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Ulk1 (NM_009469) Mouse Tagged ORF Clone
CAT＃: MR211538
- TrueORF®
Ulk1 (Myc-DDK-tagged) - Mouse Unc-51 like kinase 1 (C. elegans) (Ulk1)
ORF Plasmid: tGFP
"NM_009469" in other vectors (3)
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CNY 7,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU041434; mKIAA0722; Unc51.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211538 representing NM_009469
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGGCCGCGGCGGCGTCGAGACCGTGGGCAAGTTCGAGTTCTCTCGCAAGGACCTGATTGGAC ACGGCGCCTTCGCGGTGGTCTTCAAGGGTCGACACCGCGAGAAGCACGACCTGGAGGTGGCCGTCAAATG CATTAACAAGAAGAACCTTGCCAAGTCCCAAACACTGCTGGGAAAGGAAATCAAAATCCTGAAGGAACTA AAGCACGAAAACATCGTGGCGCTGTATGACTTCCAGGAAATGGCTAATTCTGTCTACCTGGTCATGGAGT ATTGTAATGGTGGAGACCTGGCTGACTACCTGCACACTATGCGCACACTGAGTGAAGACACTGTCAGGCT TTTCCTACAGCAGATCGCTGGCGCCATGCGGCTGCTGCACAGCAAGGGCATCATCCACCGGGACCTGAAG CCCCAAAACATCCTGCTGTCCAACCCTGGGGGCCGCCGGGCCAACCCCAGCAACATCCGAGTCAAGATTG CTGACTTTGGATTCGCTCGGTACCTCCAGAGCAACATGATGGCGGCCACACTCTGTGGTTCTCCTATGTA CATGGCTCCTGAGGTCATTATGTCCCAGCACTACGATGGAAAGGCTGACCTGTGGAGCATTGGCACCATT GTCTACCAGTGTCTGACAGGGAAGGCCCCTTTTCAGGCCAGCAGCCCTCAGGATTTGCGCCTGTTTTATG AGAAGAACAAGACACTAGTTCCTGCCATCCCCCGGGAGACATCAGCTCCCCTGCGGCAGCTGCTCCTGGC TCTGTTGCAGCGGAACCACAAGGACCGCATGGACTTTGATGAATTTTTCCACCACCCTTTCTTGGATGCC AGCACCCCCATCAAGAAATCCCCACCTGTGCCTGTGCCCTCATATCCAAGCTCAGGGTCTGGCAGCAGCT CCAGCAGCAGCTCTGCCTCCCACCTGGCCTCTCCACCGTCCCTGGGGGAGATGCCACAGCTACAGAAGAC CCTTACCTCCCCAGCCGATGCTGCTGGCTTTCTTCAGGGCTCCCGGGACTCTGGTGGCAGCAGCAAAGAC TCCTGTGACACAGATGACTTTGTCATGGTCCCAGCCCAGTTTCCAGGTGATCTAGTTGCTGAGGCAGCCA GTGCCAAGCCCCCACCTGATAGCCTGCTGTGTAGTGGGAGCTCATTGGTGGCCTCTGCTGGCCTAGAGAG CCACGGCCGTACCCCCTCTCCCTCTCCGACCTGCAGCAGCTCTCCCAGCCCCTCTGGCCGGCCTGGCCCC TTCTCCAGCAACAGGTACGGTGCCTCGGTCCCCATTCCTGTCCCCACTCAGGTGCACAATTACCAGCGCA TCGAGCAAAACCTGCAATCGCCCACTCAACAGCAGACAGCCAGGTCCTCTGCCATCCGAAGGTCAGGGAG CACCAGCCCCCTGGGCTTTGGCCGGGCCAGCCCATCACCCCCCTCCCACACCGATGGAGCCATGCTGGCC AGGAAGCTGTCACTTGGAGGTGGCCGTCCCTACACACCTTCTCCCCAAGTGGGAACCATCCCAGAGCGAC CCAGCTGGAGCAGAGTGCCCTCCCCACAAGGAGCTGATGTGCGGGTTGGCAGGTCACCACGACCCGGTTC CTCTGTGCCTGAGCACTCTCCAAGAACCACTGGGCTGGGCTGCCGCCTGCACAGTGCCCCTAACCTGTCC GACTTCCATGTTGTGCGTCCCAAGCTGCCTAAGCCCCCAACAGACCCACTGGGAGCCACCTTTAGCCCAC CCCAGACCAGCGCACCCCAGCCATGCCCAGGGCTACAGTCTTGCCGGCCACTGCGTGGCTCACCTAAGCT GCCTGACTTCCTACAGCGGAGTCCCCTACCCCCCATCCTAGGCTCTCCTACCAAGGCCGGGCCCTCCTTT GACTTCCCCAAAACCCCCAGCTCTCAGAATTTGCTGACCCTGTTGGCTAGGCAGGGGGTAGTAATGACAC CACCTCGGAACCGTACACTGCCTGACCTCTCCGAGGCCAGTCCTTTCCATGGCCAGCAGCTGGGCTCTGG CCTTCGGCCCGCTGAAGACACCCGGGGTCCCTTTGGACGGTCCTTCAGCACCAGCCGCATTACGGACCTG CTGCTTAAGGCTGCATTTGGGACTCAGGCCTCTGACTCAGGCAGCACAGACAGCCTACAGGAGAAACCTA TGGAGATTGCTCCCTCTGCTGGCTTTGGAGGGACTCTGCATCCAGGAGCTCGTGGTGGAGGGGCCAGCAG CCCAGCACCTGTGGTATTTACTGTAGGCTCCCCACCCAGTGGTGCCACCCCACCCCAGAGTACCCGTACC AGAATGTTCTCAGTGGGCTCTTCCAGCTCCCTGGGCTCTACTGGCTCCTCCTCTGCCCGCCACTTAGTGC CTGGGGCCTGTGGAGAGGCCCCGGAGCTTTCTGCCCCAGGCCACTGCTGTAGCCTTGCTGACCCCCTTGC TGCCAACTTGGAGGGGGCTGTGACCTTCGAGGCTCCTGACCTCCCAGAGGAGACCCTCATGGAGCAAGAG CACACGGAAACCCTACACAGTCTGCGCTTCACACTAGCGTTTGCACAGCAAGTTCTGGAGATTGCAGCCC TGAAGGGAAGTGCCAGTGAGGCCGCCGGTGGCCCTGAGTACCAGCTCCAGGAAAGTGTGGTGGCTGACCA GATCAGTCAGTTGAGCCGAGAGTGGGGCTTTGCAGAGCAACTGGTTCTGTACTTGAAGGTGGCTGAGCTG CTGTCCTCAGGCCTACAGACTGCCATTGACCAGATTCGAGCTGGCAAACTCTGCCTTTCATCTACTGTGA AGCAGGTGGTACGCAGACTAAATGAGCTGTACAAGGCCAGCGTGGTATCCTGCCAGGGCCTCAGCTTGCG ACTTCAGCGCTTCTTTCTGGACAAACAACGGCTGCTGGACGGGATCCATGGTGTCACTGCAGAGCGGCTC ATCCTCAGCCATGCTGTGCAAATGGTACAATCAGCTGCCCTTGATGAGATGTTCCAGCACCGAGAGGGCT GTGTACCGAGATATCACAAAGCCCTGCTATTGCTGGAGGGGTTGCAGCACACTCTCACGGACCAGGCAGA CATTGAGAACATTGCCAAATGCAAGCTGTGCATTGAGAGGAGACTCTCGGCCCTGCTGAGTGGTGTCTAT GCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211538 representing NM_009469
Red=Cloning site Green=Tags(s) MEPGRGGVETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKEL KHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHTMRTLSEDTVRLFLQQIAGAMRLLHSKGIIHRDLK PQNILLSNPGGRRANPSNIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTI VYQCLTGKAPFQASSPQDLRLFYEKNKTLVPAIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDA STPIKKSPPVPVPSYPSSGSGSSSSSSSASHLASPPSLGEMPQLQKTLTSPADAAGFLQGSRDSGGSSKD SCDTDDFVMVPAQFPGDLVAEAASAKPPPDSLLCSGSSLVASAGLESHGRTPSPSPTCSSSPSPSGRPGP FSSNRYGASVPIPVPTQVHNYQRIEQNLQSPTQQQTARSSAIRRSGSTSPLGFGRASPSPPSHTDGAMLA RKLSLGGGRPYTPSPQVGTIPERPSWSRVPSPQGADVRVGRSPRPGSSVPEHSPRTTGLGCRLHSAPNLS DFHVVRPKLPKPPTDPLGATFSPPQTSAPQPCPGLQSCRPLRGSPKLPDFLQRSPLPPILGSPTKAGPSF DFPKTPSSQNLLTLLARQGVVMTPPRNRTLPDLSEASPFHGQQLGSGLRPAEDTRGPFGRSFSTSRITDL LLKAAFGTQASDSGSTDSLQEKPMEIAPSAGFGGTLHPGARGGGASSPAPVVFTVGSPPSGATPPQSTRT RMFSVGSSSSLGSTGSSSARHLVPGACGEAPELSAPGHCCSLADPLAANLEGAVTFEAPDLPEETLMEQE HTETLHSLRFTLAFAQQVLEIAALKGSASEAAGGPEYQLQESVVADQISQLSREWGFAEQLVLYLKVAEL LSSGLQTAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDGIHGVTAERL ILSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHTLTDQADIENIAKCKLCIERRLSALLSGVY A TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009469 |
| ORF Size | 3153 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009469.3, NP_033495.2 |
| RefSeq Size | 5215 bp |
| RefSeq ORF | 3156 bp |
| Locus ID | 22241 |
| UniProt ID | O70405 |
| MW | 112.9 kDa |
| Gene Summary | Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211538 | Ulk1 (tGFP-tagged) - Mouse Unc-51 like kinase 1 (C. elegans) (Ulk1) |
CNY 9,220.00 |
|
| MR211538L3 | Lenti ORF clone of Ulk1 (Myc-DDK-tagged) - Mouse Unc-51 like kinase 1 (C. elegans) (Ulk1) |
CNY 10,260.00 |
|
| MR211538L4 | Lenti ORF clone of Ulk1 (mGFP-tagged) - Mouse Unc-51 like kinase 1 (C. elegans) (Ulk1) |
CNY 10,260.00 |
