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Nek2 (NM_010892) Mouse Tagged Lenti ORF Clone
CAT＃: MR207063L3
- LentiORF®
Lenti ORF clone of Nek2 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2)
"NM_010892" in other vectors (4)
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CNY 5,230.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA617254; C77054 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR207063).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010892 |
| ORF Size | 1332 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010892.1, NP_035022.1 |
| RefSeq Size | 3218 bp |
| RefSeq ORF | 1332 bp |
| Locus ID | 18005 |
| UniProt ID | O35942 |
| Gene Summary | Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (By similarity). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (By similarity). Phosphorylates and activates NEK11 in G1/S-arrested cells. Involved in the regulation of centrosome disjunction (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203803 | Nek2 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2), (10ug) |
CNY 2,000.00 |
|
| MG207063 | Nek2 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2) |
CNY 3,710.00 |
|
| MR207063 | Nek2 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2) |
CNY 3,330.00 |
|
| MR207063L4 | Lenti ORF clone of Nek2 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2) |
CNY 5,230.00 |
