Arl13b (NM_026577) Mouse Tagged ORF Clone

CAT#: MR206808

  • TrueORF®

Arl13b (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 13B (Arl13b)

ORF Plasmid: DDK tGFP



  "NM_026577" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 3,330.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms A530097K21Rik; A930014M17Rik; Arl2l1; C530009C10Rik; hnn
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206808 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCAGTCTGATGGCCAACTGCTGCAACTTGTTCAAGCGGTGGCGGGAGCCTGTCAGAAAGGTGACAC
TTGTGATGGTCGGCCTTGATAATGCTGGTAAAACAGCCACAGCAAAGGGAATCCAAGGAGAGCATCCTGA
AGACGTAGCTCCCACTGTTGGCTTTTCTAAAATTGATCTGAGACAAGGAAAGTTCCAAGTTACCATCTTT
GACTTAGGAGGTGGAAAAAGAATTCGGGGAATTTGGAAGAATTATTATGCTGAATCCTATGGGGTAATAT
TTGTTGTGGATTCCAGTGATGAGGAGAGAATGGAAGAAACAAAGGAGACAATGTCCGAAGTGCTAAGACA
CCCGAGGATATCAGGAAAGCCTATATTGGTGCTGGCCAACAAGCAAGACAAGGAGGGGGCTTTAGGCGAA
GCGGATGTGATTGAGTGTCTCTCGCTGGAGAAGCTGGTCAACGAGCACAAGTGCCTGTGTCAGATCGAAC
CTTGTTCAGCAGTCTTGGGATATGGAAAGAAAATTGACAAGTCCATTAAAAAGGGGCTTTATTGGCTACT
GCATATCATTGCAAAGGACTTTGATGCCTTAAGTGAACGCATCCAGAAAGACACAACTGAACAGCGAGCT
CTTGAGGAACAAGAGAAACGTGAGAGGGCTGAACGAGTCCGGAAGTTAAGGGAAGAAAGAGAACGAGAGC
AGACGGAACTTGATGGGACCAGTGGTCTGGCTGAGATTGACTCAGGACCAGTTCTTGCGAATCCTTTCCA
ACCCATTGCAGCTGTAATCATTGAGAATGAAAAGAAGCAGGAAAAAGAGAAAAAGAAGCAGACCGTGGAG
AAGGACAGTGATGTTGGTCTCCTAGAACATAAAGTGGAGCCAGAGCAAGCAGCGCCTCAGAGTGAGGCAG
ATTGCTGCCTCCAAAACCCTGACGAGCGAGTGGTGGACAGTTACAGGGAGGCACTGTCGCAGCAGCTGGA
CAGTGAGGATGAGCAGGACCAGCGAGGGTCAGAGTCCGGGGAAAACAGTAAGAAGAAAACCAAGAAACTA
AGAATGAAAAGGAGTCAGCGGGTAGAACCAGTCAATACAGACGAGTCTACTCCAAAGAGTCCCACGCCTC
CCCAACCTCCCCCTCCTGTTGGCTGGGGAACCCCCAAAGTCACTAGACTTCCAAAACTTGAGCCTCTTGG
TGAAACACGTCACAATGATTTCTATGGGAAGCCGCTGCCTCCCCTGGCTGTGCGACAGAGACCTAACGGT
GATGCTCAGGACACGATCTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206808 protein sequence
Red=Cloning site Green=Tags(s)

MFSLMANCCNLFKRWREPVRKVTLVMVGLDNAGKTATAKGIQGEHPEDVAPTVGFSKIDLRQGKFQVTIF
DLGGGKRIRGIWKNYYAESYGVIFVVDSSDEERMEETKETMSEVLRHPRISGKPILVLANKQDKEGALGE
ADVIECLSLEKLVNEHKCLCQIEPCSAVLGYGKKIDKSIKKGLYWLLHIIAKDFDALSERIQKDTTEQRA
LEEQEKRERAERVRKLREEREREQTELDGTSGLAEIDSGPVLANPFQPIAAVIIENEKKQEKEKKKQTVE
KDSDVGLLEHKVEPEQAAPQSEADCCLQNPDERVVDSYREALSQQLDSEDEQDQRGSESGENSKKKTKKL
RMKRSQRVEPVNTDESTPKSPTPPQPPPPVGWGTPKVTRLPKLEPLGETRHNDFYGKPLPPLAVRQRPNG
DAQDTIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_026577
ORF Size 1284 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_026577.2, NP_080853.2
RefSeq Size 3541 bp
RefSeq ORF 1284 bp
Locus ID 68146
UniProt ID Q640N2
MW 48.1 kDa
Gene Summary Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling (PubMed:23817546). Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex (PubMed:23153492).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...