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Hs6st1 (NM_015818) Mouse Tagged ORF Clone
CAT＃: MR206292
- TrueORF®
Hs6st1 (Myc-DDK-tagged) - Mouse heparan sulfate 6-O-sulfotransferase 1 (Hs6st1)
"NM_015818" in other vectors (3)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6Ost1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206292 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGAGCGCGCCAGCAAGTTCGTGCTGGTGGTGGCGGGCTCGGCGTGCTTCATGCTCATCCTTTACC AGTACGCGGGCCCGGGGCTGAGTCTGGGCGCGCCCGGTGGCCGCGTGCCCCCCGACGACCTGGATCTCTT CCCCACGCCGGACCCACATTACGAGAAAAAGTACTACTTCCCGGTGCGCGAGCTGGAGCGCTCGCTGCGC TTCGACATGAAGGGCGACGACGTGATCGTCTTCCTGCACATCCAGAAGACCGGCGGCACCACCTTCGGCC GCCACCTAGTGCAGAACGTGCGCCTCGAGGTGCCCTGCGACTGTCGCCCGGGCCAGAAGAAGTGCACCTG CTATCGGCCCAATCGCCGCGAGACCTGGCTCTTCTCTCGCTTCTCCACGGGCTGGAGCTGCGGGCTGCAC GCTGACTGGACCGAACTCACCAACTGTGTGCCCGGTGTGCTAGACCGCCGCGACCCAGCAGGTCTGCGTT CGCCCAGAAAGTTCTACTACATCACCCTGCTGCGAGACCCCGTATCCCGCTACCTGAGTGAATGGCGACA TGTACAGCGTGGGGCCACGTGGAAGACCTCCTTGCACATGTGTGACGGGCGCACACCGACCCCAGAGGAG CTGCCGCCCTGCTACGAGGGCACAGACTGGTCGGGCTGCACGTTGCAGGAGTTCATGGATTGCCCCTATA ACCTGGCTAACAACCGGCAGGTGCGCATGCTGGCCGACCTCAGCCTGGTGGGCTGCTACAACCTATCTTT CATCCCCGAGAGCAAGCGGGCCCAGTTGCTGCTGGAGAGCGCCAAGAAGAACCTGCGAGGCATGGCCTTC TTCGGCCTCACTGAGTTCCAGCGCAAGACGCAGTACCTATTTGAGCGGACGTTCAACCTCAAGTTCATCC GGCCATTCATGCAATACAACAGCACGCGGGCGGGCGGTGTGGAGGTGGATGAGGACACTATCCGCCACAT CGAGGAGCTCAACGACCTGGACATGCAGCTGTATGACTATGCCAAGGACCTCTTTCAGCAGCGTTACCAG TACAAGAGACAGCTGGAGCGCAGGGAACAGCGCCTGCGCAATCGCGAAGAGCGCCTCCTGCACCGCTCCA AGGAAGCGCTGCCACGGGAGGACCCAGAAGAGCCGGGCCGTGTGCCCACCGAGGACTACATGAGCCATAT CATTGAGAAGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206292 protein sequence
Red=Cloning site Green=Tags(s) MVERASKFVLVVAGSACFMLILYQYAGPGLSLGAPGGRVPPDDLDLFPTPDPHYEKKYYFPVRELERSLR FDMKGDDVIVFLHIQKTGGTTFGRHLVQNVRLEVPCDCRPGQKKCTCYRPNRRETWLFSRFSTGWSCGLH ADWTELTNCVPGVLDRRDPAGLRSPRKFYYITLLRDPVSRYLSEWRHVQRGATWKTSLHMCDGRTPTPEE LPPCYEGTDWSGCTLQEFMDCPYNLANNRQVRMLADLSLVGCYNLSFIPESKRAQLLLESAKKNLRGMAF FGLTEFQRKTQYLFERTFNLKFIRPFMQYNSTRAGGVEVDEDTIRHIEELNDLDMQLYDYAKDLFQQRYQ YKRQLERREQRLRNREERLLHRSKEALPREDPEEPGRVPTEDYMSHIIEKW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015818 |
| ORF Size | 1206 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015818.2, NP_056633.2 |
| RefSeq Size | 3732 bp |
| RefSeq ORF | 1236 bp |
| Locus ID | 50785 |
| UniProt ID | Q9QYK5 |
| MW | 47.1 kDa |
| Gene Summary | 6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Critical for normal neuronal development where it may play a role in neuron branching. May also play a role in limb development. May prefer iduronic acid.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209866 | Hs6st1 (untagged) - Mouse heparan sulfate 6-O-sulfotransferase 1 (Hs6st1), (10ug) |
CNY 3,656.00 |
|
| MR206292L3 | Lenti ORF clone of Hs6st1 (Myc-DDK-tagged) - Mouse heparan sulfate 6-O-sulfotransferase 1 (Hs6st1) |
CNY 4,750.00 |
|
| MR206292L4 | Lenti ORF clone of Hs6st1 (mGFP-tagged) - Mouse heparan sulfate 6-O-sulfotransferase 1 (Hs6st1) |
CNY 4,750.00 |
