Psmc2 (NM_011188) Mouse Tagged ORF Clone

CAT#: MG227108

  • TrueORF®

Psmc2 (tGFP-tagged) - Mouse proteasome (prosome macropain) 26S subunit ATPase 2 (Psmc2), (10ug)

ORF Plasmid: DDK tGFP



  "NM_011188" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,230.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG227108 representing NM_011188
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAGGGAACCGGCGCCCGGGCGCGGAGCTACTTCCGGTGGGGAAGAGAAAGGGAAGACACCAGCGG
AAGCGGGAAAGGTGCTGTGTAATCACCGAGACGCGGAGGCACTTGCGGCTTCTAAAATGCCGGATTACCT
AGGTGCGGACCAGCGGAAAACCAAAGAGGAGGAGAAGGACGACAAGCCCATCCGAGCTCTGGATGAAGGG
GATATTGCCTTGCTGAAAACTTACGGCCAAAGTACTTATTCAAGGCAGATCAAGCAGGTTGAAGATGACA
TTCAACAACTTCTCAAAAAAATTAATGAGCTCACTGGTATTAAAGAGTCTGACACTGGGTTGGCACCTCC
GGCCCTCTGGGATTTGGCTGCAGACAAACAAACACTTCAGAGTGAACAGCCATTACAGGTGGCAAGATGT
ACGAAGATAATCAACGCTGATTCGGAGGACCCAAAATACATCATCAATGTGAAGCAGTTTGCCAAGTTCG
TGGTGGATCTCAGTGATCAGGTGGCACCCACTGACATTGAAGAAGGGATGAGAGTCGGTGTGGACAGAAA
TAAATACCAAATTCACATTCCACTACCTCCTAAGATCGACCCAACAGTTACCATGATGCAGGTGGAGGAA
AAACCCGACGTCACATACAGTGATGTTGGTGGCTGTAAGGAACAGATTGAGAAATTGCGAGAAGTAGTTG
AAACCCCTCTGCTCCATCCAGAGAGGTTTGTTAACCTTGGAATCGAGCCTCCAAAGGGTGTACTGCTGTT
TGGGCCACCAGGCACAGGCAAGACACTCTGTGCTAGGGCAGTTGCCAATCGGACTGATGCTTGCTTCATT
CGAGTTATTGGGTCTGAGCTCGTACAGAAATACGTCGGCGAGGGAGCTCGAATGGTTCGTGAGCTTTTTG
AAATGGCCAGGACAAAAAAAGCCTGCCTTATTTTCTTTGATGAAATTGATGCCATTGGAGGGGCTCGGTT
TGACGATGGCGCTGGGGGTGACAATGAAGTGCAGAGAACCATGCTGGAGCTCATCAATCAGCTGGATGGC
TTTGATCCTCGCGGCAACATCAAGGTGCTCATGGCCACTAACCGCCCTGACACTCTGGACCCAGCGCTCA
TGAGACCGGGGAGGCTGGACAGGAAGATTGAGTTCAGCTTACCTGACTTAGAGGGTCGAACGCACATCTT
TAAGATTCACGCTCGCTCAATGAGTGTGGAAAGAGACATTCGATTTGAGCTGTTGGCCCGCCTGTGTCCA
AACAGTACTGGAGCGGAGATTAGAAGTGTTTGCACAGAAGCTGGTATGTTTGCAATCCGAGCACGGAGAA
AAATTGCTACAGAGAAGGACTTCTTAGAAGCTGTAAATAAGGTCATCAAGTCTTACGCCAAATTCAGCGC
TACTCCCCGCTACATGACATACAAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG227108 representing NM_011188
Red=Cloning site Green=Tags(s)

MSREPAPGRGATSGGEEKGKTPAEAGKVLCNHRDAEALAASKMPDYLGADQRKTKEEEKDDKPIRALDEG
DIALLKTYGQSTYSRQIKQVEDDIQQLLKKINELTGIKESDTGLAPPALWDLAADKQTLQSEQPLQVARC
TKIINADSEDPKYIINVKQFAKFVVDLSDQVAPTDIEEGMRVGVDRNKYQIHIPLPPKIDPTVTMMQVEE
KPDVTYSDVGGCKEQIEKLREVVETPLLHPERFVNLGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFI
RVIGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDG
FDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCP
NSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSYAKFSATPRYMTYN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011188
ORF Size 1425 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011188.3, NP_035318.1
RefSeq Size 1782 bp
RefSeq ORF 1428 bp
Locus ID 19181
UniProt ID P46471
Gene Summary Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC2 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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