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Syt1 (NM_009306) Mouse Tagged ORF Clone
CAT＃: MR206688
- TrueORF®
Syt1 (Myc-DDK-tagged) - Mouse synaptotagmin I (Syt1)
ORF Plasmid: tGFP
"NM_009306" in other vectors (3)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW124717; G630098F17Rik; SytI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206688 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAGTGCCAGTCGTCCTGAGGCCCTGGCTGCCCCTGTCACCACTGTTGCGACCCTTGTCCCACACA ACGCCACTGAGCCAGCCAGTCCTGGGGAAGGGAAGGAAGATGCCTTTTCCAAGCTGAAGCAGAAGTTTAT GAATGAACTGCATAAAATCCCATTGCCACCGTGGGCCTTAATTGCCATAGCCATAGTTGCGGTCCTTCTA GTCGTGACCTGCTGCTTCTGTGTCTGTAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAGGGAAAGG AAAAGGGAGGGAAGAACGCCATTAACATGAAAGACGTGAAAGACTTAGGGAAGACCATGAAGGATCAGGC CCTTAAGGATGACGATGCTGAAACTGGACTGACTGATGGAGAAGAAAAGGAGGAGCCCAAGGAAGAGGAG AAACTGGGAAAGCTTCAATATTCACTGGACTATGACTTCCAGAATAACCAGCTGCTGGTGGGAATCATCC AGGCTGCTGAACTGCCCGCCCTGGACATGGGAGGCACATCTGATCCATACGTCAAAGTCTTCCTGCTGCC CGACAAAAAGAAGAAGTTTGAGACAAAAGTCCACCGGAAAACCCTCAATCCAGTCTTCAATGAACAGTTT ACTTTCAAGGTGCCATACTCGGAATTAGGTGGCAAGACACTGGTGATGGCTGTGTATGATTTTGACCGCT TCTCCAAGCACGACATCATTGGAGAGTTCAAAGTTCCTATGAACACCGTGGATTTTGGCCACGTCACCGA GGAGTGGCGCGATCTCCAGAGTGCTGAGAAAGAAGAGCAAGAGAAACTGGGTGACATCTGCTTCTCCCTC CGCTACGTCCCTACTGCCGGCAAGCTGACTGTTGTCATTCTGGAAGCCAAGAACCTGAAGAAGATGGATG TGGGTGGCTTATCTGATCCCTATGTAAAGATTCACCTGATGCAGAACGGCAAGAGACTGAAGAAGAAAAA GACAACGATTAAGAAGAACACACTTAACCCCTACTACAATGAGTCCTTCAGCTTTGAAGTTCCGTTCGAG CAAATCCAGAAAGTGCAAGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGACGCCATCG GCAAAGTCTTTGTGGGCTACAACAGCACCGGCGCAGAGCTGCGACACTGGTCAGACATGCTGGCCAACCC CCGGCGACCCATCGCCCAGTGGCACACTCTGCAGGTAGAGGAGGAGGTTGATGCCATGCTGGCTGTCAAG AAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206688 protein sequence
Red=Cloning site Green=Tags(s) MVSASRPEALAAPVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALIAIAIVAVLL VVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEE KLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQF TFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSL RYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFE QIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVK K TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009306 |
| ORF Size | 1266 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009306.3 |
| RefSeq Size | 4756 bp |
| RefSeq ORF | 1266 bp |
| Locus ID | 20979 |
| UniProt ID | P46096 |
| MW | 47.4 kDa |
| Gene Summary | Calcium sensor that participates in triggering neurotransmitter release at the synapse (PubMed:11242035). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (PubMed:7961887). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206688 | Syt1 (tGFP-tagged) - Mouse synaptotagmin I (Syt1) |
CNY 3,140.00 |
|
| MR206688L3 | Lenti ORF clone of Syt1 (Myc-DDK-tagged) - Mouse synaptotagmin I (Syt1) |
CNY 4,750.00 |
|
| MR206688L4 | Lenti ORF clone of Syt1 (mGFP-tagged) - Mouse synaptotagmin I (Syt1) |
CNY 4,750.00 |
