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Cnot10 (NM_153585) Mouse Tagged ORF Clone
CAT＃: MR210418
- TrueORF®
Cnot10 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10)
ORF Plasmid: tGFP
"NM_153585" in other vectors (4)
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CNY 5,448.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2600001P13Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210418 representing NM_153585
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGACAAGCCTGCAGATCAGGGAGCAGAGAAGCATGAAGGGGCAGGCCAATCATCTGGGGTCA CGGACCAGGAGAAGGAGCTGTCCGCCAGTGCCCTCCAGGCCTTCACGTCTGGAAATTATGATGCCTGTCT GCAACACCTTGCCTGTCTCCAAGATATAAACAAAGATGATTACAAAATTATTCTGAATACAGCAGTAGCT GAATTTTTTAAAAATAATCAAACAACAACAGATAATTTGAGACAAACACTTAACCAGCTGAAGAATCAGG TCCACTCGGCTGTTGAAGAGATGGATGGGTTAGACGATGTTGAGAACAGCATGCTGTATTATAACCAGGC TGTCATTCTCTATCATCTGCGCCAGTACACGGAGGCCATATCTGTCGGCGAGAAGCTCTATCAGTTCATA GAACCTTTCGAAGAAAAATTTGCCCAAGCGGTGTGCTTTTTGCTCGTAGACTTGTATATATTAACCCACC AGGCTGAGAAAGCTCTGCATCTTCTTGCCGTCCTAGAAAAAATGATTTCACAGGGTAGCGGTGGCAAAAA TGGGAAGAACGAGACTGGCAATAACAGCAGCAAAGATGGATCCAACCCTAAGGCTGAGAGTGCAGCTCTA ATAGAGGCTGCCAAGTCCAAGATACACCAGTACAAAGTCAGAGGGTACATCCAGATGAAGTCCCTGAAGG CTTGCAAAAGGGAAATCAAGTCTGTCATGAACACAGCTGGAAATTCTGCACCTTCCCTGTTTCTTAAAAG CAACTTTGAGTACTTAAGGGGCAACTATCGAAAAGCGGTGAAGCTGTTGAATAGTTCAAACATTGCCGAG CATCCAGGCTTCATGAAGACAGGTGAATGCTTGAGATGCATGTTCTGGAATAACCTTGGTTGTATCCATT TTGCCATGAGCAAGCACAATTTGGGGATTTTCTACTTCAAGAAGGCTCTGCAGGAAAATGACAACGTCTG CGCCCAGCTCAGTGCGGGTGGCACTGATCCAGGCAAAAAGTTTTCTGGAAGACCCATGTGTACATTGCTG ACCAACAAAAGATATGAGCTGCTCTACAACTGTGGGATCCAGCTGCTGCACGTCGGGAGGCCTCTCGCTG CCTTCGAGTGTCTGATTGAAGCTGTTCAGGTGTACCACGCGAATCCTCGTCTCTGGCTACGGCTGGCTGA GTGCTGCATTGCTGCCAATAAAGGGACTTCTGAGCAAGAAACTAAAGGTCTTCCCACCAAAAAAGGCATT GTACAGTCTATTGTTGGTCAGGGATATCACCGTAAAATAGTTCTGGCATCACAGTCAATTCAGAACACTG TCTACAATGATGGTCAGTCTTCAGCCATTCCTGTAGCCAGTGTGGAGTTTGCAGCCATCTGTCTCAGAAA TGCCTTGTTGCTGTTACCTGAAGAACAGCAAGATCCCAAGCAGGAAAATGGCTCCAAGAGCAGCAGCCAG CTTGGAGGGAACACGGAAAGCAGCGAGAGCAGCGAGACGTGCAGCAGTAAGAGCCATGATGGAGATAAGT TCATTCCCGCTCCACCGTCTTCTCCGCTGAGGAAACAGGAGTTAGAAAACCTCAAGTGCTCCATCCTTGC TTGCAGTGCTTATGTGGCTCTGGCCTTAGGTGATAACCTCATGGCTTTGAACCATGCAGATCAGCTCCTG CAGCAGCCCAAGCTGTCTGGCTCTCTTAAATTTTTGGGCCATTTATATGCCGCAGAAGCCCTCATCTCCC TCGACAGGATATCTGATGCCATTACACACTTGAACCCAGAGAATGTCACTGATGTCTCCTTAGGGATCTC TTCAAATGAGCAGGACCAAGGATCAGACAAGGGTGAAAATGAAGCAATGGAATCCTCTGGCAAGCGCGCC CCTCAGTGCTACCCCAGTTCTGTGAACTCTGCCCGGACTGTGATGCTCTTCAACCTGGGCAGTGCCTACT GCCTGAGGAGCGAGTACGACAAGGCCCGAAAGTGTCTGCATCAGGCGGCATCGATGATCCATCCTAAGGA GGTGCCCCCTGAAGCCATCCTGCTGGCTGTCTACCTTGAGCTGCAGAATGGTAATACACAGCTGGCCTTG CAGATGATCAAGCGGAACCAGCTGCTCCCCGCAGTAAAGGCGCACTCTGATGTGAGGAAGAAGACAGTGT TCCAGCCCGTGCACCCCATCCAGCCCATCCAAATGCCAGCGTTCACCACCGTGCAGAGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210418 representing NM_153585
Red=Cloning site Green=Tags(s) MAADKPADQGAEKHEGAGQSSGVTDQEKELSASALQAFTSGNYDACLQHLACLQDINKDDYKIILNTAVA EFFKNNQTTTDNLRQTLNQLKNQVHSAVEEMDGLDDVENSMLYYNQAVILYHLRQYTEAISVGEKLYQFI EPFEEKFAQAVCFLLVDLYILTHQAEKALHLLAVLEKMISQGSGGKNGKNETGNNSSKDGSNPKAESAAL IEAAKSKIHQYKVRGYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSSNIAE HPGFMKTGECLRCMFWNNLGCIHFAMSKHNLGIFYFKKALQENDNVCAQLSAGGTDPGKKFSGRPMCTLL TNKRYELLYNCGIQLLHVGRPLAAFECLIEAVQVYHANPRLWLRLAECCIAANKGTSEQETKGLPTKKGI VQSIVGQGYHRKIVLASQSIQNTVYNDGQSSAIPVASVEFAAICLRNALLLLPEEQQDPKQENGSKSSSQ LGGNTESSESSETCSSKSHDGDKFIPAPPSSPLRKQELENLKCSILACSAYVALALGDNLMALNHADQLL QQPKLSGSLKFLGHLYAAEALISLDRISDAITHLNPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRA PQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLAL QMIKRNQLLPAVKAHSDVRKKTVFQPVHPIQPIQMPAFTTVQRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153585 |
| ORF Size | 2232 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153585.5, NP_705813.2 |
| RefSeq Size | 2990 bp |
| RefSeq ORF | 2235 bp |
| Locus ID | 78893 |
| UniProt ID | Q8BH15 |
| MW | 82.3 kDa |
| Gene Summary | Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206010 | Cnot10 (untagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10), (10ug) |
CNY 2,000.00 |
|
| MG210418 | Cnot10 (tGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10) |
CNY 6,370.00 |
|
| MR210418L3 | Lenti ORF clone of Cnot10 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10) |
CNY 7,700.00 |
|
| MR210418L4 | Lenti ORF clone of Cnot10 (mGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10) |
CNY 7,700.00 |
