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Eomes (NM_010136) Mouse Tagged ORF Clone
CAT＃: MR210179
- TrueORF®
Eomes (Myc-DDK-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 1
ORF Plasmid: tGFP
"NM_010136" in other vectors (6)
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CNY 7,768.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C77258; TBR-2; Tbr2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210179 representing NM_010136
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTGGGAGAGCAGCTCCTGGTGAGCTCGGTGAACCTGCCCGGCGCGCACTTCTACTCGCTGGAGA GTGCTCGCGGAGGAGGAGGAGGAGGAGGCGGAGGAGGAGGGGGAGGCGGAGGGAGCGTCAGCCTCCTCCC CGGTGCTGCCCCCTCGCCCCAGAGGCTGGACTTAGACAAAGCGTCCAAGAAGTTTCCGGGCAGTCTCCCG TGCCAGGCGGGGAGCGCAGAACCCGCAGGCGCCGGCGCGGGGGCCCCCGCGGCCATGCTCAGTGACGCGG ACGCTGGGGACACCTTCGGGAGCACCTCGGCGGTGGCCAAGCCCGGGCCCCCGGACGGCCGCAAGGGCTC CCCGTGCGCGGAGGAGGAGCTGCCCTCCGCCGCCACCGCCGCGGCCACCGCGCGCTACTCCATGGACAGC CTGAGCTCCGAGCGCTACTACCTCCCGTCGCCGGGACCGCAGGGCTCCGAGCTCGCCGCGCCCTGCTCGC TCTTCCAGTACCCGGCGGCGGCCGGAGCAGCCCACGGACCCGTGTACCCCGCGTCCAATGGCGCGCGCTA CCCCTACGGCTCCATGCTGCCCCCCGGTGGATTCCCCGCCGCCGTGTGCCCGCCCGCGAGGGCGCAGTTC GGCCCCGCTGCGGGTTCGGGGAGCGGCGCTGGTAGCAGCGGCGGTGGTGCCGGCGGTCCTGGCGCCTATC CCTACGGCCAGGGTTCTCCGCTCTACGGGCCATACGCCGGAACCTCAGCGGCCGGGTCTTGTGGAGGATT GGGGGGCCTAGGGGTGCCTGGCTCCGGCTTCCGCGCCCACGTCTACCTGTGCAACCGGCCCCTATGGCTC AAATTCCACCGGCACCAAACTGAGATGATCATCACCAAACAGGGCAGGCGCATGTTTCCTTTCTTGAGCT TCAACATAAACGGACTCAACCCCACCGCCCACTACAATGTTTTCGTGGAAGTGGTTCTGGCCGACCCTAA CCACTGGCGCTTCCAGGGGGGCAAGTGGGTGACCTGCGGCAAAGCGGACAATAACATGCAGGGCAATAAG ATGTACGTTCACCCAGAATCTCCTAACACTGGCTCCCACTGGATGAGGCAGGAGATTTCCTTTGGGAAGT TAAAACTCACCAATAACAAAGGTGCAAACAACAACAACACACAGATGATAGTGTTGCAGTCTCTGCACAA ATACCAACCGAGGCTGCACATCGTGGAAGTGACAGAGGACGGTGTGGAGGACTTGAATGAACCTTCCAAG ACTCAGACCTTCACCTTCTCAGAGACACAGTTCATCGCTGTGACGGCCTACCAAAACACGGATATCACCC AGCTAAAGATCGACCATAACCCCTTCGCCAAAGGCTTCCGGGACAACTACGATTCCATGTACACGGCTTC AGAAAATGACAGGTTAACTCCATCTCCCACGGATTCCCCTAGATCCCATCAGATTGTCCCTGGAGGTCGG TACGGCGTTCAAAACTTCTTCCCGGAGCCCTTTGTCAACACTTTGCCTCAAGCCCGATATTATAACGGTG AGAGAACCGTGCCACAGACCAACGGCCTCCTCTCACCCCAACAGAGCGAAGAGGTGGCCAACCCTCCCCA GCGGTGGCTTGTCACGCCTGTCCAGCAACCTGTGACCAACAAGCTAGACATCGGTTCTTATGAATCTGAA TATACTTCCAGTACCTTGCTCCCATATGGTATTAAGTCCTTGCCCCTGCAGACATCCCATGCCCTGGGGT ATTACCCTGACCCGACCTTCCCTGCTATGGCAGGGTGGGGAGGCCGTGGCGCTTATCAGAGGAAGATGGC AGCTGGACTACCATGGACATCCAGAATGAGCCCACCTGTCTTCCCAGAAGATCAGCTTGCCAAGGAAAAA GTTAAAGAAGAGATTAGTTCCTCCTGGATAGAGACTCCCCCCTCCATCAAGTCTCTAGACTCCAGCGACT CCGGGGTGTACAACAGCGCTTGCAAGAGAAAGCGCCTGTCTCCCAGCACCCCCAGCAATGGAAACTCGCC CCCCATAAAGTGTGAGGACATTAACACTGAAGAGTACAGTAAAGACACCTCCAAAGGCATGGGGGCTTAT TATGCTTTTTACACAAGTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210179 representing NM_010136
Red=Cloning site Green=Tags(s) MQLGEQLLVSSVNLPGAHFYSLESARGGGGGGGGGGGGGGGSVSLLPGAAPSPQRLDLDKASKKFPGSLP CQAGSAEPAGAGAGAPAAMLSDADAGDTFGSTSAVAKPGPPDGRKGSPCAEEELPSAATAAATARYSMDS LSSERYYLPSPGPQGSELAAPCSLFQYPAAAGAAHGPVYPASNGARYPYGSMLPPGGFPAAVCPPARAQF GPAAGSGSGAGSSGGGAGGPGAYPYGQGSPLYGPYAGTSAAGSCGGLGGLGVPGSGFRAHVYLCNRPLWL KFHRHQTEMIITKQGRRMFPFLSFNINGLNPTAHYNVFVEVVLADPNHWRFQGGKWVTCGKADNNMQGNK MYVHPESPNTGSHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDLNEPSK TQTFTFSETQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTASENDRLTPSPTDSPRSHQIVPGGR YGVQNFFPEPFVNTLPQARYYNGERTVPQTNGLLSPQQSEEVANPPQRWLVTPVQQPVTNKLDIGSYESE YTSSTLLPYGIKSLPLQTSHALGYYPDPTFPAMAGWGGRGAYQRKMAAGLPWTSRMSPPVFPEDQLAKEK VKEEISSSWIETPPSIKSLDSSDSGVYNSACKRKRLSPSTPSNGNSPPIKCEDINTEEYSKDTSKGMGAY YAFYTSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010136 |
| ORF Size | 2121 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010136.3, NP_034266.2 |
| RefSeq Size | 3731 bp |
| RefSeq ORF | 2124 bp |
| Locus ID | 13813 |
| UniProt ID | O54839 |
| MW | 75.3 kDa |
| Gene Summary | Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The evolution of basal progenitors in the developing non-mammalian brain
,Nomura, T;Ohtaka-Maruyama, C;Yamashita, W;Wakamatsu, Y;Murakami, Y;Calegari, F;Suzuki, K;Gotoh, H;Ono, K;,
Development
,PubMed ID 26732839
[EOMES]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220687 | Eomes (untagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 1, (10ug) |
CNY 7,776.00 |
|
| MG210179 | Eomes (tGFP-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes) |
CNY 9,368.00 |
|
| MR210179L1 | Lenti ORF clone of Eomes (Myc-DDK-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 1 |
CNY 8,930.00 |
|
| MR210179L2 | Lenti ORF clone of Eomes (mGFP-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 1 |
CNY 10,168.00 |
|
| MR210179L3 | Lenti ORF clone of Eomes (Myc-DDK-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 1 |
CNY 8,930.00 |
|
| MR210179L4 | Lenti ORF clone of Eomes (mGFP-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 1 |
CNY 10,168.00 |
