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Tlr3 (NM_126166) Mouse Tagged ORF Clone
CAT＃: MG227542
- TrueORF®
Tlr3 (tGFP-tagged) - Mouse toll-like receptor 3 (Tlr3), (10ug)
ORF Plasmid: DDK
"NM_126166" in other vectors (4)
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CNY 9,980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI957183 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227542 representing NM_126166
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGGTGTTCCTCTTATCTAATGTACTCCTTTGGGGGACTTTTGTCCCTATGGATTCTTCTGGTGT CTTCCACAAACCAATGCACTGTGAGATACAACGTAGCTGACTGCAGCCATTTGAAGCTAACACACATACC TGATGATCTTCCCTCTAACATAACAGTGTTGAATCTTACTCACAACCAACTCAGAAGATTACCACCTACC AACTTTACAAGATACAGCCAACTTGCTATCTTGGATGCAGGATTTAACTCCATTTCAAAACTGGAGCCAG AACTGTGCCAAATACTCCCTTTGTTGAAAGTATTGAACCTGCAACATAATGAGCTCTCTCAGATTTCTGA TCAAACCTTTGTCTTCTGCACGAACCTGACAGAACTCGATCTAATGTCTAACTCAATACACAAAATTAAA AGCAACCCTTTCAAAAACCAGAAGAATCTAATCAAATTAGATTTGTCTCATAATGGTTTATCATCTACAA AGTTGGGAACGGGGGTCCAACTGGAGAACCTCCAAGAACTGCTCTTAGCAAAAAATGAAATCCTTGCGTT GCGAAGTGAAGAACTTGAGTTTCTTGGCAATTCTTCTTTACGAAAGTTGGACTTGTCATCAAATCCACTT AAAGAGTTCTCCCCGGGGTGTTTCCAGACAATTGGCAAGTTATTCGCCCTCCTCTTGAACAACGCCCAAC TGAACCCCCACCTCACAGAGAAGCTTTGCTGGGAACTTTCAAACACAAGCATCCAGAATCTCTCTCTGGC TAACAACCAGCTGCTGGCCACCAGCGAGAGCACTTTCTCTGGGCTGAAGTGGACAAATCTCACCCAGCTC GATCTTTCCTACAACAACCTCCATGATGTCGGCAACGGTTCCTTCTCCTATCTCCCAAGCCTGAGGTATC TGTCTCTGGAGTACAACAATATACAGCGTCTGTCCCCTCGCTCTTTTTATGGACTCTCCAACCTGAGGTA CCTGAGTTTGAAGCGAGCATTTACTAAGCAAAGTGTTTCACTTGCTTCACATCCCAACATTGACGATTTT TCCTTTCAATGGTTAAAATATTTGGAATATCTCAACATGGATGACAATAATATTCCAAGTACCAAAAGCA ATACCTTCACGGGATTGGTGAGTCTGAAGTACCTAAGTCTTTCCAAAACTTTCACAAGTTTGCAAACTTT AACAAATGAAACATTTGTGTCACTTGCTCATTCTCCCTTGCTCACTCTCAACTTAACGAAAAATCACATC TCAAAAATAGCAAATGGTACTTTCTCTTGGTTAGGCCAACTCAGGATACTTGATCTCGGCCTTAATGAAA TTGAACAAAAACTCAGCGGCCAGGAATGGAGAGGTCTGAGAAATATATTTGAGATCTACCTATCCTATAA CAAATACCTCCAACTGTCTACCAGTTCCTTTGCATTGGTCCCCAGCCTTCAAAGACTGATGCTCAGGAGG GTGGCCCTTAAAAATGTGGATATCTCCCCTTCACCTTTCCGCCCTCTTCGTAACTTGACCATTCTGGACT TAAGCAACAACAACATAGCCAACATAAATGAGGACTTGCTGGAGGGTCTTGAGAATCTAGAAATCCTGGA TTTTCAGCACAATAACTTAGCCAGGCTCTGGAAACGCGCAAACCCCGGTGGTCCCGTTAATTTCCTGAAG GGGCTGTCTCACCTCCACATCTTGAATTTAGAGTCCAACGGCTTAGATGAAATCCCAGTCGGGGTTTTCA AGAACTTATTCGAACTAAAGAGCATCAATCTAGGACTGAATAACTTAAACAAACTTGAACCATTCATTTT TGATGACCAGACATCTCTAAGGTCACTGAACCTCCAGAAGAACCTCATAACATCTGTTGAGAAGGATGTT TTCGGGCCGCCTTTTCAAAACCTGAACAGTTTAGATATGCGCTTCAATCCGTTCGACTGCACGTGTGAAA GTATTTCCTGGTTTGTTAACTGGATCAACCAGACCCACACTAATATCTCTGAGCTGTCCACTCACTACCT CTGTAACACTCCACATCATTATTATGGCTTCCCCCTGAAGCTTTTCGATACATCATCCTGTAAAGACAGC GCCCCCTTTGAACTCCTCTTCATAATCAGCACCAGTATGCTCCTGGTTTTTATACTTGTGGTACTGCTCA TTCACATCGAGGGCTGGAGGATCTCTTTTTACTGGAATGTTTCAGTGCATCGGATTCTTGGTTTCAAGGA AATAGACACACAGGCTGAGCAGTTTGAATATACAGCCTACATAATTCATGCCCATAAAGACAGAGACTGG GTCTGGGAACATTTCTCCCCAATGGAAGAACAAGACCAATCTCTCAAATTTTGCCTAGAAGAAAGGGACT TTGAAGCAGGCGTCCTTGGACTTGAAGCAATTGTTAATAGCATCAAAAGAAGCCGAAAAATCATTTTCGT TATCACACACCATTTATTAAAAGACCCTCTGTGCAGAAGATTCAAGGTACATCACGCAGTTCAGCAAGCT ATTGAGCAAAATCTGGATTCAATTATACTGATTTTTCTCCAGAATATTCCAGATTATAAACTAAACCATG CACTCTGTTTGCGAAGAGGAATGTTTAAATCTCATTGCATCTTGAACTGGCCAGTTCAGAAAGAACGGAT AAATGCCTTTCATCATAAATTGCAAGTAGCACTTGGATCTCGGAATTCAGCACAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227542 representing NM_126166
Red=Cloning site Green=Tags(s) MKGCSSYLMYSFGGLLSLWILLVSSTNQCTVRYNVADCSHLKLTHIPDDLPSNITVLNLTHNQLRRLPPT NFTRYSQLAILDAGFNSISKLEPELCQILPLLKVLNLQHNELSQISDQTFVFCTNLTELDLMSNSIHKIK SNPFKNQKNLIKLDLSHNGLSSTKLGTGVQLENLQELLLAKNEILALRSEELEFLGNSSLRKLDLSSNPL KEFSPGCFQTIGKLFALLLNNAQLNPHLTEKLCWELSNTSIQNLSLANNQLLATSESTFSGLKWTNLTQL DLSYNNLHDVGNGSFSYLPSLRYLSLEYNNIQRLSPRSFYGLSNLRYLSLKRAFTKQSVSLASHPNIDDF SFQWLKYLEYLNMDDNNIPSTKSNTFTGLVSLKYLSLSKTFTSLQTLTNETFVSLAHSPLLTLNLTKNHI SKIANGTFSWLGQLRILDLGLNEIEQKLSGQEWRGLRNIFEIYLSYNKYLQLSTSSFALVPSLQRLMLRR VALKNVDISPSPFRPLRNLTILDLSNNNIANINEDLLEGLENLEILDFQHNNLARLWKRANPGGPVNFLK GLSHLHILNLESNGLDEIPVGVFKNLFELKSINLGLNNLNKLEPFIFDDQTSLRSLNLQKNLITSVEKDV FGPPFQNLNSLDMRFNPFDCTCESISWFVNWINQTHTNISELSTHYLCNTPHHYYGFPLKLFDTSSCKDS APFELLFIISTSMLLVFILVVLLIHIEGWRISFYWNVSVHRILGFKEIDTQAEQFEYTAYIIHAHKDRDW VWEHFSPMEEQDQSLKFCLEERDFEAGVLGLEAIVNSIKRSRKIIFVITHHLLKDPLCRRFKVHHAVQQA IEQNLDSIILIFLQNIPDYKLNHALCLRRGMFKSHCILNWPVQKERINAFHHKLQVALGSRNSAH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_126166 |
| ORF Size | 2715 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_126166.4, NP_569054.2 |
| RefSeq Size | 4327 bp |
| RefSeq ORF | 2718 bp |
| Locus ID | 142980 |
| UniProt ID | Q99MB1 |
| Gene Summary | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222546 | Tlr3 (untagged) - Mouse toll-like receptor 3 (Tlr3), (10ug) |
CNY 11,690.00 |
|
| MR227542 | Tlr3 (Myc-DDK-tagged) - Mouse toll-like receptor 3 (Tlr3) |
CNY 9,944.00 |
|
| MR227542L3 | Lenti ORF clone of Tlr3 (Myc-DDK-tagged) - Mouse toll-like receptor 3 (Tlr3) |
CNY 10,930.00 |
|
| MR227542L4 | Lenti ORF clone of Tlr3 (mGFP-tagged) - Mouse toll-like receptor 3 (Tlr3) |
CNY 10,930.00 |
