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Smarcal1 (NM_018817) Mouse Tagged ORF Clone
CAT＃: MR211150
- TrueORF®
Smarcal1 (Myc-DDK-tagged) - Mouse SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1)
ORF Plasmid: tGFP
"NM_018817" in other vectors (4)
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CNY 6,664.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6030401P21Rik; AI851716; Mharp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211150 representing NM_018817
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTGCCACTTACAGAGGAGCAGAGGAAAAAGATTGAAGAGAATCGGCAAAAAGCTTTGGCCCGTC GAGCTGAGAAGTTGTCAGAACAGCCACAGAGCGCAGCTTCAGGCTCCTCTGCTGCCGGCCCGTCCCAGTC CAAGCAGGGCTCTCTCCTGAATCTCCTAGCGGAGCCTTCAAAGCCAGTGGGCCATGCCTCCATTTTCAAG CAACAGAATCTCAGTAATTCATTTCCTACTGATCAGAGACCTCACAGCTCCCGCTGTTCTCAGCCCAGCC CAGCAGAGGAAACGACGGGGCTATGGAAGACCCAAGGAGAGATGTCCACAGCCTGCCCAAAGCCTAACCC AAGTCCTCCAGGAGCCTCTAACCAACCACTTTTGGGTTATAAATCCAGCGAGGGTCAACCCCAGGCAACC TGGGACACAGGAGCTTCCTCTTCTGGACCATTCCCCAGGGACCCCGAGTTAGAGGCCAAGGCAGCAAGGC CTTCCACCTCTAGGCAGAGCATTTCGGACAGCTTTTATGTGTTAGGGGGAAAAACACCTAGGACAGAAGG GAGACCCCCAAACATACTGCAAACCACACCCCAAAACACAGGCTTTCTACGGGGAGCGTGCATAAAGACT GGGGATCGCTTCCGGGTAAAGATCGGCTACAACCAAGAGCTCATTGCCGTGTTTAAGTCTCTGCCCAGCA GACACTATGATTCTTTTACAAAGACCTGGGACTTCAGCATGAGCGACTACAGAGCCTTGATGAAGGCAGT CGAGCGACTCTCCACGGTCTCCCTGAAGCCACTGGACGAGGCTGGTGGCAGTGTTGGAGGACAGACCAGC CTTCCCTCAGCTCCATCCCTGACCTTTGTGACAGGGAAGTGCATGCTCATCTCTCGAGTCCGCTTCGAGG TTGACATCGGCTATTCGGAGGCGGTGATTGGCCTGTTTAAACAGATGGAGTCCAGGAGTTACGATATCAA GACCAGGAAGTGGAGTTTTCTCTTGGAAGAGCACAATAAACTAATTGCAAGGTCACGTGAACTGAAGCAA GTTCAGCTGGACCCACTGCCCAAGACAGTGACCCTGGCATTTGCATCTCAGCTGGAGAAAACGTCTCCCA AACTGAAAGCAGACGTGCCAGAAGCGGACCTTTCCGGAGTGGATGCCAAGCTTGTGTCTAGCCTCATGCC CTTTCAGAGGGAGGGAGTTAGCTTTGCCATATCAAAAAGAGGCCGCCTGCTACTTGCCGATGACATGGGC CTAGGGAAGACCGTCCAAGCCATCTGCATAGCAGCTTTTTACCGAAAGGAATGGCCACTGCTGGTGGTCG TGCCTTCCTCTGTGCGCTTCACCTGGGAGCAGGCCTTTCTTCGGTGGCTTCCATCTCTTAGCCCAGAGAA CATCAACGTGGTGGTGACCGGGAAGGGCCGCCTAACAGCTGGCTTGGTCAATATTGTCAGCTTTGACCTC CTTTGCAAGTTGGAGAGGCAGCTAAAAACCCCTTTCAAAGTCGTCATTATTGATGAATCACACTTCCTCA AGAACATAAAGACTGCCCGCTGCCGAGCAGCTGTGCCTATCCTAAAGGTTGCCAAGAGGGTGATCCTACT GTCAGGCACACCCGCCATGTCTCGGCCCGCAGAGCTCTACACGCAGATCATTGCTGTCAAGCCAACCTTC TTCCCTCAGTTTCATGCCTTTGGACTCCGCTACTGTGACGCCAAACGGCTCCCTTGGGGCTGGGACTACT CAGGCTCCTCCAACCTGGGCGAGCTGAAGCTGCTGCTAGAGGAAGCAATCATGCTGCGGCGCCTCAAATC CGACGTGCTCTCCCAGCTTCCGGCCAAGCAGCGCAAGATGGTGGTGGTCAACCCTGGTCGGATCAGCTCC AGGGCTAAGGCAGCCCTGGATGCAGCAGCCAAAGAAATGACCAAGGACAAAACTAAACAGCAACAGAAAG AAGCTCTCCTTGTCTTCTTCAACAGAACAGCAGAAGCTAAAATCCCATGTGTCGTCGAGTATATCCTGGA CCTTCTGGACAGCGGACGAGAGAAGTTTCTAGTGTTTGCACACCATAAGGTGATTCTGGATGCAGTTGCC AAGGAGCTCGAGAGGAAGAACGTTCAACACATCCGGATCGATGGCTCCACTCCCTCAGCAGACCGGGAGG CTCAGTGCCAGCGGTTCCAGCTGTCCAAAGGGCACACAGTGGCACTGTTGTCCATCACAGCCGCCAACAT GGGCCTCACCTTCTCCACAGCTGATCTGGTCGTGTTTGCTGAGCTCTTCTGGAACCCAGGGGTGCTGATC CAGGCCGAGGACCGTGTGCACCGCATTGGACAGACAAACTCTGTGAGCATCCACTACCTGGTGGCAAAAG GCACAGCAGATGACTACCTTTGGCCTCTGATTCAAGAGAAGATTAAAGTTCTGGGGGAAGCTGGGCTTTC TGAGACCAATTTCTCGGAAATGACGGAAGCTACAGACTACGTGCACAAGGACCCAAAGCAGAAGACGATC TATGACCTGTTCCAGCAGTCCTTTGAAGACGATGGAAATGACATGGAATTCCTGGAAGCAGCAGAGTCCT TTGAGCTAGGAAGTACTTCAGGAACCTCTGGAAACATTTCCCAGGATCTGGGAGATCTCTTAGATGAAGA CGAAGGCAGTCCGCCAAAGAAAAGCAGATTTGAATTCTTTGATAATTGGGACAGCTTTTCCTCTCCCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211150 representing NM_018817
Red=Cloning site Green=Tags(s) MSLPLTEEQRKKIEENRQKALARRAEKLSEQPQSAASGSSAAGPSQSKQGSLLNLLAEPSKPVGHASIFK QQNLSNSFPTDQRPHSSRCSQPSPAEETTGLWKTQGEMSTACPKPNPSPPGASNQPLLGYKSSEGQPQAT WDTGASSSGPFPRDPELEAKAARPSTSRQSISDSFYVLGGKTPRTEGRPPNILQTTPQNTGFLRGACIKT GDRFRVKIGYNQELIAVFKSLPSRHYDSFTKTWDFSMSDYRALMKAVERLSTVSLKPLDEAGGSVGGQTS LPSAPSLTFVTGKCMLISRVRFEVDIGYSEAVIGLFKQMESRSYDIKTRKWSFLLEEHNKLIARSRELKQ VQLDPLPKTVTLAFASQLEKTSPKLKADVPEADLSGVDAKLVSSLMPFQREGVSFAISKRGRLLLADDMG LGKTVQAICIAAFYRKEWPLLVVVPSSVRFTWEQAFLRWLPSLSPENINVVVTGKGRLTAGLVNIVSFDL LCKLERQLKTPFKVVIIDESHFLKNIKTARCRAAVPILKVAKRVILLSGTPAMSRPAELYTQIIAVKPTF FPQFHAFGLRYCDAKRLPWGWDYSGSSNLGELKLLLEEAIMLRRLKSDVLSQLPAKQRKMVVVNPGRISS RAKAALDAAAKEMTKDKTKQQQKEALLVFFNRTAEAKIPCVVEYILDLLDSGREKFLVFAHHKVILDAVA KELERKNVQHIRIDGSTPSADREAQCQRFQLSKGHTVALLSITAANMGLTFSTADLVVFAELFWNPGVLI QAEDRVHRIGQTNSVSIHYLVAKGTADDYLWPLIQEKIKVLGEAGLSETNFSEMTEATDYVHKDPKQKTI YDLFQQSFEDDGNDMEFLEAAESFELGSTSGTSGNISQDLGDLLDEDEGSPPKKSRFEFFDNWDSFSSPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018817 |
| ORF Size | 2730 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018817.2, NP_061287.2 |
| RefSeq Size | 6715 bp |
| RefSeq ORF | 2733 bp |
| Locus ID | 54380 |
| UniProt ID | Q8BJL0 |
| MW | 101.3 kDa |
| Gene Summary | ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201448 | Smarcal1 (untagged) - Mouse SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1), (10ug) |
CNY 2,000.00 |
|
| MG211150 | Smarcal1 (tGFP-tagged) - Mouse Swi/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1) |
CNY 7,890.00 |
|
| MR211150L3 | Lenti ORF clone of Smarcal1 (Myc-DDK-tagged) - Mouse SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1) |
CNY 9,030.00 |
|
| MR211150L4 | Lenti ORF clone of Smarcal1 (mGFP-tagged) - Mouse SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1) |
CNY 9,030.00 |
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