCTGCTGGCTTGGAATGGCTGGGTCTTTGGGTTGCTTTTTGGCAGGAGCTCTCCTCACCTGCTGTTT GTACCTCTTCAAAGATGTTGGGCCTGAGAGGAACTACCCTTATGCCATGAGGAAGCCCTATTCAACTGCA GGTGTGTCCATGGCCAAGTCCTACAAGGCCCCTCGGACAGAGACAGCCAAAATGTATGCTGTAGACACCA GAGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225210 representing NM_053241
Red=Cloning site Green=Tags(s) MKDLLQYAACFLAIFSTGFLIVATWTDCWMVNADDSLEVSTKCRGLWWECVTNAFDGIRTCDEYDSIYAE HPLKLVVTRALMITADILAGFGFITLLLGLDCVKFLPDDPQIKVRLCFVAGTTLLIAGTPGIIGSVWYAV DVYVERSSLVLHNIFLGIQYKFGWSCWLGMAGSLGCFLAGALLTCCLYLFKDVGPERNYPYAMRKPYSTA GVSMAKSYKAPRTETAKMYAVDTRV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053241 |
ORF Size | 705 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053241.5, NP_444471.1 |
RefSeq Size | 1173 bp |
RefSeq ORF | 708 bp |
Locus ID | 114141 |
UniProt ID | Q925N4 |
Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is critical for renal paracellular epithelial transport of Ca(2+) and Mg(2+) in the thick ascending loop of Henle. The gene deficiency leads to specific alterations in renal Ca(2+) and Mg(2+) balance and also to disturbances in Na(+) handling. The interaction of this gene and the Cldn 19 gene is required for their assembly into tight junctions and for renal Mg(2+) reabsorption. This gene and the Cldn1 gene are clustered on chromosome 16. [provided by RefSeq, Aug 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212266 | Cldn16 (untagged) - Mouse claudin 16 (Cldn16), (10ug) |
CNY 3,990.00 |
|
MR225210 | Cldn16 (Myc-DDK-tagged) - Mouse claudin 16 (Cldn16) |
CNY 2,081.00 |
|
MR225210L3 | Lenti ORF clone of Cldn16 (Myc-DDK-tagged) - Mouse claudin 16 (Cldn16) |
CNY 4,750.00 |
|
MR225210L4 | Lenti ORF clone of Cldn16 (mGFP-tagged) - Mouse claudin 16 (Cldn16) |
CNY 4,750.00 |