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Gba2 (NM_172692) Mouse Tagged ORF Clone
CAT＃: MR211175
- TrueORF®
Gba2 (Myc-DDK-tagged) - Mouse glucosidase beta 2 (Gba2)
ORF Plasmid: tGFP
"NM_172692" in other vectors (4)
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CNY 6,720.00
CNY 8,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | F630034E04 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211175 representing NM_172692
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAACCTGCGTCCCGGCCTCAGAGCAGGTCGGCTGTGCCGAAAGAGATTCCCAAGTTTATTGTGAAG ATACTGGCGGCACTGAGGCTGTGCGGGTTACAGACTGCGGGAGCCCCGAGGATAGTGGACCCCAGGATGA ACCAAGCTACTGCAATTCAGAGGACTCTGGGCAGCTGATGGCCTCCTACGAGGGTAAAGCTAGGGGCTAC CAGGTGCCTCCTTTTGGCTGGCGGATCTGCTTGGCTCACGAGTTTGCAGAGAAGAGGAGACCTTTTCAAG CTAACAACATCTCTCTCAGTAATTTGGTAAAGCACCTCGGTATGGGCTTGAGGTACTTGAAGTGGTGGTA CCGAAAGACCCACGTGGAAAAGAAGACCCCTTTCATCGACATGCTCAATTCTCTACCCCTGAGACAGATC TATGGTTGTCCCCTGGGTGGCATTGGAGGAGGCACTATCACCCGGGGCTGGAGAGGCCAGTTCTGTCGTT GGCAGCTCAACCCTGGAATGTACCAGCACCAGACAGTCATTGCAGACCAATTTATAGTATGCTTGCGTCG AGATGGGCGGACTGTGTACCAGCAAGTTCTGTCCTTGGAGCTTCCAAATGTCCTGCGCAGCTGGAACTGG GGCCTGTGTGGTTACTTTGCTTTCTACCACGCCCTCTATCCCCGAGCCTGGACGGTCTATCAGCTTCCTG GCCAGAATGTCACCCTCACCTGTCGCCAGGTCACACCTATCTTGCCTCATGACTACCAGGACAGCAGTCT CCCTGTAGGAGTCTTTGTGTGGGATGTAGAAAACGAAGGAGATGAGACTCTGGACGTTTCCATCACGTTC TCCATGCGGAATGGACTAGGAGGCGAAGATGATGCGGCCGGGAGTTTGTGGAATGAGCCCTTCCGCCTGG AACAGGGCGGGACGACTGTTCAGGGGCTCCTCTTGCATCATCCAACCCCTCCGAACCCCTACACCATGGC TGTGGCTGCACGATGCACGGCAGATACCACGGTAACCCACACCACAGCCTTTGACCCCAATGGCACTGGA CAGCAGGTGTGGCAGGACCTACTTCAGGATGGACAGCTGGACTCCCCTGCTGGCCAAAGCACCCCAACAC AGAAAGGAGAGGGTATCGCTGGGGCTGTATGCGTCTCTAGCAAGCTGTTACCTCGAAGCCGGTGCTGCTT GGAGTTCTCACTGGCTTGGGATATGCCTAAGATCATGTTTGGAGCTAAAAGCCAAGTCCACTACAGGCGG TATACACGGTTCTTTGGTTCAGATGGTGATGTGGCGCCTGCCCTGAGCCACTATGCACTGTGCCACTATG CAGACTGGGAGGACAGAATCTCAGCCTGGCAGAACCCAGTACTGGATGACAGAACCTTGCCTGCCTGGTA CAAATCTGCACTGTTCAATGAACTGTACTTCCTGGCCGATGGAGGCACAGTGTGGCTGGAAGTTCCCGCA GACTCCCTACCAGAGGGGCTGGGAGGGAGTATGCGTCAGCTTCGCTCTACTCTGCAGGACTATGGGCGAT TTGGCTATCTTGAAGGCCAGGAGTACCGCATGTACAACACATACGATGTCCACTTTTATGCGTCCTTTGC CCTCGTCATGCTGTGGCCCAAACTTGAGCTCAGTCTTCAGTATGATATGGCTCTGGCAACTTTGAAGGAG GACCTGACCCGGCGACGGTACCTGATGAGTGGAGTGGTGGCACCTGTGAAAAGGAGGAACGTCATCCCTC ATGACATTGGGGATCCGGATGATGAGCCATGGCTCCGGGTCAACGCATATTTGATTCATGATACTGCTGA CTGGAAGGACCTGAACCTGAAGTTTGTATTGCAAATTTATCGGGACTATTACCTGACGGGTGATCAAGGC TTCCTGGAGGACATGTGGCCTGTGTGTCTGGCTGTGATGGAGTCCGAAATGAAGTTTGACAAGGACCAAG ATGGACTCATTGAGAATGGAGGCTACGCAGACCAGACCTATGATGCATGGGTCACCACAGGCCCCAGTGC TTACTGCGGAGGGCTGTGGCTGGCGGCAGTGGCTGTAATGGTTCAGATGGCTGTTCTGTGTGGGGCCCAA GATGTCCAGGAGAGGTTTGCTTCCATTCTCTGCCGAGGCCGAGAAGCTTATGAGAGACTGCTGTGGAACG GACGCTATTACAACTACGACAGCAGCTCCCATCCTCAGTCTCGGAGCATCATGTCTGACCAGTGTGCTGG GCAGTGGTTCCTGAGGGCCTGCGGCCTGGGAGAAGGAGACACTGAAGTATTTCCTACCCTGCATGTGGTC CGTGCTCTCCAAACCATCTTTGAACTCAACGTCCAAGCCTTTGCAGGAGGAGCCATGGGGGCCGTGAACG GGATGCACCCTCATGGTGTCCCTGATAGATCCAGTGTGCAGTCTGATGAAGTCTGGGTGGGTGTGGTCTA TGGGCTGGCAGCCACCATGATCCAAGAGGGCCTGACTTGGGAAGGTTTCCGGACAGCTGAAGGCTGTTAC CGCACTGTATGGGAACGCCTGGGCCTGGCTTTCCAGACCCCAGAGGCATACTGCCAGCAACAAGTGTTCC GCTCCCTGGCCTACATGCGGCCACTGAGCATCTGGGCCATGCAGCTGGCCCTGCAACAGCAGCAGCATAA AAAGAGCCGCAGGCCATCAGTCACACAAGGCACGGGACTAAGCACACAGCCTGAATGTGGACCAAAGAGA TCGCTGGCAAACCTCAATTCAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211175 representing NM_172692
Red=Cloning site Green=Tags(s) MVTCVPASEQVGCAERDSQVYCEDTGGTEAVRVTDCGSPEDSGPQDEPSYCNSEDSGQLMASYEGKARGY QVPPFGWRICLAHEFAEKRRPFQANNISLSNLVKHLGMGLRYLKWWYRKTHVEKKTPFIDMLNSLPLRQI YGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPNVLRSWNW GLCGYFAFYHALYPRAWTVYQLPGQNVTLTCRQVTPILPHDYQDSSLPVGVFVWDVENEGDETLDVSITF SMRNGLGGEDDAAGSLWNEPFRLEQGGTTVQGLLLHHPTPPNPYTMAVAARCTADTTVTHTTAFDPNGTG QQVWQDLLQDGQLDSPAGQSTPTQKGEGIAGAVCVSSKLLPRSRCCLEFSLAWDMPKIMFGAKSQVHYRR YTRFFGSDGDVAPALSHYALCHYADWEDRISAWQNPVLDDRTLPAWYKSALFNELYFLADGGTVWLEVPA DSLPEGLGGSMRQLRSTLQDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATLKE DLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQIYRDYYLTGDQG FLEDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDAWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQ DVQERFASILCRGREAYERLLWNGRYYNYDSSSHPQSRSIMSDQCAGQWFLRACGLGEGDTEVFPTLHVV RALQTIFELNVQAFAGGAMGAVNGMHPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEGCY RTVWERLGLAFQTPEAYCQQQVFRSLAYMRPLSIWAMQLALQQQQHKKSRRPSVTQGTGLSTQPECGPKR SLANLNSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172692 |
| ORF Size | 2754 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172692.3, NP_766280.2 |
| RefSeq Size | 3552 bp |
| RefSeq ORF | 2757 bp |
| Locus ID | 230101 |
| UniProt ID | Q69ZF3 |
| MW | 103.7 kDa |
| Gene Summary | Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide (PubMed:17080196, PubMed:23250757). Glucosylceramides are membrane glycosphingolipids that have a wide intracellular distribution (PubMed:23250757). They are the main precursors of more complex glycosphingolipids that play a role in cellular growth, differentiation, adhesion, signaling, cytoskeletal dynamics and membrane properties (PubMed:25803043). Also involved in the transglucosylation of cholesterol, transferring glucose from glucosylceramides, thereby modifying its water solubility and biological properties (PubMed:26724485). Under specific conditions, may catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485). Finally, may also play a role in the metabolism of bile acids (PubMed:17080196). It is able to hydrolyze bile acid 3-O-glucosides but also to produce bile acid-glucose conjugates thanks to a bile acid glucosyl transferase activity (PubMed:17080196). However, the relevance of both activities is unclear in vivo (PubMed:17080196).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205278 | Gba2 (untagged) - Mouse glucosidase beta 2 (Gba2), (10ug) |
CNY 2,000.00 |
|
| MG211175 | Gba2 (tGFP-tagged) - Mouse glucosidase beta 2 (Gba2) |
CNY 8,930.00 |
|
| MR211175L3 | Lenti ORF clone of Gba2 (Myc-DDK-tagged) - Mouse glucosidase beta 2 (Gba2) |
CNY 9,980.00 |
|
| MR211175L4 | Lenti ORF clone of Gba2 (mGFP-tagged) - Mouse glucosidase beta 2 (Gba2) |
CNY 9,980.00 |
