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Epha2 (NM_010139) Mouse Tagged ORF Clone
CAT＃: MG226151
- TrueORF®
Epha2 (tGFP-tagged) - Mouse Eph receptor A2 (Epha2), (10ug)
ORF Plasmid: DDK
"NM_010139" in other vectors (4)
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CNY 9,410.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW545284; Eck; Myk2; Sek-2; Sek2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226151 representing NM_010139
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCCGGGCAGTCGGTTTCTGCCTGGCGCTGCTGTGGGGTTGCGCGCTGGCGGCCGCGGCGGCAC AGGGAAAGGAAGTTGTTTTGTTGGACTTCGCAGCAATGAAGGGAGAGCTCGGCTGGCTCACGCACCCCTA TGGCAAAGGGTGGGACCTGATGCAGAACATCATGGACGACATGCCTATCTACATGTACTCGGTGTGCAAC GTGGTATCCGGCGACCAGGACAACTGGCTCCGCACCAACTGGGTGTACCGGGAGGAGGCCGAGCGCATCT TTATTGAGCTCAAGTTCACGGTGCGAGACTGTAACAGCTTCCCGGGTGGCGCCAGCTCATGCAAAGAGAC CTTCAACCTCTACTATGCAGAGTCAGATGTGGACTATGGCACCAACTTCCAGAAGCGCCAGTTCACCAAG ATTGACACCATCGCCCCTGACGAGATCACGGTCAGCAGTGACTTCGAGGCTCGCAATGTCAAGCTGAACG TAGAGGAGCGCATGGTGGGGCCCCTTACCCGGAAGGGCTTCTACCTGGCCTTCCAGGACATCGGCGCCTG CGTGGCGCTGCTCTCCGTTCGCGTCTACTACAAGAAGTGTCCCGAGATGCTGCAGAGCTTGGCTCGCTTC CCCGAGACCATTGCTGTCGCTGTCTCCGATACACAACCCCTGGCCACGGTGGCCGGTACCTGCGTGGACC ATGCCGTGGTGCCTTATGGGGGCGAGGGGCCTCTCATGCACTGCACGGTGGATGGCGAGTGGCTGGTGCC CATCGGGCAGTGCCTGTGCCAGGAAGGCTACGAGAAGGTCGAGGATGCCTGCCGAGCCTGTTCTCCAGGA TTCTTCAAGTCTGAGGCATCTGAGAGCCCTTGCCTGGAGTGTCCAGAGCATACCCTGCCATCCACAGAGG GTGCCACCTCCTGCCAGTGTGAAGAAGGCTATTTCAGGGCACCTGAGGACCCACTGTCCATGTCTTGCAC ACGTCCACCCTCTGCCCCCAACTACCTCACGGCCATTGGCATGGGTGCCAAAGTAGAACTGCGTTGGACA GCTCCCAAGGACACTGGTGGCCGCCAGGACATTGTCTACAGTGTCACTTGCGAACAGTGCTGGCCAGAGT CTGGCGAGTGTGGGCCCTGTGAGGCGAGCGTGCGCTATTCAGAACCTCCTCACGCCCTGACCCGCACGAG TGTGACAGTCAGTGACCTGGAGCCCCACATGAACTATACCTTCGCTGTCGAAGCACGCAATGGTGTCTCA GGCCTGGTGACTAGCCGAAGCTTCCGGACTGCCAGCGTCAGTATTAACCAAACAGAGCCCCCCAAAGTGA GGCTGGAGGACCGAAGCACCACCTCCCTGAGTGTCACCTGGAGCATCCCGGTGTCACAGCAGAGCCGTGT GTGGAAGTACGAAGTCACCTACCGCAAGAAGGGGGATGCCAACAGCTATAATGTGCGCCGCACGGAAGGC TTCTCCGTGACCCTGGATGACCTTGCTCCGGATACCACGTACCTGGTGCAGGTGCAGGCGCTGACGCAGG AGGGCCAGGGAGCCGGCAGCAAAGTGCACGAGTTCCAGACACTGTCCACGGAAGGATCTGCCAACATGGC GGTGATCGGCGGTGTGGCTGTAGGTGTTGTTTTGCTTCTGGTACTGGCAGGAGTTGGCCTCTTCATCCAT CGAAGGAGGAGGAACCTGCGGGCTCGCCAGTCCTCTGAGGATGTCCGTTTTTCCAAGTCAGAACAACTAA AGCCCCTGAAGACCTATGTGGATCCTCACACTTACGAAGACCCCAACCAGGCTGTACTCAAGTTTACCAC CGAGATCCACCCATCCTGTGTGGCAAGGCAGAAGGTCATTGGAGCAGGAGAGTTTGGAGAGGTCTATAAA GGGACGCTGAAGGCATCCTCGGGGAAGAAGGAGATACCGGTGGCCATCAAGACACTGAAAGCGGGCTACA CTGAGAAGCAGCGGGTGGACTTCCTGAGCGAGGCCAGCATCATGGGCCAGTTTAGCCACCACAATATCAT CCGCCTGGAGGGCGTGGTCTCTAAATACAAACCCATGATGATTATCACAGAGTACATGGAGAATGGAGCG CTAGACAAGTTCCTTAGGGAGAAGGATGGTGAGTTCAGTGTACTTCAGTTGGTGGGCATGCTGAGGGGTA TCGCATCCGGCATGAAGTACCTGGCCAACATGAACTACGTGCACCGGGACCTGGCCGCCCGCAACATCCT CGTCAACAGCAACCTGGTGTGCAAGGTGTCCGATTTTGGCCTGTCGCGTGTGCTGGAAGATGACCCCGAG GCCACCTACACCACAAGTGGCGGCAAGATCCCTATTCGATGGACAGCCCCAGAGGCCATTTCCTACCGCA AGTTCACCTCAGCCAGCGATGTGTGGAGCTACGGCATTGTCATGTGGGAAGTGATGACTTATGGCGAACG GCCCTACTGGGAACTGTCAAACCACGAGGTCATGAAAGCCATCAACGACGGCTTCCGGCTCCCTACGCCC ATGGACTGCCCTTCAGCCATTTACCAGCTCATGATGCAGTGCTGGCAGCAAGAGCGCTCCCGCCGACCCA AGTTTGCCGACATCGTTAGCATCCTGGACAAGCTCATCCGAGCCCCCGACTCCCTCAAGACGCTGGCTGA CTTTGATCCCCGAGTGTCCATCCGGCTGCCCAGCACCAGCGGCTCGGAGGGAGTCCCCTTCCGTACGGTG TCCGAGTGGCTGGAGAGCATCAAGATGCAACAGTACACGGAACACTTCATGGTGGCTGGCTACACGGCCA TCGAGAAGGTGGTACAGATGTCCAACGAAGACATCAAAAGGATCGGAGTGCGTCTTCCTGGCCACCAGAA GCGTATTGCCTACAGCCTGCTGGGACTCAAGGACCAGGTCAACACAGTGGGGATTCCTATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226151 representing NM_010139
Red=Cloning site Green=Tags(s) MELRAVGFCLALLWGCALAAAAAQGKEVVLLDFAAMKGELGWLTHPYGKGWDLMQNIMDDMPIYMYSVCN VVSGDQDNWLRTNWVYREEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDVDYGTNFQKRQFTK IDTIAPDEITVSSDFEARNVKLNVEERMVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPEMLQSLARF PETIAVAVSDTQPLATVAGTCVDHAVVPYGGEGPLMHCTVDGEWLVPIGQCLCQEGYEKVEDACRACSPG FFKSEASESPCLECPEHTLPSTEGATSCQCEEGYFRAPEDPLSMSCTRPPSAPNYLTAIGMGAKVELRWT APKDTGGRQDIVYSVTCEQCWPESGECGPCEASVRYSEPPHALTRTSVTVSDLEPHMNYTFAVEARNGVS GLVTSRSFRTASVSINQTEPPKVRLEDRSTTSLSVTWSIPVSQQSRVWKYEVTYRKKGDANSYNVRRTEG FSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSTEGSANMAVIGGVAVGVVLLLVLAGVGLFIH RRRRNLRARQSSEDVRFSKSEQLKPLKTYVDPHTYEDPNQAVLKFTTEIHPSCVARQKVIGAGEFGEVYK GTLKASSGKKEIPVAIKTLKAGYTEKQRVDFLSEASIMGQFSHHNIIRLEGVVSKYKPMMIITEYMENGA LDKFLREKDGEFSVLQLVGMLRGIASGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPE ATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSYGIVMWEVMTYGERPYWELSNHEVMKAINDGFRLPTP MDCPSAIYQLMMQCWQQERSRRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTV SEWLESIKMQQYTEHFMVAGYTAIEKVVQMSNEDIKRIGVRLPGHQKRIAYSLLGLKDQVNTVGIPI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010139 |
| ORF Size | 2931 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010139.3, NP_034269.2 |
| RefSeq Size | 3948 bp |
| RefSeq ORF | 2934 bp |
| Locus ID | 13836 |
| UniProt ID | Q03145 |
| Gene Summary | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells (PubMed:29749928). Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222892 | Epha2 (untagged) - Mouse Eph receptor A2 (Epha2), (10ug) |
CNY 7,160.00 |
|
| MR226151 | Epha2 (Myc-DDK-tagged) - Mouse Eph receptor A2 (Epha2) |
CNY 7,152.00 |
|
| MR226151L3 | Lenti ORF clone of Epha2 (Myc-DDK-tagged) - Mouse Eph receptor A2 (Epha2) |
CNY 10,450.00 |
|
| MR226151L4 | Lenti ORF clone of Epha2 (mGFP-tagged) - Mouse Eph receptor A2 (Epha2) |
CNY 10,450.00 |
