Pdcd10 (NM_019745) Mouse Tagged ORF Clone

CAT#: MR202306

  • TrueORF®

Pdcd10 (Myc-DDK-tagged) - Mouse programmed cell death 10 (Pdcd10)

ORF Plasmid: DDK tGFP



  "NM_019745" in other vectors (6)

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Mouse Protein / Lysate Request

CNY 1,416.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2410003B13Rik; Ccm3; Tfa15; Tfar15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202306 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGATGACAATGGAAGAGATGAAGAATGAAGCTGAGACCACTTCCATGGTTTCTATGCCTCTCTATG
CAGTCATGTATCCTGTGTTTAATGAGCTAGAACGAGTAAATTTGTCTGCAGCTCAGACTCTGAGAGCCGC
TTTCATTAAGGCTGAAAAGGAAAATCCAGGCCTCACACAAGACATCATTATGAAAATATTAGAGAAAAAA
AGTGTAGAAGTTAACTTCACCGAGTCCCTCCTTCGTATGGCAGCTGATGACGTAGAAGAGTACATGATTG
AGCGGCCAGAGCCAGAATTCCAGGACCTAAATGAAAAGGCACGGGCACTTAAACAGATTCTCAGTAAGAT
TCCCGATGAGATCAATGACAGAGTGAGGTTTCTGCAGACAATCAAGGATATAGCTAGTGCAATAAAAGAA
CTACTTGATACAGTGAATAATGTCTTCAAAAAATATCAGTACCAGAACCGCAGGGCACTTGAACACCAAA
AGAAAGAATTTGTAAAATACTCCAAAAGTTTCAGTGACACTCTGAAGACTTATTTTAAAGATGGCAAGGC
AATAAATGTGTTCATAAGTGCCAACCGGCTAATTCATCAAACCAACTTGATACTTCAGACCTTCAAAACT
GTGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR202306 protein sequence
Red=Cloning site Green=Tags(s)

MRMTMEEMKNEAETTSMVSMPLYAVMYPVFNELERVNLSAAQTLRAAFIKAEKENPGLTQDIIMKILEKK
SVEVNFTESLLRMAADDVEEYMIERPEPEFQDLNEKARALKQILSKIPDEINDRVRFLQTIKDIASAIKE
LLDTVNNVFKKYQYQNRRALEHQKKEFVKYSKSFSDTLKTYFKDGKAINVFISANRLIHQTNLILQTFKT
VA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019745
ORF Size 639 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019745.1, NM_019745.2, NM_019745.3, NM_019745.4, NP_062719.2
RefSeq Size 1923 bp
RefSeq ORF 639 bp
Locus ID 56426
UniProt ID Q8VE70
MW 24.7 kDa
Gene Summary Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and STK26 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex (By similarity). Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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